Abstract
The QM gene was originally identified as a putative tumor suppressor gene from a Wilms’ tumor cell line by subtractive hybridization assay. Later studies showed that the QM protein is multifunctional, involved in cell growth and differentiation, energy metabolism, respiration, and cytoskeletal function. In this report a full-length complementary DNA encoding a QM counterpart in pearl oyster (Pinctada fucata) was isolated. Phylogenetic analysis shows that oyster QM is more closely related to its insect homologues than to the mammalian homologues. Analysis of the tissue expression pattern of the oyster QM gene showed that oyster QM messenger RNA is expressed in all tissues tested, with highest levels in the digestive gland and mantle. Furthermore, we expressed the QM protein in Escherichia coli; Western blotting showed that the antibody of human QM is immunoreactive to the expressed oyster QM protein. Incubation of the oyster QM with Zn2+ resulted in the reduction of intrinsic emission fluorescence and a red-shift in the λmax emission, indicating the occurrence of Zn2+-induced conformational changes. This evidence presents a possible mechanism for the critical function of zinc ion in the interaction of QM with Jun.
Similar content being viewed by others
References
P. Angel E.A. Allegretto S.T. Okino K. Hattori W.J. Boyle T. Hunter M. Karin (1988) ArticleTitleOncogene jun encodes a sequence-specific trans-activator similar to AP-1. Nature 332 166–171 Occurrence Handle1:CAS:528:DyaL1cXlt1ens7s%3D Occurrence Handle3347253
F.M. Ausubel R. Brent R.E. Kingston D.D. Moore J.G. Seidman J.A. Smith K. Struhl (1999) Short Protocols in Molecular Biology, 4th ed. John Wiley & Sons Inc. New York, N.Y.
Y.L. Chan J.J. Diaz L. Denoroy J.J. Madjar I.G. Wool (1996) ArticleTitleThe primary structure of rat ribosomal protein L10: relationship to a Jun-binding protein and to a putative Wilms’ tumor suppressor. Biochem Biophys Res Commun 225 952–956 Occurrence Handle10.1006/bbrc.1996.1277 Occurrence Handle1:CAS:528:DyaK28Xlt1ajsbg%3D Occurrence Handle8780716
F.A. Dick B.L. Trumpower (1998) ArticleTitleHeterologous complementation reveals that mutant alleles of QSR1 render 60S ribosomal subunits unstable and translationally inactive. Nucleic Acids Res 26 2442–2448 Occurrence Handle10.1093/nar/26.10.2442 Occurrence Handle1:CAS:528:DyaK1cXjvFWmtLs%3D Occurrence Handle9580698
F.A. Dick S. Karamanou B.L. Trumpower (1997) ArticleTitle QSR1, an essential yeast gene with a genetic relationship to a subunit of the mitochondrial cytochrome bc 1 complex, codes for a 60S ribosomal subunit protein. J Biol Chem 272 13372–13379 Occurrence Handle10.1074/jbc.272.20.13372 Occurrence Handle1:CAS:528:DyaK2sXjtlart7Y%3D Occurrence Handle9148960
S.F. Dowdy K.M. Lai B.E. Weissman Y. Matsui B.L.M. Hogan E.J. Stanbridge (1991) ArticleTitleThe isolation and characterization of a novel cDNA demonstrating an altered mRNA level in nontumorigenic Wilms’ microcell hybrid cells. Nucleic Acids Res 19 5763–5769 Occurrence Handle1:CAS:528:DyaK3sXhvVWitg%3D%3D Occurrence Handle1658743
D.P. Eisinger F.A. Dick B.L. Trumpower (1997) ArticleTitleQsr1p, a 60S ribosomal subunit protein, is required for joining of 40S and 60S subunits. Mol Cell Biol 17 5136–5145 Occurrence Handle1:CAS:528:DyaK2sXlslartr0%3D Occurrence Handle9271391
H. Green A.E. Canfield M.C. Hillarby M.E. Grant R.P. Boot-handford A.J. Freemont G.A. Wallis (2000) ArticleTitleThe ribosomal protein QM is expressed differentially during vertebrate endochondral bone development. J Bone Miner Res 15 1066–1075 Occurrence Handle1:CAS:528:DC%2BD3cXktFKhur8%3D Occurrence Handle10841175
J.S. Hwang T.W. Goo E.Y. Yun J.H. Lee S.W. Kang K.Y. Kim O.Y. Kwon (2000) ArticleTitleTissue-/stage-dependent expression of a cloned Bombyx mandarina QM homologue. Biomol Eng 16 211–215 Occurrence Handle10.1016/S1389-0344(00)00056-3 Occurrence Handle1:CAS:528:DC%2BD3cXltVCrsrk%3D Occurrence Handle10894116
I. Imafuku T. Masaki M. Waragai S. Takeuchi M. Kawabata S.-i. Hirai S. Ohno L.E. Nee C.F. Lippa I. Kanazawa M. Imagawa H. Okazawa (1999) ArticleTitlePresenilin 1 suppresses the function of c-Jun homodimers via interaction with QM/Jif-1. J Cell Biol 147 121–133 Occurrence Handle10.1083/jcb.147.1.121 Occurrence Handle1:CAS:528:DyaK1MXmsVCltbw%3D Occurrence Handle10508860
