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Antimicrobial Activities and Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry of Bacillus Isolates from the Marine Sponge Aplysina aerophoba

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Abstract

The aim of this study was to isolate bacteria that are resistant to the strong antimicrobial metabolites characteristic of Aplysina aerophoba. For this purpose, bacterial isolation was performed on agar plates to which sponge tissue extract had been added. Following screening for antifungal and antimicrobial activities, 5 strains were chosen for more detailed analyses. 16S ribosomal DNA sequencing revealed that all isolates belonged to the genus Bacillus, specifically B. subtilis and B. pumilus. Using a combination of matrix-assisted laser desorption/ ionization mass spectrometry typing of whole cells and antimicrobial bioassays against selected reference strains, the bioactive metabolites were identified as lipopeptides.

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Authors and Affiliations

  1. Institut für Molekulare Infektionsbiologie, Universität Würzburg, Röntgenring 11, D-97070 Würzburg, Germany

    Christian T. Pabel, Jörg Hacker & Ute Hentschel

  2. Institut für Chemie, Arbeitsgruppe Biochemie und Molekulare Biologie, Technische Universität Berlin, Franklinstrabe 29, D-10587 Berlin, Germany

    Joachim Vater & Christopher Wilde

  3. Institut für Biochemie, Freie Universität Berlin, Thielallee 63, D-14195 Berlin, Germany

    Peter Franke

  4. Institut für Pflanzengenetik und Kulturpflanzenforschung, Abteilung Molekulare Genetik, Corrensstrabe 3, D-06466 Gatersleben, Germany

    Jürgen Hofemeister & Barbara Adler

  5. Institut für Organische Chemie, Universität Würzburg, Am Hubland, D-97074 Würzburg, Germany

    Gerhard Bringmann

Authors
  1. Christian T. Pabel
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  2. Joachim Vater
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  3. Christopher Wilde
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  4. Peter Franke
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  5. Jürgen Hofemeister
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  6. Barbara Adler
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  7. Gerhard Bringmann
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  8. Jörg Hacker
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  9. Ute Hentschel
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Corresponding author

Correspondence to Ute Hentschel.

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Pabel, C., Vater, J., Wilde, C. et al. Antimicrobial Activities and Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry of Bacillus Isolates from the Marine Sponge Aplysina aerophoba . Mar. Biotechnol. 5, 424–434 (2003). https://doi.org/10.1007/s10126-002-0088-8

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  • DOI: https://doi.org/10.1007/s10126-002-0088-8

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