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Photoproduction of Hydrogen by the Marine Heterocystous Cyanobacterium Anabaena Species TU37-1 Under a Nitrogen Atmosphere

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Abstract

Hydrogen production rates by Anabaena sp. strain TU37-1 obtained after an initial 1-day incubation period were approximately 70 to 80 and 3 to 9 µmol (mg chl)−1 h−1 under argon and nitrogen atmospheres, respectively. Hydrogen production under argon was not enhanced by addition of carbon dioxide, but was enhanced to some extent under nitrogen by increasing the initial carbon dioxide concentration. Rates of hydrogen and oxygen production during the initial 7-hour period were 15 and 220 µmol (mg chl)−1 h−1, respectively, in vessels with 18.5% initial carbon dioxide. Hydrogen production under nitrogen was enhanced by addition of carbon monoxide (1%). The rate obtained from the initial 1-day incubation period was about 40 µmol (mg chl)−1 h−1, which corresponded to about 60% of that under argon. On the basis of these observations, a possible strategy for hydrogen production by nitrogen-fixing cyanobacteria under nitrogen in the presence of carbon monoxide is indicated.

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Acknowledgements

This work was carried out as a part of the research project organized by Dr. Iwao Watanabe (Professor of Mie University, Japan). One of his suggestions, that nitrogenase with minimum activities for all substrates except for proton could be an ideal enzyme for hydrogen production, was extended using strain TU37-1. I would like to express sincere gratitude to Dr. Watanabe.

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  1. Department of Marine Science, School of Marine Science and Technology, Tokai University, 3-20-1 Orido, Shimizu, Shizuoka 424-8610, Japan

    Shuzo Kumazawa

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  1. Shuzo Kumazawa
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Correspondence to Shuzo Kumazawa.

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Kumazawa, S. Photoproduction of Hydrogen by the Marine Heterocystous Cyanobacterium Anabaena Species TU37-1 Under a Nitrogen Atmosphere . Mar. Biotechnol. 5, 222–226 (2003). https://doi.org/10.1007/s10126-002-0106-x

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  • DOI: https://doi.org/10.1007/s10126-002-0106-x

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