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Extraction and characterization of an exopolysaccharide from a marine bacterium

Abstract

The marine bacterial exopolysaccharides (EPS) have transfigured the biotech sector with their myriad applications and prospects. This work was carried out to characterize and analyze the functional and biochemical properties of an EPS (EPS-DR3A) produced by a marine bacterium, Pseudoalteromonas sp. YU16-DR3A. The bacterium was cultured in Zobell marine broth for the production of EPS. The extracted EPS designated as EPS-DR3A was composed of 69% carbohydrates and 7.6% proteins with a molecular weight of 20 kDa. FT-IR spectra showed the presence of different functional groups. The monosaccharide analysis performed using GC–MS showed the presence of fucose, erythrotetrose, ribose, and glucose as monomers. EPS-DR3A showed excellent emulsifying activity against the tested hydrocarbons and food oils with stable emulsions. Rheological analysis of EPS-DR3A revealed the pseudoplastic behavior. The EPS-DR3A displayed good thermal stability with a degradation temperature of 249 °C and a melting point at 322 °C. Further, it had the ability to scavenge DPPH and nitric oxide free radicals with good total antioxidant activity. The in vitro biocompatibility study of EPS-DR3A showed high degree of biocompatibility with human dermal fibroblast cells at the tested concentrations. Taken together, the findings such as thermostability, emulsifying activity, pseudoplasticity, antioxidant activity, and biocompatibility of EPS-DR3A make this biomolecule an important candidate for a wide range of biomedical applications.

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Acknowledgements

Authors would like to acknowledge Dr. Priyanka and Dr. Nagaraj for their valuable guidance and Prof. C.C. Young for gene sequencing analysis.

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B.E. Dhanya performed the experiments and drafted the manuscript. A. Prabhu was involved in the experiments pertaining to cell culture and revision of the manuscript. P.D. Rekha designed the experiments, analyzed the data, and critically revised the manuscript.

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Correspondence to Punchappady Devasya Rekha.

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The authors declare no competing interests.

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Dhanya, B.E., Prabhu, A. & Rekha, P.D. Extraction and characterization of an exopolysaccharide from a marine bacterium. Int Microbiol 25, 285–295 (2022). https://doi.org/10.1007/s10123-021-00216-7

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  • DOI: https://doi.org/10.1007/s10123-021-00216-7

Keywords

  • Pseudoalteromonas sp.
  • Marine exopolysaccharide
  • Thermostability
  • Emulsification
  • Rheology
  • Biocompatibility