H. Inada J. Mukai S. Matsushima T. Tanaka (1997) ArticleTitleQM is a novel zinc-binding transcription regulatory protein: its binding to c-Jun is regulated by zinc ions and phosphorylation by protein kinase C. Biochem Biophys Res Commun 230 331–334 Occurrence Handle10.1006/bbrc.1996.5955 Occurrence Handle1:CAS:528:DyaK2sXlsVartw%3D%3D Occurrence Handle9016777
T. Karl K. Onder R. Kodzius A. Pichova H. Wimmer A. Thur H. Hundsberger M. Loffler T. Klade A. Beyer M. Breitenbach L. Koller (1999) ArticleTitleGRC5 and NMD3 function in translational control of gene expression and interact genetically. Curr Genet 34 419–429
H.T. Koller T. Klade A. Ellinger M. Breitenbach (1996) ArticleTitleThe yeast growth control gene GRC5 is highly homologous to the mammalian putative tumor suppressor gene QM. Yeast 12 53–65 Occurrence Handle10.1002/(SICI)1097-0061(199601)12:1<53::AID-YEA886>3.0.CO;2-M Occurrence Handle1:CAS:528:DyaK28XnsFyjsg%3D%3D Occurrence Handle8789260
T.M. Loftus Y.H. Nguyen E.J. Stanbridge (1997) ArticleTitleThe QM protein associates with ribosomes in the rough endoplasmic reticulum. Biochemistry 36 8224–8230 Occurrence Handle10.1021/bi970288d Occurrence Handle1:CAS:528:DyaK2sXjvFCgsLo%3D Occurrence Handle9204867
F.S. Monteclaro P.K. Vogt (1993) ArticleTitleA Jun-binding protein related to a putative tumor suppressor. Proc Natl Acad Sci U S A 90 6726–6730 Occurrence Handle1:CAS:528:DyaK3sXlvFOisLw%3D Occurrence Handle8341691
H.S. Oh H. Kwon S.K. Sun C.H. Yang (2002) ArticleTitleQM, a putative tumor suppressor, regulates proto-oncogene c-Yes. J Biol Chem 277 36489–36498 Occurrence Handle10.1074/jbc.M201859200 Occurrence Handle1:CAS:528:DC%2BD38XnsVCjtbk%3D Occurrence Handle12138090
N. Saitou M. Nei (1987) ArticleTitleThe neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4 406–425 Occurrence Handle1:STN:280:BieC1cbgtVY%3D Occurrence Handle3447015
X. Shen A.M. Belcher P.K. Hansma G.D. Stucky D.E. Morse (1997) ArticleTitleMolecular cloning and characterization of Lustrin A, a matrix protein from shell and pearl nacre of Haliotis rufescens. J Biol Chem 272 32472–32481 Occurrence Handle1:CAS:528:DyaK1cXislWrtg%3D%3D Occurrence Handle9405458
S.M. Thomas J.S. Brugge (1997) ArticleTitleCellular functions regulated by Src family kinases. Annu Rev Cell Dev Biol 13 513–609 Occurrence Handle10.1146/annurev.cellbio.13.1.513 Occurrence Handle1:CAS:528:DyaK1cXisFSrsQ%3D%3D Occurrence Handle9442882
J.D. Thompson T.J. Gibson F. Plewniak F. Jeanmougin D.G. Higgins (1997) ArticleTitleThe CLUSTAL X Windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 24 4876–4882 Occurrence Handle10.1093/nar/25.24.4876
T. Tron M. Yang F.A. Dick M.E. Schmitt B.L. Trumpower (1995) ArticleTitle QSR1, an essential yeast gene with a genetic relationship to a subunit of the mitochondrial cytochrome bc 1 complex, is homologous to a gene implicated in eucaryotic cell differentiation. J Biol Chem 270 9961–9970 Occurrence Handle10.1074/jbc.270.17.9961 Occurrence Handle1:CAS:528:DyaK2MXltlWnsr8%3D Occurrence Handle7730379
I.G. Wool (1996) ArticleTitleExtraribosomal functions of ribosomal proteins. Trends Biochem Sci 21 164–165 Occurrence Handle10.1016/0968-0004(96)20011-8 Occurrence Handle1:CAS:528:DyaK28XivFentbk%3D Occurrence Handle8871397
Acknowledgements
We are grateful to Dr. Xiaozhuo Chen and Dr. Yunsheng Chen from Edison Biotechnology Institute of Ohio University for their technical help. This work was financially supported by the National High Technology Research and Development Program of China (2001AA621140) and the National Natural Science Foundation of China (30170723).
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Zhang, Y., Huang, J., Meng, Q. et al. Molecular Cloning and Expression of a Pearl Oyster (Pinctada fucata) Homologue of Mammalian Putative Tumor Suppressor QM . Mar. Biotechnol. 6, 8–16 (2004). https://doi.org/10.1007/s10126-002-0120-z
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10126-002-0120-z