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Epidemiological and molecular study of Providencia rettgeri outbreak at a university hospital during the COVID-19 reference center

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Abstract

In 2014, Brazil detected New Delhi metallo-β-lactamase (NDM)-producing Enterobacterales from a Providencia rettgeri isolate obtained through surveillance swabs in the Southern region. Subsequently, various species have reported several NDM enzymes. However, comprehensive data on the current epidemiology of NDM-producing P. rettgeri in Brazil remains limited. This study, aimed to provide a detailed characterization of the phenotypic, genotypic, and epidemiological profile of clinical isolates of P. rettgeri NDM. From April 2020 to December 2022, 18 carbapenem-resistant P. rettgeri strains, previously identified using Vitek2®, were isolated at the University Hospital of Londrina. Resistance and virulence genes were assessed through genetic analysis using ERIC PCR and NextSeq (Illumina) sequencing. Statistical analysis was conducted using SPSS version 2.0. Genomic analysis confirmed the presence of β-lactamase blaNDM-1 and blaOXA-1. All isolates showed the presence of the NDM encoding gene and genetic similarity above 90% between isolates. Clinical parameters of patients infected with P. rettgeri exhibited significant association with mechanical ventilation, prior use of carbapenems, and polymyxins. We also report a significant association between P. rettgeri infection and death outcome. This study characterizes NDM-1 metallo-β-lactmases isolates, among P. rettgeri isolates from patients at the University Hospital (HU), during the COVID-19 pandemic. The emergence of this novel resistance mechanism among P. rettgeri poses a significant challenge, limiting the therapeutic options for infections in our hospital.

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Data availability

The datasets generated during and/or analyzed during the current study are available from the corresponding author upon reasonable request.

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Acknowledgements

The authors acknowledge the Araucaria Foundation, the Research Support Facilities Center of the State University of São Paulo (CEFAP-USP), the Institute of Biomedical Sciences of the State University of São Paulo (ICB-USP), the Clinical Microbiology Laboratory of the University Hospital, and the Master’s Program of the Clinical and Laboratory Pathophysiology of the State University of Londrina.

Funding

Partial financial support was received from the Araucaria Foundation.

Author information

Authors and Affiliations

  1. Clinical and Laboratory Pathophysiology, Health Sciences Center, State University of Londrina, Londrina, Paraná, Brazil

    Julia Da Silva Pimenta

  2. Clinical and Laboratory of Microbiology, Health Sciences Center, State University of Londrina, Londrina, Paraná, Brazil

    Gerusa Luciana Gomes Magalhães

  3. Clinical and Laboratory Pathophysiology, Health Sciences Center, State University of Londrina, Londrina, Paraná, Brazil

    Joao Gabriel Material Soncini

  4. Department of Microbiology, Institute of Biomedical Sciences (IBS), University of São Paulo, São Paulo, São Paulo, Brazil

    Nilton Licopan

  5. Department of Pathology, Clinical and Toxicological Analyzes, Health Sciences Center, State University of Londrina, Londrina, Paraná, Brazil

    Eliana Carolina Vespero

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  1. Julia Da Silva Pimenta
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  2. Gerusa Luciana Gomes Magalhães
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  3. Joao Gabriel Material Soncini
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  4. Nilton Licopan
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  5. Eliana Carolina Vespero
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Contributions

J.S.P. and G.L.G.M. are responsible for the collection of samples and their processing. J.G.M.S., E.C.V., and N.L. are responsible for sequencing genomic and bioinformatics analyses. J.S.P. and E.C.V. are responsible for the construction of the text. All authors were responsible for reviewing the text.

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Correspondence to Julia Da Silva Pimenta.

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The study was approved by the Ethics and Research Committee of the State University of Londrina CAAE 28316819.0.0000.5231.

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The authors declare no competing interests.

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Da Silva Pimenta, J., Magalhães, G.L.G., Soncini, J.G.M. et al. Epidemiological and molecular study of Providencia rettgeri outbreak at a university hospital during the COVID-19 reference center. Int Microbiol (2024). https://doi.org/10.1007/s10123-024-00523-9

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  • DOI: https://doi.org/10.1007/s10123-024-00523-9

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