Abstract
Pleurotus pulmonarius is a medium temperature edible mushroom, and its yield and quality are severely affected by high temperature. However, the molecular mechanism of Pleurotus pulmonarius response to heat stress remains unknown. In this study, transcriptome sequencing and analysis of Pleurotus pulmonarius mycelia under heat stress were performed, related differentially expressed genes (DEGs) were verified by fluorescence quantitative PCR (qPCR) and the reduced glutathione content was detected. 5906 DEGs, including 1086 upregulated and 4820 downregulated, were identified by RNA-Seq. GO analysis revealed that DEGs were mainly enriched in the pathways of Aminoacyl-tRNA biosynthesis, pyrimidine metabolism, arginine and proline metabolism, fructose and mannose metabolism, and glutathione metabolism. qPCR analysis showed that the expression of ggt decreased after heat stress treatment, while gst2 and gst3 increased. The glutathione content in mycelia after heat stress was significantly higher than that in the control group. These results suggest that glutathione metabolism may play an important role in the response to heat stress. Our study will provide a molecular-level perspective on fungal response to heat stress and a basis for research on fungal environmental response and molecular breeding.
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No datasets were generated or analysed during the current study.
Abbreviations
- DEGs:
-
Differentially expressed genes
- qPCR:
-
Quantitative real-time polymerase chain reaction
- GST:
-
Glutathione-S-transferase
- GSH:
-
Glutathione
- PDA:
-
Potato dextrose agar
- GO:
-
Gene ontology
- KEGG:
-
Kyoto Encyclopedia of Genes and Genomes
- GAPDH:
-
Glyceraldehyde-3-phosphate dehydrogenase
- ROS:
-
Reactive oxygen species
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The authors gratefully acknowledge the supports of the Westlake University, Jin Weiyuan.
Funding
This research has been funded by Zhejiang Major Science and Technology Projects of New Agricultural Varieties Breeding (2021C02073) and Project of Administration of Traditional Chinese Medicine of Zhejiang Province of China (2024ZF049).
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WW: conceptualization, project administration, writing—original draft. CG: resources, supervision, methodology, investigation, project administration, writing—review and editing. LN: conceptualization, supervision, writing—review and editing. LJ: methodology, resources. LL: methodology, data curation, formal analysis.
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Weike, W., Na, L., Jiayao, L. et al. Transcriptome analysis reveals key genes and pathways associated with heat stress in Pleurotus pulmonarius. Int Microbiol (2024). https://doi.org/10.1007/s10123-024-00536-4
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DOI: https://doi.org/10.1007/s10123-024-00536-4