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Bacterial rhizosphere community profile at different growth stages of Umorok (Capsicum chinense) and its response to the root exudates

  • Phazna Devi T. A.
  • Dinabandhu Sahoo
  • Aravind Setti
  • Chandradev Sharma
  • M. C. Kalita
  • Indira Devi S.Email author
Original Article

Abstract

The role of microflora is an indispensable part of the living organisms. Plants actively recruit specific microbial community to establish favorable habitat with the distinct microbiome, essentially unique for each species, offering new opportunities for plant growth and productivity. Umorok, an indigenous chili variety of northeastern India, production is highly affected by various factors; therefore, rhizosphere bacteria and their relationship with the root exudates released were analyzed to demonstrate rhizosphere bacterial impact on plant growth and health. Culturable and metagenomic bacterial DNA was characterized and the chemical nature of the root exudate was analyzed using chemotaxis assay after its basic analysis in HPLC. Juvenile stage exhibited diverse bacterial species of gammaproteobacteria, alphaproteobacteria, and actinobacteria but lacked the betaproteobacteria while the microbial diversity was reduced in flowering and fruiting stages. However, every growth stage maintained a similar amount of bacterial population regardless of diversity. The population of Pseudomonas, Bacillus, and Burkholderia species was increased several folds in flowering and fruiting stage. Further, the chemotaxis assay unveiled the advantage of root exudate chemical composition for specific microbial recruitment. The chemical composition analysis of root exudates showed substantial variation in the concentration of organic acids, phenolics, and flavonoids that are favoring unique bacterial species. Thus, root exudates confer and limit the related microbial population besides typical plant-bacterial synergetic association. This study emphasized information about the type of microbial load present in each growth stage, which is essential to develop a microbial consortia package for Umorok overall crop improvement.

Keywords

Rhizosphere Microflora Umorok Root exudate Chemotaxis 

Notes

Funding information

This study received financial support from the funding agency Department of Biotechnology (DBT), Ministry of Science & Technology, Government of India.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer Nature Switzerland AG 2019

Authors and Affiliations

  1. 1.Microbial Resources DivisionInstitute of Bioresources and Sustainable Development (IBSD), Takyelpat Institutional AreaImphalIndia
  2. 2.Department of GeneticsOsmania UniversityHyderabadIndia
  3. 3.Department of BiotechnologyGauhati UniversityGuwahatiIndia

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