Differential response of photosynthetic apparatus towards alkaline pH treatment in NIES-39 and PCC 7345 strains of Arthrospira platensis

Abstract

Alkaline stress is one of the severe abiotic stresses, which is not well studied so far, especially among cyanobacteria. To affirm the characteristics of alkaline stress and the subsequent adaptive responses in Arthrospira platensis NIES-39 and Arthrospira platensis PCC 7345, photosynthetic pigments, spectral properties of thylakoids, PSII and PSI activities, and pigment-protein profiles of thylakoids under different pH regimes were examined. The accessory pigments showed a pH-mediated sensitivity. The pigment-protein complexes of thylakoids are also affected, resulting in the altered fluorescence emission profile. At pH 11, a possible shift of the PBsome antenna complex from PSII to PSI is observed. PSII reaction center is found to be more susceptible to alkaline stress in comparison to the PSI. In Arthrospira platensis NIES-39 at pH 11, a drop of 68% in the oxygen evolution with a significant increase of PSI activity by 114% is recorded within 24 h of pH treatment. Alterations in the cellular ultrastructure of Arthrospira platensis NIES-39 at pH 11 were observed, along with the increased number of plastoglobules attached with the thylakoid membranes. Arthrospira platensis NIES-39 is more adaptable to pH variation than Arthrospira platensis PCC 7345.

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Data availability

All the data generated and analyzed during this study are included in this published article along and the supplementary files.

Abbreviations

CBB G-250:

Coomassie Brilliant Blue G-250

Chl:

Chlorophyll

DCMU:

1,1-Dichloro methyl diphenyl urea

DCPIP:

2,5-Dichlorophenol indophenol

LiDS:

Lithium dodecyl sulfate

MV:

Methyl viologen

NIES:

National Institute of Environmental Studies

pBQ:

para-Benzoquinone

PBsomes:

Phycobilisomes

PC:

Phycocyanin

PCC:

Pasteur Culture Collection

PEC:

Phycoerythrocyanin

PSI:

Photosystem I

PSII:

Photosystem II

TEM:

Transmission electron microscopy

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Acknowledgments

Monika M. Jangir acknowledges the financial support from the Department of Science & Technology, Government of India in the form of DST-INSPIRE fellowship. The authors wish to thank BITS, Pilani, for providing infrastructural facilities.

Funding

Monika M. Jangir received financial support from the Department of Science & Technology, Government of India in the form of DST-INSPIRE fellowship from December 2013 to 2018.

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B. Vani conceived the original idea. B. Vani and Shibasish Chowdhury supervised the findings and experiments of this work. Monika M. Jangir performed the experimental works, literature surveys, and initial manuscript writing. All authors equally provided critical feedback and helped shape the research, analysis, and final manuscript.

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Correspondence to Shibasish Chowdhury or Vani Bhagavatula.

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Jangir, M.M., Chowdhury, S. & Bhagavatula, V. Differential response of photosynthetic apparatus towards alkaline pH treatment in NIES-39 and PCC 7345 strains of Arthrospira platensis. Int Microbiol (2021). https://doi.org/10.1007/s10123-021-00160-6

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Keywords

  • Arthrospira platensis
  • Thylakoid
  • PBsomes
  • Alkalinity
  • PSII
  • PSI
  • TEM