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Cloning of Two Crustacean Hyperglycemic Hormone Isoforms in Freshwater Giant Prawn (Macrobrachium rosenbergii): Evidence of Alternative Splicing

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Abstract

A full-length chh cDNA was cloned from the eyestalk of Macrobrachium rosenbergii. The 991-bp cDNA contains an open reading frame of 408 bp that encodes the prepro-CHH. The tissue-specific expression pattern was examined by reverse transcriptase-polymerase chain reaction. Positive signals were detected in the eyestalk, heart, gills, antennal glands, and thoracic ganglion but not in muscle and hepatopancreas. However, two types of products were observed. The nucleotide sequences revealed the existence of 2 chh transcripts, named chh and chh-l, respectively. Direct sequence evidence suggests that these two isoforms come from a Chh gene transcribed in an alternative splicing manner. The Mar-Chh gene consists of 4 exons. The eyestalk transcript (chh) contains exons I, II, and IV, whereas the chh-l transcript in heart, gills, antennal glands, and thoracic ganglion contains all 4 exons. The appearance of exon III in chh-l cDNA changes the sequence content in the latter half of the mature peptide, starting within the codon of the 40th residue, arginine. The amino acid sequence deduced from exon III matched no homologue in public protein databases, while the 2 cysteine residues in this segment preserved the positional conservation characters of CHH neuropeptide family members. The common organization of Chh genes between palaemonid, brachyuran, and astacus crustaceans suggests that the Chh gene has a 4-exon structure in these species.

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References

  1. A.A. Abramowitz F.L. Hisaw D.N. Papandrea (1944) ArticleTitleThe occurrence of a diabetogenic factors in the eyestalks of crustaceans. Biol Bull 86 1–5 Occurrence Handle1:CAS:528:DyaH2cXhvFSlsg%3D%3D

    CAS  Google Scholar 

  2. M.B. Aguilar D. Soyez R. Falchetto D. Amott J. Shabanowitz D.F. Hunt A. Huberman (1995) ArticleTitleAmino acid sequence of the minor isomorph of the crustacean hyperglycemic hormone (CHH-II) of the Mexican crayfish Procambarus bouvieri (Ortmann): presence of a D-amino acid. Peptides 16 1375–1383 Occurrence Handle10.1016/0196-9781(95)02024-1 Occurrence Handle1:CAS:528:DyaK2MXhtVShsL7N Occurrence Handle8745046

    Article  CAS  PubMed  Google Scholar 

  3. S.F. Altschul T.L. Madden A.A. Schaffer J.H. Zhang Z. Zhang W. Miller D.J. Lipman (1997) ArticleTitleGapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 25 3389–3402 Occurrence Handle9254694

    PubMed  Google Scholar 

  4. N. Audsley C. McIntosh J.E. Phillips (1992) ArticleTitleIsolation of a neuropeptide from locust corpus cardiacum which influences ileal transport. J Exp Biol 173 261–274 Occurrence Handle1:CAS:528:DyaK3sXhs1Gntbk%3D Occurrence Handle1487715

    CAS  PubMed  Google Scholar 

  5. R.D. Barnes F.W. Harrison (1992) Introduction to the Decapoda. F.W. Harrison (Eds) Microscopic Anatomy of Invertebrates. Wiley-Liss Inc New York, N.Y. 10:1–6

    Google Scholar 

  6. E.S. Chang G.D. Prestwich M.J. Bruce (1990) ArticleTitleAmino acid sequence of a peptide with both molt-inhibiting and hyperglycemic activities in the lobster, Homarus americanus. Biochem Biophys Res Commun 171 818–826 Occurrence Handle1:CAS:528:DyaK3MXlslKluw%3D%3D Occurrence Handle2169734

    CAS  PubMed  Google Scholar 

  7. E.S. Chang R. Keller S.A. Chang (1998) ArticleTitleQuantification of crustacean hyperglycemic hormone by ELISA in hemolymph of the lobster, Homarus americanus, following various stresses. Gen Comp Endocrinol 111 359–366 Occurrence Handle10.1006/gcen.1998.7120 Occurrence Handle1:CAS:528:DyaK1cXls1arsL4%3D Occurrence Handle9707481

