Skip to main content
Log in

A walk-through MAPK structure and functionality with the 30-year-old yeast MAPK Slt2

  • Review
  • Published:
International Microbiology Aims and scope Submit manuscript

Abstract

Mitogen-activated protein kinases (MAPKs) are evolutionarily conserved signaling proteins involved in the regulation of most eukaryotic cellular processes. They are downstream components of essential signal transduction pathways activated by the external stimuli, in which the signal is conveyed through phosphorylation cascades. The excellent genetic and biochemical tractability of simple eukaryotes such as Saccharomyces cerevisiae has significantly contributed to gain fundamental information into the physiology of these key proteins. The budding yeast MAPK Slt2 was identified 30 years ago and was later revealed as a fundamental element of the cell wall integrity (CWI) pathway, one of the five MAPK routes of S. cerevisiae. As occurs with other MAPKs, whereas Slt2 displays the core typical structural traits of eukaryotic protein kinases, it also features conserved domains among MAPKs that allow an exquisite spatio-temporal regulation of their activity and binding to activating kinases, downregulatory phosphatases, or nuclear transcription factors. Additionally, Slt2 bears a regulatory extra C-terminal tail unique among S. cerevisiae MAPKs. Here, we review the structural and functional basis for the signaling role of Slt2 in the context of the molecular architecture of this important family of protein kinases.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Subscribe and save

Springer+ Basic
EUR 32.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or Ebook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1

Similar content being viewed by others

Data availability

Not applicable.

Code availability

Not applicable.

References

Download references

Acknowledgements

We wish to dedicate this article to the memory of Dr. Miguel Sánchez, who enthusiastically devoted his passion and effort to the identification and characterization of this protein kinase. We thank César Nombela for his support and colleagues from Unit 3 of the Departamento de Microbiología y Parasitología at UCM for their useful comments and discussions. We also thank Ministerio de Ciencia, Innovación y Universidades (Spain) (grant PID2019-105342GB-I00), and Comunidad de Madrid (Spain) and European Structural and Investment Funds (grant S2017/BMD-3691 InGEMICS-CM) for funding, and the Universidad Complutense de Madrid (Spain) and Ministerio de Ciencia, Innovación y Universidades (Spain), for supporting G.G.-R. and A.S.-M. predoctoral contracts.

Funding

Grant PID2019-105342 GB-I00 from the Ministerio de Ciencia, Innovación y Universidades (Spain), to M.M., and grant S2017/BMD-3691 (InGEMICS-CM) from the Comunidad de Madrid (Spain) and European Structural and Investment Funds to M.M.

Author information

Authors and Affiliations

Authors

Contributions

All authors, Gema González-Rubio, Ángela Sellers-Moya, Humberto Martín, and María Molina, contributed to conceiving and designing the study and writing of the paper.

Corresponding authors

Correspondence to Humberto Martín or María Molina.

Ethics declarations

Ethics approval and consent to participate

Not applicable.

Consent for publication

Not applicable.

Conflict of interest

The authors declare no competing interests.

Additional information

Publisher's note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

González-Rubio, G., Sellers-Moya, Á., Martín, H. et al. A walk-through MAPK structure and functionality with the 30-year-old yeast MAPK Slt2. Int Microbiol 24, 531–543 (2021). https://doi.org/10.1007/s10123-021-00183-z

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10123-021-00183-z

Keywords

Navigation