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Comparative Proteomics of Symbiotic and Aposymbiotic Juvenile Soft Corals

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Abstract

The symbiotic association between corals and photosynthetic unicellular algae is of great importance in coral reef ecosystems. The study of symbiotic relationships is multidisciplinary and involves research in phylogeny, physiology, biochemistry, and ecology. An intriguing phase in each symbiotic relationship is its initiation, in which the partners interact for the first time. The examination of this phase in coral–algae symbiosis from a molecular point of view is still at an early stage. In the present study we used 2-dimensional polyacrylamide gel electrophoresis to compare patterns of proteins synthesized in symbiotic and aposymbiotic primary polyps of the Red Sea soft coral Heteroxenia fuscescens. This is the first work to search for symbiosis-specific proteins during the natural onset of symbiosis in early host ontogeny. The protein profiles reveal changes in the host soft coral proteome through development, but surprisingly virtually no changes in the host proteome as a function of symbiotic state.

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Acknowledgments

We thank E.A. Verde and C.L. Mitchelmore for valuable assistance in the laboratory. We thank T. Yacobovitch, A. Maoz, and I. Adler for their help with collection of animals. We are grateful to V. Wexler for graphic assistance and N. Paz for editorial assistance. We thank the staff of the Interuniversity Institute of Eilat for their kind hospitality and facilities. This research was in part supported by grant 1998458 from the United States–Israel Binational Science Foundation (BSF), Jerusalem, Israel, and by a grant from the Basic Research Fund of Tel Aviv University. O.B. was supported by a fellowship granted by Tobias Landau Foundation and Rieger JNF Fellow in Environmental Studies.

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Authors and Affiliations

  1. Department of Zoology, George S. Wise Faculty of Life Sciences, Tel Aviv University, Ramat Aviv, Tel Aviv, 69978, Israel

    O. Barneah & Y. Benayahu

  2. Department of Zoology, Oregon State University, Corvallis, OR, 97331, USA

    V. M. Weis

Authors
  1. O. Barneah
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  2. Y. Benayahu
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  3. V. M. Weis
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Correspondence to O. Barneah.

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Barneah, O., Benayahu, Y. & Weis, V.M. Comparative Proteomics of Symbiotic and Aposymbiotic Juvenile Soft Corals. Mar Biotechnol 8, 11–16 (2006). https://doi.org/10.1007/s10126-004-5120-8

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  • DOI: https://doi.org/10.1007/s10126-004-5120-8

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