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Molecular Cloning and Tissue-Specific, Developmental-Stage-Specific, and Hormonal Regulation of IGFBP3 Gene in Zebrafish

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Abstract

The biological functions of insulin-like growth factor (IGF) I and II are modulated by a family of IGF-binding proteins (IGFBPs) in complex IGF-dependent and IGF-independent pathways. For further understanding of the actions of IGFs, some of these binding proteins have been cloned and characterized. We report the molecular cloning of IGFBP-3 cDNA for zebrafish. The tissue-specific and developmental stage-specific expression of IGFBP-3 and the hormonal regulation of its expression have also been determined by comparative reverse transcription polymerase chain reaction. Zebrafish IGFBP-3 cDNA contains an open reading frame of 879 bp, encoding a polypeptide of 293 amino acid residues. Results of this analysis revealed high levels of IGFBP-3 messenger RNA in ovary and fin tissue. Expression of IGFBP-3 mRNA was throughout the entire embryonic development, with the highest level of expression observed at 36 hours after the onset of development. Elevated levels of expression of IGFBP-3 were observed 24 hours after injection with IGF-I and 48 hours with IGF-II or insulin. These results suggest that the expression of IGFBP3 gene might be modulated by IGF-I, IGF-II, and insulin.

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Acknowledgements

This work was supported by a grant from the National Science Council of Taiwan (NSC91-2313-B-020-035) to J.L.W. The nucleotide sequence reported in this paper has been submitted to EMBL with accession number AJ299410 (IGFBP-3).

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Authors and Affiliations

  1. Institute of Zoology, Academia Sinica, Nankang, Taipei, Taiwan, R.O.C.

    Jyh-Yih Chen, Cheng-Wen Liu, Cho-Fat Hui & Jen-Leih Wu

  2. Department of Chemical Engineering, Southern Taiwan University of Technology, 1 Nan-Tai St. Yung-Kang City, Tainan Hsien, Taiwan, R.O.C.

    Jian-Chyi Chen

  3. Institute of Agricultural Biotechnology, Dayeh University, 112 Shan Jiau Road, Dah Tsuen, Chang Hwa, Taiwan, R.O.C.

    Wei-Tung Huang

  4. Department of Molecular and Cell Biology, University of Connecticut, 91 North Eagleville Road, U-3125, Storrs, CT 06269-3125, USA

    Thomas T. Chen

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  1. Jyh-Yih Chen
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Correspondence to Jen-Leih Wu.

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Chen, JY., Chen, JC., Huang, WT. et al. Molecular Cloning and Tissue-Specific, Developmental-Stage-Specific, and Hormonal Regulation of IGFBP3 Gene in Zebrafish . Mar. Biotechnol. 6, 1–7 (2004). https://doi.org/10.1007/s10126-002-0115-9

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  • DOI: https://doi.org/10.1007/s10126-002-0115-9

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