Ontogeny of α-Amylase Gene Expression in Sea Bass Larvae (Lates calcarifer)

Abstract:

The enzymatic activities of α-amylase and its corresponding messenger RNA levels in developing sea bass (Lates calcarifer) larvae were studied from hatching until 27 days post hatching (dph). An increasing activity of amylase enzyme was measured until 5 dph, and the activity gradually decreased thereafter and reached a constant level by 12 dph. To achieve a better understanding of the molecular mechanisms underlying amylase expression, we have cloned and sequenced a 318-bp fragment of α-amylase complementary DNA. Based on this sequence, a real-time reverse transcriptase polymerase chain reaction technique to monitor the changes in the mRNA levels in the larvae was developed. A correlation between enzymatic activity and mRNA level of α-amylase could be demonstrated during the early development of sea bass larvae. This suggests that the changes in α-amylase are controlled at least at the transcriptional level during early larval development of sea bass.