Abstract
In addition to the UPR pathway, yeast cells require components of the HOG pathway to respond to ER stress. In this work, we found that unphosphorylated Sln1 and Ssk1 are required to mount an appropriate response to Tn. We also found that the MAPKKKs Ssk2 participates in the Tn response, but its osmo-redundant protein Ssk22 does not. We also found that the Pbs2 docking sites for Ssk2 (RDS-I and KD) are partially dispensable when mutated separately; however, the prevention of Ssk2 binding to Pbs2, by the simultaneous mutation of RDS-I and KD, caused strong sensitivity to Tn. In agreement with the lack of Hog1 phosphorylation during Tn treatment, a moderate resistance to Tn is obtained when a Pbs2 version lacking its kinase activity is expressed; however, the presence of mutual Pbs2-Hog1 docking sites is essential for the Tn response. Finally, we detected that Tn induced a transcriptional activation of some components of the SLN1 branch. These results indicate that the Tn response requires a complex formed by the MAPK module and components of the SLN1 branch but not their canonical osmoregulatory activities.
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Acknowledgements
We thank Francesc Posas for the Sln1(TS) strain. GS-D is a PhD student of the Biochemical Sciences Program, UNAM. We acknowledge the technical support provided by Guadalupe Codiz and Minerva Mora from the Molecular Biology Unit; Gerardo Coello, Ana Ma. Escalante, Juan Barbosa, and Ivette Rosas from the Computing Unit; and Aurey Galván and Manuel Ortínez from the Maintenance Workshop. The microarray analysis was performed in the Microarray Unit (IFC, UNAM). We thank Lorena Chávez González, Simón Guzmán León, and Jorge Ramírez for technical assistance in the microarray determinations. We also acknowledge the technical assistance of Ma. Teresa Lara Ortíz.
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This work was supported in part by grants DGAPA-PAPIIT No. IN210519 and CONACyT No. CF-58550.
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MH-E, LK, GS-D, ED-M, UC-M, and AEO performed experiments. FT-Q, LO-L, and RC conceived the project. MH-E, LK, GS-D, ED-M, and RC designed experiments and analyzed the data. ED-M designed and performed the microarray analysis. FT-Q and RC supervised the project. MH-E and RC wrote the first draft of the manuscript. LO-L and RC wrote the final version of the manuscript with comments from all authors.
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Hernández-Elvira, M., Salas-Delgado, G., Kawasaki, L. et al. The yeast two-component SLN1 branch of the HOG pathway and the scaffolding activity of Pbs2 modulate the response to endoplasmic reticulum stress induced by tunicamycin. Int Microbiol 25, 639–647 (2022). https://doi.org/10.1007/s10123-022-00250-z
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DOI: https://doi.org/10.1007/s10123-022-00250-z