    Article  CAS  PubMed  Google Scholar 

  8. E.S. Chang S.A. Chang B.S. Beltz E.A. Kravitz (1999) ArticleTitleCrustacean hyperglycemic hormone in the lobster nervous system: localization and release from cells in the subesophageal ganglion and thoracic second roots. J Comp Neurol 414 50–56 Occurrence Handle10.1002/(SICI)1096-9861(19991108)414:1<50::AID-CNE4>3.0.CO;2-Q Occurrence Handle1:CAS:528:DyaK1MXmslGhsr8%3D Occurrence Handle10494077

    Article  CAS  PubMed  Google Scholar 

  9. E.L. Charles S.F. Altschul M.S. Boguski J.S. Liu A.F. Neuwald J.C. Wootton (1993) ArticleTitleDetecting subtle sequence signals: a Gibbs sampling strategy for multiple alignment. Science 262 208–214 Occurrence Handle1:CAS:528:DyaK3sXms1Gjsrk%3D Occurrence Handle8211139

    CAS  PubMed  Google Scholar 

  10. J.S. Chung H. Dircksen S.G. Webster (1999) ArticleTitleA remarkable, precisely timed release of hyperglycemic hormone from endocrine cells in the gut is associated with ecdysis in the crab Carcinus maenas. Proc Natl Acad Sci USA 96 13103–13107 Occurrence Handle10.1073/pnas.96.23.13103 Occurrence Handle1:CAS:528:DyaK1MXns1Gms7c%3D Occurrence Handle10557280

    Article  CAS  PubMed  Google Scholar 

  11. D.P. de Kleijn K.P. Janssen S.L. Waddy R. Hegeman W.Y. Lai G.J. Martens F. van Herp (1998) ArticleTitleExpression of the crustacean hyperglycaemic hormones and the gonad-inhibiting hormone during the reproductive cycle of the female American lobster Homarus americanus. J Endocrinol 156 291–298 Occurrence Handle1:CAS:528:DyaK1cXhtV2ktrY%3D Occurrence Handle9518875

    CAS  PubMed  Google Scholar 

  12. H. Dircksen D. Bocking U. Heyn C. Mandel J.S. Chung G. Baggerman P. Verhaert S. Daufeldt T. Plosch P.P. Jaros E. Waelkens R. Keller S.G. Webster (2001) ArticleTitleCrustacean hyperglycaemic hormone (CHH)-like peptides and CHH-precursor-related peptides from pericardial organ neurosecretory cells in the shore crab, Carcinus maenas, are putatively spliced and modified products of multiple genes. Biochem J 356 159–170 Occurrence Handle10.1042/0264-6021:3560159 Occurrence Handle1:CAS:528:DC%2BD3MXjvF2itL8%3D Occurrence Handle11336648

    Article  CAS  PubMed  Google Scholar 

  13. M. Fingerman (1992) Glands and secretion. F.W. Harrison (Eds) Microscopic Anatomy of Invertebrates, Wiley-Liss Inc New York, N.Y. 10:345–394

    Google Scholar 

  14. P.L. Gu S.M. Chan (1998) ArticleTitleThe shrimp hyperglycemic hormone-like neuropeptide is encoded by multiple copies of genes arranged in a cluster. FEBS Lett 441 397–403 Occurrence Handle10.1016/S0014-5793(98)01573-7 Occurrence Handle1:CAS:528:DyaK1MXhslWktw%3D%3D Occurrence Handle9891979

    Article  CAS  PubMed  Google Scholar 

  15. P.L. Gu K.L. Yu S.M. Chan (2000) ArticleTitleMolecular characterization of an additional shrimp hyperglycemic hormone: cDNA cloning, gene organization, expression and biological assay of recombinant proteins. FEBS Lett 472 122–128 Occurrence Handle1:CAS:528:DC%2BD3cXislarsbw%3D Occurrence Handle10781818

    CAS  PubMed  Google Scholar 

  16. J.L. Kallen S.L. Abrahamse F. van Herp (1990) ArticleTitleCircadian rhythmicity of the crucstacan hyperglycemic hormone CHH in the hemolymph of the crayfish. Biol Bull 179 351–357 Occurrence Handle1:CAS:528:DyaK3MXkt1KhtLk%3D

