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Genetic Diversity and Molecular Markers of the Tropical Abalone (Haliotis asinina) in Thailand

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Abstract

Genetic diversity of abalone in Thailand, Haliotis asinina, H. ovina, and H. varia, was analyzed by polymerase chain reaction (PCR) of 18S and 16S rDNAs, with randomly amplified polymorphic DNA (RAPD) and restriction fragment length polymorphism (RFLP). Species-specific RAPD markers were found in each abalone species. Restriction analysis of 18S (nuclear) ribosomal DNA with Alu I, Taq I, and Hae III and 16S (mitochondrial) rDNA with Bam HI, Eco RI, Hae III, and Alu I gave 12 and 13 digestion patterns, respectively. A total of 49 composite haplotypes were found. A dendogram obtained by the unweighted pair-group method with arithmetic mean, constructed from divergence between pairs of composite haplotypes, revealed reproductively isolated gene pools of these abalone and indicated that H. asinina and H. ovina are genetically closer than H. varia. When H. varia was discovered owing to small sample sizes, geographic heterogeneity analysis and F ST estimate indicated clear genetic differentiation between H. ovina originating from the Andaman Sea (west) and the Gulf of Thailand (east, P < 0.0001), whereas partial differentiation was observed between the Philippines and the remaining H. asinina samples (P < 0.0021). The amplified 16S rDNAs of individuals representing composite haplotypes found in this study were cloned and sequenced. A neighbor-joining tree constructed from sequence divergence of 16S rDNA accurately allocated those sequences according to species origins of abalone. Species-specific PCR based on 16S rDNA polymorphism was successfully developed in H. asinina and H. varia but not in H. ovina.

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References

  • J.C. Avise (1994) Molecular Markers, Natural History and Evolution. Chapman and Hall London, U.K.

    Google Scholar 

  • L.D. Brown (1995) ArticleTitleGenetic evidence for hybridisation between Haliotis rubra and H. laevigata. Mar Biol 123 89–93.

    Google Scholar 

  • W.J. Dower J.F. Miller C.W. Ragsdale (1988) ArticleTitleHigh efficiency transformation of E. coli by high voltage electroporation. Nucleic Acids Res 16 6127–6145. Occurrence Handle1:CAS:528:DyaL1cXltVGrtLw%3D Occurrence Handle3041370

    CAS  PubMed  Google Scholar 

  • J. Felsenstein (1993) PHYLIP (Phylogenetic Inference Package) Version 3.5c. Department of Genetics, University of Washington Seattle

    Google Scholar 

  • D.L. Geiger (1998) ArticleTitleRecent genera and species of the family Haliotidae Rafinesque, 1815 (Gastropoda: Vetigastropoda). Nautilus 111 85–116.

    Google Scholar 

  • D.E. Hamm R.S. Burton (2000) ArticleTitlePopulation genetics of black abalone, Haliotis cracheradii, along the central California coast. J Exp Mar Biol Ecol 254 235–247. Occurrence Handle10.1016/S0022-0981(00)00283-5 Occurrence Handle1:CAS:528:DC%2BD3cXptFSqtrc%3D Occurrence Handle11077063

    Article  CAS  PubMed  Google Scholar 

  • D.D. Heath O.K. Iwama R.H. Delvin (1993) ArticleTitlePCR primed with VNTR core sequences yields species-specific patterns and hypervariable probes. Nucleic Acids Res 21 5782–5785.

    Google Scholar 

  • B.X. Huang R. Peakall P.J. Hanna (2000) ArticleTitleAnalysis of genetic structure of blacklip abalone (Haliotis rabra) populations using RAPD, minisatellite and microsatellite markers. Mar Biol 136 207–216.

    Google Scholar 

  • P. Jarayabhand N. Paphavasit (1996) ArticleTitleA review of the culture of tropical abalone with special reference to Thailand. Aquaculture 140 159–168.

    Google Scholar 

  • P. Jarayabhand R. Yom-La A. Popongviwat (1998) ArticleTitleKaryotypes of marine molluscs in the family Haliotidae found in Thailand. J Shellfish Res 17 761–764.

