Skip to main content
SpringerLink
Log in

Grouping Newly Isolated Docosahexaenoic Acid-Producing Thraustochytrids Based on Their Polyunsaturated Fatty Acid Profiles and Comparative Analysis of 18S rRNA Genes

  • Original Contributions
  • Published:
Marine Biotechnology Aims and scope Submit manuscript

Abstract

Seven strains of marine microbes producing a significant amount of docosahexaenoic acid (DHA; C22:6, n-3) were screened from seawater collected in coastal areas of Japan and Fiji. They accumulate their respective intermediate fatty acids in addition to DHA. There are 5 kinds of polyunsaturated fatty acid (PUFA) profiles which can be described as (1) DHA/docosapentaenoic acid (DPA; C22:5, n-6), (2) DHA/DPA/eicosapentaenoic acid (EPA; C20:5, n-3), (3) DHA/EPA, (4) DHA/DPA/EPA/arachidonic acid (AA; C20:4, n-6), and (5) DHA/DPA/EPA/AA/docosatetraenoic acid (C22:4, n-6). These isolates are proved to be new thraustochytrids by their specific insertion sequences in the 18S rRNA genes. The phylogenetic tree constructed by molecular analysis of 18S rRNA genes from the isolates and typical thraustochytrids shows that strains with the same PUFA profile form each monophyletic cluster. These results suggest that the C20-22 PUFA profile may be applicable as an effective characteristic for grouping thraustochytrids.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Author information

Authors and Affiliations

  1. Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, Higashi-Hiroshima 739-8530, Japan

    Jianzhong Huang, Tsunehiro Aki, Seiji Kawamoto, Seiko Shigeta, Kazuhisa Ono & Osamu Suzuki

  2. Biological Resources Laboratory, National Institute of Advanced Industrial Science and Technology (AIST), Ibaraki 305-8566, Japan

    Toshihiro Yokochi & Toro Nakahara

  3. Department of Biology, Faculty of Science and Engineering, Konan University, Kobe 658-8501, Japan

    Daiske Honda

Authors
  1. Jianzhong Huang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Tsunehiro Aki
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Toshihiro Yokochi
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Toro Nakahara
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Daiske Honda
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Seiji Kawamoto
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Seiko Shigeta
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Kazuhisa Ono
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Osamu Suzuki
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Tsunehiro Aki.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Huang, J., Aki, T., Yokochi, T. et al. Grouping Newly Isolated Docosahexaenoic Acid-Producing Thraustochytrids Based on Their Polyunsaturated Fatty Acid Profiles and Comparative Analysis of 18S rRNA Genes. Mar. Biotechnol. 5, 450–457 (2003). https://doi.org/10.1007/s10126-002-0110-1

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10126-002-0110-1

Keywords

Search

Navigation