Skip to main content

A pilot study to investigate the alteration of gut microbial profile in Dip2a knockout mice

Abstract

Accumulating evidence has pointed out that the gut-brain axis plays important roles in the etiology of autism spectrum disorder (ASD). Gut dysbiosis was reported in both ASD human patients and animal models. Dip2a was identified as a human ASD candidate gene. Deletion of Dip2a led to dendritic spine dysfunction and autistic-like behaviors in mice. To further investigate if Dip2a deletion leads to gut dysbiosis, we used 16S rDNA sequencing to study the gut microbiota in Dip2a KO mice. In both co-housed and separated breeding conditions, deletion of Dip2a could affect the gut microbiome composition. The probiotic bacteria, Lactobacillus and Bifidobacterium, became less abundant, while some potentially harmful bacteria, Alistipes, Lachnospiraceae_NK4A136_group, Clostridium, Desulfovibrio, and Enterorhabdus, became more abundant. We further found that probiotic treatment could help to reconstitute the gut microbiome composition in Dip2a KO mice. Altogether, these data showed DIP2A is required for the proper composition of gut microbiota, and the probiotics have potential roles in rectifying the gut microbiota in Dip2a KO mice.

This is a preview of subscription content, access via your institution.

Fig. 1
Fig. 2

Data availability

Please contact author for data requests.

References

Download references

Funding

This work was supported by the National Natural Science Foundation of China (31871040 and 32070809), the Jilin Science and Technology department (20191008009TC), and the Fundamental Research Funds for the Central Universities (2412019FZ025 and 2412019FZ027).The sponsors had no role in the design, execution, interpretation, or writing of the study.

Author information

Authors and Affiliations

Authors

Contributions

Conceptualization, X.H. and S.L.; methodology and validation, Y.Z., Y.Q., J.Y., and J.L; writing (original draft preparation), X.H. and Y.Z.; writing (review and editing), S.L. and X.H.; funding acquisition, X.H.

Corresponding authors

Correspondence to Shengnan Li or Xiaoxiao He.

Ethics declarations

Ethics approval and consent to participate

All mice used in this study were handled in accordance with the guidelines of the Institutional Animal Care and Use Committee of Northeast Normal University, China (NENU/IACUC, AP20151009).

Consent for publication

Not applicable

Competing interests

The authors declare no competing interests.

Additional information

Publisher's note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Supplementary Information

Rights and permissions

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Zhang, Y., Qu, Y., Yang, J. et al. A pilot study to investigate the alteration of gut microbial profile in Dip2a knockout mice. Int Microbiol 25, 267–274 (2022). https://doi.org/10.1007/s10123-021-00211-y

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10123-021-00211-y

Keywords

  • Autism spectrum disorder
  • Dip2a
  • Gut-brain axis
  • Gut microbiota
  • Probiotic
  • 16 s rDNA