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Enhancement of vincristine under in vitro culture of Catharanthus roseus supplemented with Alternaria sesami endophytic fungal extract as a biotic elicitor


Vincristine, one of the major vinca alkaloid of Catharanthus roseus (L.) G. Don. (Apocynaceae), was enhanced under in vitro callus culture of C. roseus using fungal extract of an endophyte Alternaria sesami isolated from the surface-sterilized root cuttings of C. roseus. Vindoline, a precursor molecule for vincristine production, was detected for the first time in the fungal endophyte A. sesami which was used as a biotic elicitor in this study to enhance vincristine content in the C. roseus callus. It was identified using high-performance liquid chromatography and mass spectroscopy techniques by matching retention time and mass data with reference molecule. Supplementing the heat sterilized A. sesami endophytic fungal culture extract into the callus culture medium of C. roseus resulted in the enhancement of vincristine content in C. roseus callus by 21.717% after 105-day culture.

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The first author is thankful to CSIR-UGC for providing a scholarship for carrying out this work. The author also acknowledges the efforts of DST for providing financial assistance through (DST-PURSE), Govt. of India. The author is grateful to the School of Chemical and Life Science and the Central Instrumentation Facility (CIF), Jamia Hamdard, for providing research facilities.

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Correspondence to Bibhu Prasad Panda.

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The data available in attached supplementary file includes comparison of plant growth hormone combinations for good callus initiation, effect of hormone concentration on callus growth, callus growth in 90-day culture, HPLC data for vindoline standard, standard plot for vindoline detection, standard plot for vincristine detection, aligned sequence of isolated fungus Alternaria sesami and biosynthetic pathway for vincristine production with precursor molecule vindoline.

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Birat, K., Siddiqi, T.O., Mir, S.R. et al. Enhancement of vincristine under in vitro culture of Catharanthus roseus supplemented with Alternaria sesami endophytic fungal extract as a biotic elicitor. Int Microbiol 25, 275–284 (2022).

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  • Vindoline
  • Vincristine
  • Catharanthus roseus
  • Alternaria sesami
  • Endophyte