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Temporal Transcription of Two Antennapedia Class Homeobox Genes in the Marine Copepod Calanus helgolandicus

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Abstract

DNA sequence information has been obtained for 2 genes implicated in playing critical roles in copepod development. The first (Cal-antp) was obtained by screening a Calanus helgolandicus genomic library. It shows homology to Antennapedia homeobox genes from a number of organisms including the centipede (76.8% over 250 bp) and the brine shrimp (75.5% over 220 bp). A second sequence (Hox 12) was obtained through reverse transcription polymerase chain reaction (PCR) of a solid-phase cDNA library constructed from Calanus eggs, and subsequent inverse PCR using a genomic DNA template. The Hox 12 sequence is homologous to the Antennapedia class of genes and shares homology with the chicken HoxB3 gene (71% over 187 bp) and with the fruit fly Sex Combs Reduced (Scr) homeotic gene (66% identity over 223 bp). Using sequence data from Cal-Antp and Hox 12, specific primers were designed to investigate the temporal expression of these genes by PCR analysis of 10 stage-specific cDNA libraries.

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Acknowledgements

This work was funded by the NERC Thematic Programme “Marine Productivity” and benefited from the use of the SEQNET facility. All experiments comply with the current laws of the United Kingdom.

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  1. Plymouth Marine Laboratory, Prospect Place, West Hoe, Plymouth, Devon PL1 3DH, U.K.

    Penelope K. Lindeque & Gary R. Smerdon

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  1. Penelope K. Lindeque
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Correspondence to Penelope K. Lindeque.

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Lindeque, P.K., Smerdon, G.R. Temporal Transcription of Two Antennapedia Class Homeobox Genes in the Marine Copepod Calanus helgolandicus . Mar. Biotechnol. 5, 604–615 (2003). https://doi.org/10.1007/s10126-002-0119-5

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