    CAS  Google Scholar 

  17. F.I. Kamemoto (1991) ArticleTitleNeuroendocrinology of osmoregulation in crabs. Zool Sci (Tokyo) 8 827–833 Occurrence Handle1:CAS:528:DyaK38XotVKmtA%3D%3D

    CAS  Google Scholar 

  18. F.I. Kamemoto R.E. Tullis (1972) ArticleTitleHydromineral regulation in decapod crustacean. Gen Comp Endocrinol 3 299–307

    Google Scholar 

  19. R. Keller D. Sedlmeier (1988) A metabolic hormone in crustaceans: the hyperglycemic neuropeptide. H. Laufer R.G.H. Downer (Eds) Endocrinology of Selected Invertebrate Types. A R Liss New York, N.Y. 2:315–326

    Google Scholar 

  20. C.M. Kuo Y.H. Yang (1999) ArticleTitleHyperglycemic responses to cold shock in the freshwater giant prawn, Macrobrachium rosenbergii. J Comp PhysiolB 169 49–54 Occurrence Handle10.1007/s003600050192

    Article  Google Scholar 

  21. C. Lacombe P. Greve G. Martin (1999) ArticleTitleOverview on the sub-grouping of the crustacean hyperglycemic hormone family. Neuropeptides 33 71–80 Occurrence Handle10.1054/npep.1999.0016 Occurrence Handle1:CAS:528:DyaK1MXjsVyitLw%3D Occurrence Handle10657474

    Article  CAS  PubMed  Google Scholar 

  22. C.Y. Lin S.H. Chen G.H. Kou C.M. Kuo (1998) ArticleTitleIdentification and characterization of a hyperglycemic hormone from freshwater giant prawn, Macrobrachium rosenbergii. Comp Biochem Physiol A 121 315–321

    Google Scholar 

  23. L. Liu H. Laufer (1996) ArticleTitleIsolation and characterization of sinus gland neuropeptides with both mandibular organ inhibiting and hyperglycemic effects from the spider crab Libinia emarginata. Arch Int Physiol Biochim 32 375–385 Occurrence Handle10.1002/(SICI)1520-6327(1996)32:3/4<375::AID-ARCH10>3.3.CO;2-0 Occurrence Handle1:CAS:528:DyaK28Xks1GgtL4%3D

    Article  CAS  Google Scholar 

  24. S. Lorenzon P.G. Giulianini E.A. Ferrero (1997) ArticleTitleLipopolysaccharide-induced hyperglycemia is mediated by CHH release in crustaceans. Gen Comp Endocrinol 108 395–405 Occurrence Handle10.1006/gcen.1997.6986 Occurrence Handle1:CAS:528:DyaK1cXhvFSr Occurrence Handle9405116

    Article  CAS  PubMed  Google Scholar 

  25. S. Lorenzon M. Francese E.A. Ferrero (2000) ArticleTitleHeavy metal toxicity and differential effects on the hyperglycemic stress response in the shrimp Palaemon elegans. Arch Environ Contam Toxicol 39 167–176 Occurrence Handle10.1007/s002440010093 Occurrence Handle1:CAS:528:DC%2BD3cXmtV2gs70%3D Occurrence Handle10871419

    Article  CAS  PubMed  Google Scholar 

  26. A. Macins J. Meredith Y. Zhao H.W. Brock J.E. Phillips (1999) ArticleTitleOccurrence of ion transport peptide (ITP) and ion transport-like peptide (ITP-L) in Orthopteroids. Arch Insect Biochem Physiol 40 107–118 Occurrence Handle10.1002/(SICI)1520-6327(1999)40:2<107::AID-ARCH5>3.3.CO;2-2 Occurrence Handle1:CAS:528:DyaK1MXhs12ksb4%3D Occurrence Handle10077829

    Article  CAS  PubMed  Google Scholar 

  27. S. Morris (2001) ArticleTitleNeuroendocrine regulation of osmoregulation and the evolution of air-breathing in decapod crustaceans. J Exp Biol 204 979–989 Occurrence Handle1:CAS:528:DC%2BD3MXislClt7k%3D Occurrence Handle11171421

