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Methoprene Photolytic Compounds Disrupt Zebrafish Development, Producing Phenocopies of Mutants in the Sonic Hedgehog Signaling Pathway

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Abstract

Environmental chemicals have been proposed to impact endocrine or retinoid pathways, causing developmental abnormalities in humans and other vertebrates. Presented evidence shows that exposure of zebrafish embryos to sunlight-induced photolytic products of the pesticide methoprene results in developmental defects in the head, heart, pectoral fins, and somites, and in spinal motor and optic nerve axons. Exposed embryos are phenocopies of zebrafish you-type mutants and, as in the mutant sonic-you, show underexpression of the signaling protein sonic hedgehog. Reduced expression of sonic hedgehog is also displayed in embryos treated with the retinoic acid synthesis inhibitor citral. This study identifies citral-related compounds as embryonic signaling disruptors of potential environmental concern.

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Acknowledgements

We thank the laboratory of Corey Goodman for the engrailed antibody; R. Markwald and the Department of Cell Biology and Anatomy of MUSC for support; and S. Argraves, J. Barth, P. Fleming, S. Hammad, C. Knaack, A. Ringwood, W. Twal, and M. Vandersea for comments on the manuscript. Funding was provided by National Institutes of Health Program Project Grant to R.A.M. and by Christopher Newport University and the College of Charleston to G.D.S. Special thanks to Scott Argraves for discussions, support, encouragement, and use of space and resources, and to Fred Holland of South Carolina Marine Resources Research Institute for initial discussions on environmental monitoring and developmental genes.

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  1. Department of Biology, Chemistry and Environmental Science, Christopher Newport University, 1 University Place, Newport News, VA 23606, USA

    Denice G. Smith

  2. 111 University Village, Clemson University, Clemson, SC 29632, USA

    Claudia Wilburn

  3. Department of Cell Biology and Anatomy, Medical University of South Carolina, 173 Ashley Avenue, P.O. Box 250508, Charleston, SC 29425, USA

    Robert A. McCarthy

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Correspondence to Robert A. McCarthy.

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Smith, D.G., Wilburn, C. & McCarthy, R.A. Methoprene Photolytic Compounds Disrupt Zebrafish Development, Producing Phenocopies of Mutants in the Sonic Hedgehog Signaling Pathway . Mar. Biotechnol. 5, 201–212 (2003). https://doi.org/10.1007/s10126-002-0062-5

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