    Google Scholar 

  • M. Kimura (1980) ArticleTitleA simple method for estimating evolutionary rate of base substitutions through comparative studies of nucleotide sequences. J Mol Biol 16 111–120. Occurrence Handle1:CAS:528:DyaL3MXmtFSktg%3D%3D

    CAS  Google Scholar 

  • S. Klinbunga S. Sodsuk D. Penman B.J. McAndrew (1996) ArticleTitleAn improved protocol for total DNA isolation and visualization of mtDNA RFLP(s) in tiger prawn, Penaeus monodon. Thai J Aquat Sci 3 36–41.

    Google Scholar 

  • S. Klinbunga A. Boonyapakdee B. Pratoomchat (2000) ArticleTitleGenetic diversity and species-diagnostic markers of mud crabs (genus Scylla) in eastern Thailand determined by RAPD analysis. Mar Biotechnol 2 180–187.

    Google Scholar 

  • S. Klinbunga D. Siludjai W. Wudthijinda A. Tassanakajon P. Jarayabhand P. Menasaveta (2001) ArticleTitleGenetic heterogeneity of the giant tiger shirmp (Penaeus monodon) in Thailand revealed by RAPD and mitochondrial DNA RFLP analyses. Mar Biotechnol 3 428–438.

    Google Scholar 

  • S. Klinbunga N. Khamnamtong A. Tassanakajon N. Puanglarp P. Jarayabhand W. Yoosukh (2002) ArticleTitleMolecular genetic identification tools for three commercially cultured oysters (Crassostrea belcheri, Crassostrea iredalei, and Saccostrea cucullata) in Thailand. Mar Biotechnol 5 27–36.

    Google Scholar 

  • Y.H. Lee V.D. Vacquier (1995) ArticleTitleEvolution and systematics in Haliotidae (Mollusca: Gastropoda): inferences from DNA sequences of sperm lysin. Mar Biol 124 267–278.

    Google Scholar 

  • T. Maniatis E.F. Frisch J. Sambrook (1982) Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press Cold Spring Harbor, N.Y.

    Google Scholar 

  • McElroy, D., Moran, P., Bermingham, E., and Kornfield, I. (1991). REAP; The Restriction Enzyme Analyses Package, Version 4.0

  • A.E. Muchmore G.W. Moy W.J. Swanson V.D. Vacquier (1998) ArticleTitleDirect sequencing of genomic DNA for characterization of a satellite DNA in five species of Eastern Pacific abalone. Mol Mar Biol Biotechnol 7 1–6.

    Google Scholar 

  • T. Naganuma K. Hisadome K. Shiraishi H. Kojima (1998) ArticleTitleMolecular distinction of two resemblant abalones, Haliotis discus discus and Haliotis discus hannai by 18S rDNA sequences. J Mar Biotechnol 6 59–61.

    Google Scholar 

  • A. Nateewathana S. Bussarawit (1988) ArticleTitleAbundance and distribution of abalones along the Andaman Sea coast of Thailand. Kasetsart J (Natural Sci) 22 8–15.

    Google Scholar 

  • M. Nei (1987) Molecular Evolutionary Genetics. Columbia University Press New York, N.Y.

    Google Scholar 

  • M. Nei W. Li (1979) ArticleTitleMathematical model for studying genetic variation in terms of restriction endonucleases. Proc Natl Acad Sci USA 76 5269–5273. Occurrence Handle1:CAS:528:DyaL3cXitVWn Occurrence Handle291943

    CAS  PubMed  Google Scholar 

  • M. Nei F. Tajima (1981) ArticleTitleDNA polymorphism detectable by restriction endonucleases. Genetics 97 145–163. Occurrence Handle1:CAS:528:DyaL3MXksFKmu7c%3D Occurrence Handle6266912

    CAS  PubMed  Google Scholar 

  • S.R. Palumbi A. Martin S. Pomano W.O. McMillan G. Grabowski (1991) The Simple Fool’s Guide to PCR, Version 2. Zoology Department University of Hawaii.

    Google Scholar 

  • M. Raymond F. Rousset (1995) ArticleTitleGENEPOP (Version 1.2): population genetic software for exact tests and ecumenicism. J Hered 86 248–249.

    Google Scholar 

  • W.R. Rice (1989) ArticleTitleAnalyzing tables for statistical tests. Evolution 41 223–225.

