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International Microbiology

, Volume 6, Issue 4, pp 259–261 | Cite as

Stress proteins of Clostridium perfringens type A immunoreact with antiserum from rabbits infected with gas gangrene

  • Norma Heredia
  • Elva Aréchiga
  • Ronald Labbé
  • Santos García
Research Article

Abstract

Various stressors were used to induce stress proteins in Clostridium perfringens. Cultures of C. perfringens FD-1041 were subjected to cold shock (28°C for 1 h), acid shock (pH 4.5 for 30 min), or heat shock (50°C for 30 min). Cells were lysed and protein samples were analyzed by immunoblotting with antiserum derived from rabbits suffering from gas gangrene. Eight cold shock proteins (approximate M r 101, 82, 70, 37, 22, 12, 10 and 6 kDa) and also eight heat shock proteins (approximate M r 101, 82, 70, 27, 22, 16, 12 and 10 kDa) were immunoreactive with the serum. No immunoreactive proteins were detected in samples subjected to acid shock proteins and purified DnaK protein was also non-immunoreactive with the serum. These immunogenic stress proteins may be important in regulating diseases caused by C. perfringens. Such proteins could be involved in cell survival mechanisms, serve as targets during infection, or play a role in recognition of the bacteria by the host.

Keywords

Clostridium perfringens Immunogenic proteins Stress proteins Stress response 

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Copyright information

© Springer-Verlag and SEM 2003

Authors and Affiliations

  • Norma Heredia
    • 1
  • Elva Aréchiga
    • 1
  • Ronald Labbé
    • 2
  • Santos García
    • 1
  1. 1.Facultad de Ciencias BiológicasUniversidad Autónoma de Nuevo LeónSan Nicolás N.L.Mexico
  2. 2.Food Science DepartmentUniversity of MassachusettsAmherstUSA

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