    CAS  PubMed  Google Scholar 

  28. A. Muramoto (1988) Endocrine control of water balance in decapod crustaceans. H. Laufer R.G.H. Downer (Eds) Endocrinology of Selected Invertebrate Types. A R Liss New York, N.Y. 2:341–356

    Google Scholar 

  29. H. Nielsen B. Soren G. von Heijen (1999) ArticleTitleMachine learning approaches for the prediction of signal peptides and other protein sorting signals. Protein Eng 12 3–9 Occurrence Handle10.1093/protein/12.1.3 Occurrence Handle1:CAS:528:DyaK1MXht1KhtbY%3D Occurrence Handle10065704

    Article  CAS  PubMed  Google Scholar 

  30. C. Ollivaux D. Soyez (2000) ArticleTitleDynamics of biosynthesis and release of crustacean hyperglycemic hormone isoforms in the X-organ–sinus gland complex of the crayfish Orconectes limosus. Eur J Biochem 267 5106–5114 Occurrence Handle10.1046/j.1432-1327.2000.01574.x Occurrence Handle1:CAS:528:DC%2BD3MXitlegurY%3D Occurrence Handle10931194

    Article  CAS  PubMed  Google Scholar 

  31. C. Pierrot D. Soyez F. van Herp M. Gompel G. Skaret E. Grousset G. Charmantier (2000) ArticleTitleInvolvement of crustacean hyperglycemic hormone in the control of gill ion transport in the crab Pachygrapsus marmoratus. Gen Comp Endocrinol 119 3401–350

    Google Scholar 

  32. P.S. Reddy M. Devi R. Sarojini R. Nagabhushanam M. Fingerman (1994) ArticleTitleCadmium chloride induced hyperglycemia in the red swamp crayfish, Procambarus clarkii: Possible role of crustacean hyperglycemia hormone. Comp Biochem Physiol C 107 57–61 Occurrence Handle10.1016/0300-9629(94)90273-9

    Article  Google Scholar 

  33. P.S. Reddy R.V. Katyayani M. Fingerman (1996) ArticleTitleCadmium and naphthalene-induced hyperglycemia in the fiddler crab, Uca pugilator: differential modes of action on the neuroendocrine system. Bull Environ Contam Toxicol 56 425–431 Occurrence Handle10.1007/s001289900061 Occurrence Handle1:CAS:528:DyaK28XlsFWjsA%3D%3D Occurrence Handle8825965

    Article  CAS  PubMed  Google Scholar 

  34. P. Rice I. Longden A. Bleasby (2000) ArticleTitleEMBOSS: The European Molecular Biology Open Software Suite. Trends Genet 16 276–277 Occurrence Handle10827456

    PubMed  Google Scholar 

  35. B. Rost C. Sander (1994) ArticleTitleCombining evolutionary information and neural networks to predict protein secondary structure. Proteins 19 55–77 Occurrence Handle1:CAS:528:DyaK2MXhtlWqtw%3D%3D Occurrence Handle8066087

    CAS  PubMed  Google Scholar 

  36. A.A. Salamov T. Nishikawa M.B. Swindells (1998) ArticleTitleAssessing protein coding region integrity in cDNA sequencing projects. Bioinformatics 14 384–390

    Google Scholar 

  37. J. Sambrook D.W. Russell (2001) Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Cold Spring Harbor, N.Y.

    Google Scholar 

  38. E.A. Santos R. Keller (1993a) ArticleTitleEffect of exposure to atmospheric air on blood glucose and lactate concentrations in two crustacean species: a role of the crustacean hyperglycemic hormone (CHH). Comp Biochem Physiol A 106 343–347 Occurrence Handle1:CAS:528:DC%2BD3MXktFKhsrY%3D Occurrence Handle11361190

    CAS  PubMed  Google Scholar 

  39. E.A. Santos R. Keller (1993b) ArticleTitleRegulation of circulating levels of the crustacean hyperglycemic hormone: evidence for a dual feedback control system. J Comp Physiol B 163 374–379 Occurrence Handle1:CAS:528:DyaK2cXitFeju7s%3D

