Gastric Cancer

, Volume 22, Issue 3, pp 463–472 | Cite as

Near infrared photoimmunotherapy using a fiber optic diffuser for treating peritoneal gastric cancer dissemination

  • Tadanobu Nagaya
  • Shuhei Okuyama
  • Fusa Ogata
  • Yasuhiro Maruoka
  • Peter L. Choyke
  • Hisataka KobayashiEmail author
Original Article



Peritoneal dissemination (PD) of abdominal malignancies is a common form of metastasis and its presence signals a poor prognosis. New treatment is required for patients with PD. Near infrared photoimmunotherapy (NIR-PIT) is a highly selective tumor treatment that employs an antibody–photo-absorber conjugate (APC). In this study, we investigate in vitro and in vivo efficacy of trastuzumab (tra)-IR700DX NIR-PIT on a human epidermal growth factor receptor type 2 (HER2)-positive gastric cancer cell line.


NIR-PIT effects were investigated in vitro and in vivo. Disseminated peritoneal implants mice were separated into 5 groups: (1) no treatment; (2) tra-IR700 i.v. only; (3) NIR light only; (4) NIR-PIT; (5) repeated NIR-PIT. The peritoneal cavity was irradiated with NIR light using a fiber optic diffuser delivered through the catheter.


Specific binding and cell-specific killing was observed after NIR-PIT in vitro. In the in vivo study, fluorescence endoscopy showed high tumor accumulation of tra-IR700 within tumors. Significantly prolonged survival was achieved in the three treatment groups (tra-IR700 i.v. only, NIR-PIT, and repeated NIR-PIT groups) compared with control and NIR light only group (p < 0.05 for tra-IR700 i.v. only, p < 0.01 for NIR-PIT, and p < 0.0001 for repeated NIR-PIT). Moreover, most prolonged survival was shown for the repeated NIR-PIT group (p < 0.0001 vs tra-IR700 i.v. only, p < 0.01 vs NIR-PIT).


NIR-PIT using a fiber optic diffuser to deliver light is a promising candidate for the treatment of disseminated peritoneal metastases and could be readily translated to humans.


Near infrared photoimmunotherapy Gastric cancer Fiber optic light probes Monoclonal antibodies Molecular imaging 



All authors were supported by the Intramural Research Program of the NIH, NCI, Center for Cancer Research.

Compliance with ethical standards

Conflict of interest

No potential conflicts of interest were disclosed.

Ethical standards

All institutional and national guidelines for the care and use of laboratory animals were followed.

Supplementary material

Supplementary material 1 (AVI 1744 KB)

10120_2018_871_MOESM2_ESM.avi (5.4 mb)
Supplementary material 2 (AVI 5579 KB)


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Copyright information

© This is a U.S. government work and its text is not subject to copyright protection in the United States; however, its text may be subject to foreign copyright protection 2018

Authors and Affiliations

  1. 1.Molecular Imaging Program, Center for Cancer Research, National Cancer InstituteNational Institutes of HealthBethesdaUSA

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