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Grouping Newly Isolated Docosahexaenoic Acid-Producing Thraustochytrids Based on Their Polyunsaturated Fatty Acid Profiles and Comparative Analysis of 18S rRNA Genes

Marine Biotechnology volume 5, pages 450–457 (2003)Cite this article

Abstract

Seven strains of marine microbes producing a significant amount of docosahexaenoic acid (DHA; C22:6, n-3) were screened from seawater collected in coastal areas of Japan and Fiji. They accumulate their respective intermediate fatty acids in addition to DHA. There are 5 kinds of polyunsaturated fatty acid (PUFA) profiles which can be described as (1) DHA/docosapentaenoic acid (DPA; C22:5, n-6), (2) DHA/DPA/eicosapentaenoic acid (EPA; C20:5, n-3), (3) DHA/EPA, (4) DHA/DPA/EPA/arachidonic acid (AA; C20:4, n-6), and (5) DHA/DPA/EPA/AA/docosatetraenoic acid (C22:4, n-6). These isolates are proved to be new thraustochytrids by their specific insertion sequences in the 18S rRNA genes. The phylogenetic tree constructed by molecular analysis of 18S rRNA genes from the isolates and typical thraustochytrids shows that strains with the same PUFA profile form each monophyletic cluster. These results suggest that the C20-22 PUFA profile may be applicable as an effective characteristic for grouping thraustochytrids.

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Authors and Affiliations

  1. Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, Higashi-Hiroshima 739-8530, Japan

    Jianzhong Huang, Tsunehiro Aki, Seiji Kawamoto, Seiko Shigeta, Kazuhisa Ono & Osamu Suzuki

  2. Biological Resources Laboratory, National Institute of Advanced Industrial Science and Technology (AIST), Ibaraki 305-8566, Japan

    Toshihiro Yokochi & Toro Nakahara

  3. Department of Biology, Faculty of Science and Engineering, Konan University, Kobe 658-8501, Japan

    Daiske Honda

Authors
  1. Jianzhong Huang
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  2. Tsunehiro Aki
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  3. Toshihiro Yokochi
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  4. Toro Nakahara
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  5. Daiske Honda
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  6. Seiji Kawamoto
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  7. Seiko Shigeta
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  8. Kazuhisa Ono
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  9. Osamu Suzuki
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Corresponding author

Correspondence to Tsunehiro Aki.

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Huang, J., Aki, T., Yokochi, T. et al. Grouping Newly Isolated Docosahexaenoic Acid-Producing Thraustochytrids Based on Their Polyunsaturated Fatty Acid Profiles and Comparative Analysis of 18S rRNA Genes. Mar. Biotechnol. 5, 450–457 (2003). https://doi.org/10.1007/s10126-002-0110-1

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  • DOI: https://doi.org/10.1007/s10126-002-0110-1

Keywords

  • 18S rRNA gene
  • docosahexaenoic acid
  • molecular phylogeny
  • polyunsaturated fatty acids
  • Schizochytrium
  • Thraustochytrium