    CAS  Google Scholar 

  40. E.A. Santos L.E. Nery R. Keller A.A. Goncalves (1997) ArticleTitleEvidence for the involvement of the crustacean hyperglycemic hormone in the regulation of lipid metabolism. Physiol Zool 70 415–420 Occurrence Handle1:CAS:528:DyaK2sXktV2jtrk%3D Occurrence Handle9237301

    CAS  PubMed  Google Scholar 

  41. D. Sedlmeier (1982) ArticleTitleThe mode of action of the crustacean neurosecretory hyperglycemic hormone (CHH), II: involvement of glycogen synthase. Gen Comp Endocrinol 47 426–432 Occurrence Handle1:CAS:528:DyaL38Xks12ksb0%3D Occurrence Handle6288511

    CAS  PubMed  Google Scholar 

  42. M. Sefiani J.P. Le Caer D. Soyez (1996) ArticleTitleCharacterization of hyperglycemic and molt-inhibiting activity from sinus glands of the penaeid shrimp Penaeus vannamei. Gen Comp Endocrinol 103 41–53 Occurrence Handle10.1006/gcen.1996.0092 Occurrence Handle1:CAS:528:DyaK28Xktlegurg%3D Occurrence Handle8812330

    Article  CAS  PubMed  Google Scholar 

  43. W. Sithigorngul S. Jaideechoey W. Saraithongkum S. Longyant P. Sithigorngul (1999) ArticleTitlePurification and characterization of an isoform of crustacean hyperglycemic hormone from the eyestalk of Macrobrachium rosenbergii. J Exp Zool 284 217–224 Occurrence Handle1:CAS:528:DyaK1MXksVyjsbw%3D Occurrence Handle10404650

    CAS  PubMed  Google Scholar 

  44. H. Sonobe T. Nishimura M. Sonobe T. Nakatsuji R. Yanagihara T. Kawakami S. Aimoto (2001) ArticleTitleThe molt-inhibiting hormone in the American crayfish Procambarus clarkii: its chemical synthesis and biological activity. Gen Comp Endocrinol 121 196–204 Occurrence Handle10.1006/gcen.2000.7586 Occurrence Handle1:CAS:528:DC%2BD3MXhtFenu7k%3D Occurrence Handle11178885

    Article  CAS  PubMed  Google Scholar 

  45. D. Soyez F. van Herp J. Rossier J.P. Le Caer C.P. Tensen R. Lafont (1994) ArticleTitleEvidence for a conformational polymorphism of invertebrate neurohormones. D-amino acid residue in crustacean hyperglycemic peptides. J Biol Chem 269 18295–18298 Occurrence Handle1:CAS:528:DyaK2cXlslehu7o%3D Occurrence Handle8034574

    CAS  PubMed  Google Scholar 

  46. D. Soyez J.Y. Toullec C. Ollivaux G. Geraud (2000) ArticleTitleL to D amino acid isomerization in a peptide hormone is a late post-translational event occurring in specialized neurosecretory cells. J Biol Chem 275 37870–37875 Occurrence Handle10.1074/jbc.M007302200 Occurrence Handle1:CAS:528:DC%2BD3cXoslWgtbY%3D Occurrence Handle10993902

    Article  CAS  PubMed  Google Scholar 

  47. G.D. Stentiford E.S. Chang S.A. Chang D.M. Neil (2001) ArticleTitleCarbohydrate dynamics and the crustacean hyperglycemic hormone (CHH): effects of parasitic infection in Norway lobsters (Nephrops norvegicus). Gen Comp Endocrinol 121 13–22 Occurrence Handle10.1006/gcen.2000.7575 Occurrence Handle1:CAS:528:DC%2BD3MXmslarsA%3D%3D Occurrence Handle11161766

    Article  CAS  PubMed  Google Scholar 

  48. C.P. Tensen K.P.C. Janssen F. van Herp (1989) ArticleTitleIsolation, characterization and physiological specificity of the crustacean hyperglycemic factors from the sinus gland of the lobster Homarus americanus (Milne-Edwards). Invert Reprod Dev 16 155–164 Occurrence Handle1:CAS:528:DyaK3MXit1Crsr4%3D