    Google Scholar 

  • D.A. Roff P. Bentzen (1989) ArticleTitleThe statistical analysis of mitochondrial DNA polymorphisms: χ2 and the problems of small samples. Mol Biol Evol 6 539–545. Occurrence Handle1:CAS:528:DyaL1MXlslSmt7c%3D Occurrence Handle2677600

    CAS  PubMed  Google Scholar 

  • N. Saitou M. Nei (1987) ArticleTitleThe neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4 406–425. Occurrence Handle1:STN:280:BieC1cbgtVY%3D Occurrence Handle3447015

    CAS  PubMed  Google Scholar 

  • S.A. Shepherd D. Lowe D. Partington (1992) ArticleTitleStudies of southern Australian abalone (genus Haliotis), 13: larval dispersal and recruitment. J Exp Mar Biol Ecol 164 247–260.

    Google Scholar 

  • T. Singhagraiwan M. Doi (1993) Seed production and culture of a tropical abalone, Haliotis asinina Linne’. Department of Fisheries, Ministry of Agriculture and Cooperatives Thailand.

    Google Scholar 

  • M.P. Small R.W. Chapman (1997) ArticleTitleIntraspecific variation in the 16S ribosomal gene of Crassostrea virginica. Mol Mar Biol Biotechnol 6 189–196.

    Google Scholar 

  • P.H.A. Sneath R.R. Sokal (1973) Numerical Taxonomy. W.H. Freeman and Co San Franciso, Calif.

    Google Scholar 

  • N.A. Sweijd R.C.K. Bowie A.L. Lopata A.M. Marinaki E. Harley P.A. Cook (1998) ArticleTitleA PCR technique for forensic, species-level identification of abalone tissue. J Shellfish Res 17 889–895.

    Google Scholar 

  • J.D. Thompson D.G. Higgin T.J. Gibson (1994) ArticleTitleCLUSTAL W: improving the sensitivity of progressive multiple sequence weighting, position-specific penalties and weight metric choices. Nucleic Acids Res 22 4673–4680. Occurrence Handle1:CAS:528:DyaK2MXitlSgu74%3D Occurrence Handle7984417

    CAS  PubMed  Google Scholar 

  • Tookwinas, S., Leknim, V., Donyadol, Y., Predalampabut, Y., and Paengmark, P. (1986). A survey of species and distribution of abalone (Haliotis spp) in Surat Thani, Nakhon Si Thammarat and Songkla. Technical Report No. 1/1986, NICA.

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Acknowledgements

We thank the National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), and Department of Biochemistry, Faculty of Science, Chulalongkorn University, for providing facilities required by our experiments. This work is supported by the Thailand Research Funds (TRF) project 4320015 awarded to S.K. and A.T. and by the JSPS to S.K.

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Authors and Affiliations

  1. Marine Biotechnology Research Unit, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency, 113 Paholyothin Rd., Klong 1, Klong Luang, Patthumthani 12120, Thailand

    S. Klinbunga, N. Khamnamtong, N. Puanglarp & P. Menasveta

  2. Program of Biotechnology, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand

    P. Pripue

  3. Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand

    A. Tassanakajon

  4. Aquatic Resources Research Institute, Chulalongkorn University, Bangkok 10330, Thailand

    P. Jarayabhand

  5. Department of Marine Science, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand

    P. Jarayabhand & P. Menasveta

  6. Department of Aquatic Biosciences, Tokyo University of Fisheries, Konan 4-5-7, Tokyo 108-8477, Japan

    I. Hirono & T. Aoki

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  1. S. Klinbunga
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  2. P. Pripue
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  3. N. Khamnamtong
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  4. N. Puanglarp
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  5. A. Tassanakajon
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  6. P. Jarayabhand
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  7. I. Hirono
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  8. T. Aoki
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  9. P. Menasveta
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Correspondence to S. Klinbunga.

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Klinbunga, S., Pripue, P., Khamnamtong, N. et al. Genetic Diversity and Molecular Markers of the Tropical Abalone (Haliotis asinina) in Thailand . Mar. Biotechnol. 5, 505–517 (2003). https://doi.org/10.1007/s10126-002-0108-8

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  • DOI: https://doi.org/10.1007/s10126-002-0108-8

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