Marine Biotechnology

, Volume 5, Issue 2, pp 130–140 | Cite as

Phylogenetic Identification of Symbiotic Dinoflagellates via Length Heteroplasmy in Domain V of Chloroplast Large Subunit (cp23S)—Ribosomal DNA Sequences

  • Scott R. Santos
  • Carla Gutierrez-Rodriguez
  • Mary Alice Coffroth
Article

Abstract

A protocol that takes advantage of length heteroplasmy in domain V of chloroplast large subunit (cp23S)–ribosomal DNA to identify members of the symbiotic dinoflagellate genus Symbiodinium is presented. This protocol is highly specific for Symbiodinium, can provide intercladal and intracladal identification of a particular Symbiodinium isolate, and can detect multiple Symbiodinium chloroplast genotypes simultaneously in the same isolate, making his technique attractive for a variety of research questions. We used this technique to characterize variation among Symbiodinium populations associated with a range of phylogenetically diverse and geographically discrete hosts. We also examined symbiont variation within a single host, the Caribbean gorgonian Pseudopterogorgia elisabethae, from 9 sites in the Bahamas, and we report a previously undocumented level of symbiont specificity for particular members of Symbiodinium clade B in this gorgonian.

Keywords

chloroplast large subunit ribosomal genes dinoflagellate length heteroplasmy Symbiodinium zooxanthellae 

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Copyright information

© Springer-Verlag New York Inc. 2003

Authors and Affiliations

  • Scott R. Santos
    • 1
  • Carla Gutierrez-Rodriguez
    • 1
  • Mary Alice Coffroth
    • 1
  1. 1.Department of Biological ScienceState University of New York at Buffalo, Buffalo, NY 14260-1300USA

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