    CAS  Google Scholar 

  49. J.Y. Toullec N. Kamech D. Gallois M. Maibeche V. Papon M. Boscameric D. Soyez (2002) ArticleTitleMolecular cloning and cellular expression of crustacean PC2-like prohormone convertase. Biochim Biophys Acta 1574 145–l51 Occurrence Handle10.1016/S0167-4781(01)00356-6 Occurrence Handle1:CAS:528:DC%2BD38XktVKgtb4%3D Occurrence Handle11955623

    Article  CAS  PubMed  Google Scholar 

  50. S.G. Webster (1996) ArticleTitleMeasurement of crustacean hyperglycaemic hormone levels in the edible crab Cancer pagurus during emersion stress. J Exp Biol 199 1579–1585 Occurrence Handle1:CAS:528:DyaK28XltFWru78%3D Occurrence Handle9319482

    CAS  PubMed  Google Scholar 

  51. S.G. Webster R. Keller (1986) ArticleTitlePurification, characterization and amino acid composition of the putative moult-inhibiting hormone (MIH) of Carcinus maenas (Crustacea, Decapoda). J Comp Physiol 156 617–624 Occurrence Handle1:CAS:528:DyaL28Xls12hsbo%3D

    CAS  Google Scholar 

  52. S.J. Wheelan D.M. Chruch J.M. Ostell (2001) ArticleTitleSpidey: a tool for mRNA-to-genomic alignments. Genome Res 11 1952–1957

    Google Scholar 

  53. W.J. Yang K. Aida A. Terauchi H. Sonobe H. Nagasawa (1996) ArticleTitleAmino acid sequence of a peptide with molt-inhibiting activity from the Kuruma prawn Penaeus japonicus. Peptides 17 197–202 Occurrence Handle10.1016/0196-9781(95)02122-1 Occurrence Handle1:CAS:528:DyaK28XhvFOltr8%3D Occurrence Handle8801521

    Article  CAS  PubMed  Google Scholar 

  54. W.J. Yang K. Aida H. Nagasawa (1997) ArticleTitleAmino acid sequences and multiple hyperglycemic hormones from the Kuruma prawn, Penaeus japonicus. Peptides 18 479–485 Occurrence Handle10.1016/S0196-9781(96)00332-4 Occurrence Handle1:CAS:528:DyaK2sXktVKisrk%3D Occurrence Handle9210164

    Article  CAS  PubMed  Google Scholar 

  55. A. Yasuda Y. Yasuda T. Fujita Y. Naya (1994) ArticleTitleCharacterization of crustacean hyperglycemic hormone from the crayfish (Procambarus clarkii): multiplicity of molecular forms by stereoinversion and diverse functions. Gen Comp Endocrinol 95 387–398 Occurrence Handle10.1006/gcen.1994.1138 Occurrence Handle1:CAS:528:DyaK2cXmvFGgurg%3D Occurrence Handle7821776

    Article  CAS  PubMed  Google Scholar 

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Acknowledgements

We are grateful to Dr. Huang Fore-Lien for his critical reading and suggestions on the manuscript. Our thanks also go to Mrs. Fang Mei-Jeng for her assistance on autosequencing. This work was supported by a research grant from National Science Council ROC, to Dr. Kuo.

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Authors and Affiliations

  1. Institute of Zoology, National Taiwan University, Taipei, Taiwan

    Shu-Hwa Chen

  2. Division of Biostatistics and Bioinformatics, National Health Research Institute, Taipei, Taiwan

    Chung-Yen Lin

  3. Marine Research Station, Institute of Zoology, Academia Sinica, No. 23–10, Dah-Uen Rd., Jiaw-Shi, Ilan, Taiwan

    Ching Ming Kuo

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Chen, SH., Lin, CY. & Kuo, C. Cloning of Two Crustacean Hyperglycemic Hormone Isoforms in Freshwater Giant Prawn (Macrobrachium rosenbergii): Evidence of Alternative Splicing . Mar. Biotechnol. 6, 83–94 (2004). https://doi.org/10.1007/s10126-003-0014-8

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