Marine Biotechnology

, Volume 6, Issue 1, pp 17–25 | Cite as

Antiviral Effects of Sulfated Exopolysaccharide from the Marine Microalga Gyrodinium impudicum Strain KG03

  • Joung Han Yim
  • Sung Jin Kim
  • Se Hun Ahn
  • Chong Kyo Lee
  • Ki Tae Rhie
  • Hong Kum LeeEmail author


The sulfated exopolysaccharide p-KG03, which is produced by the marine microalga Gyrodinium impudicum strain KG03, exhibited impressive antiviral activity in vitro (EC50 = 26.9 µg/ml) against the encephalomyocarditis virus (EMCV). Depending on the p-KG03 concentration, the development of cytopathic effects in EMCV-infected HeLa cells was either inhibited completely or slowed. Moreover, p-KG03 did not show any cytotoxic effects on HeLa cells, even at concentrations up to 1000 µg/ml. The polysaccharide was purified by repeated precipitation in ethanol, followed by gel filtration. The p-KG03 polysaccharide had a molecular weight of 1.87 × 107, and was characterized as a homopolysaccharide of galactose with uronic acid (2.96% wt/wt) and sulfate groups (10.32% wt/wt). The biological activities of p-KG03 suggest that sulfated metabolites from marine organisms are a rich source of antiviral agents. This is the first reported marine source of antiviral sulfated polysaccharides against EMCV. The p-KG03 polysaccharide may be useful in the development of marine bioactive exopolysaccharide for biotechnological and pharmaceutical products.


marine microalga Gyrodinium impudicum sulfated exopolysaccharide antiviral activity encephalomyocarditis virus 



This study was supported by a grant to H.K.L. from the Development of Marine Novel Compounds Program of the Korean Ministry of Maritime Affairs and Fisheries.


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Copyright information

© Springer-Verlag 2003

Authors and Affiliations

  • Joung Han Yim
    • 1
  • Sung Jin Kim
    • 1
  • Se Hun Ahn
    • 1
  • Chong Kyo Lee
    • 2
  • Ki Tae Rhie
    • 3
  • Hong Kum Lee
    • 1
    Email author
  1. 1.Microbiology LaboratoryKorea Ocean Research & Development Institute, Ansan P.O. Box 29, Seoul 425-600Republic of Korea
  2. 2.Pharmaceutical Screening CenterKorea Research Institute of Chemical Technology, 100 Jang Dong, Taejon 305-606Republic of Korea
  3. 3.Department of BiologyKyung-Hee University, 1 Hoegi-Dong, Dongdaemoon-Gu, Seoul 130-701Korea

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