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Gastric Cancer

, Volume 11, Issue 1, pp 37–46 | Cite as

Minichromosome maintenance 2 (MCM2) immunoreactivity in stage III human gastric carcinoma: clinicopathological significance

  • Naruo Tokuyasu
  • Kohei Shomori
  • Keisuke Nishihara
  • Hiroki Kawaguchi
  • Shinji Fujioka
  • Kensaku Yamaga
  • Masahide Ikeguchi
  • Hisao Ito
Original Article

Abstract

Background

The origin licensing factor minichromosome maintenance 2 (MCM2) has recently been identified as a critical regulator of proliferation in both normal and neoplastic cells. This study examined whether MCM2 expression was of prognostic relevance in patients with stage III gastric carcinoma and whether the expression of this marker showed any correlation with clinicopathological characteristics. In addition, we evaluated whether the expression of this proliferation marker was correlated with that of another marker, Ki-67, in gastric carcinoma.

Methods

We examined the immunohistochemical expression of MCM2, Ki-67, and p53 in 103 surgically removed stage III gastric carcinomas, which consisted of 60 intestinal-type and 43 diffuse-type carcinomas. The labeling indices (LIs) of MCM2 and Ki-67 in cancer cells were compared with clinicopathological characteristics, p53 expression, and overall survival rates.

Results

The mean MCM2 and Ki-67 LIs were 69.1 ± 11.8% and 48.2 ± 14.5%, respectively, in the intestinal carcinomas, and 43.7 ± 9.9% and 24.9 ± 11.0%, respectively, in the diffuse carcinomas. The LIs of these proteins revealed no significant association with clinicopathological characteristics or with p53 expression in the carcinomas. Kaplan-Meier survival curves showed that, in the patients with diffuse carcinoma, those with higher MCM2 LIs had a poorer prognosis (P < 0.05), but the MCM2 LI was not correlated with prognosis for those with intestinal carcinoma (P = 0.25). Ki-67 expression had no significant correlation with prognosis in either intestinal-type or diffuse-type carcinomas. Multivariate Cox regression analysis confirmed that MCM2 was an independent prognostic factor in patients with diffuse carcinoma.

Conclusion

Our data suggest that MCM2 is a useful prognostic marker in patients stage III diffuse-type gastric carcinoma.

Key words

Minichromosome maintenance protein 2 (MCM2) Gastric carcinoma Ki-67 p53 Prognostic value 

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Copyright information

© The International Gastric Cancer Association and The Japanese Gastric Cancer Association 2008

Authors and Affiliations

  • Naruo Tokuyasu
    • 1
  • Kohei Shomori
    • 1
  • Keisuke Nishihara
    • 1
  • Hiroki Kawaguchi
    • 3
  • Shinji Fujioka
    • 1
  • Kensaku Yamaga
    • 1
  • Masahide Ikeguchi
    • 2
  • Hisao Ito
    • 1
  1. 1.Division of Organ Pathology, Department of Microbiology and Pathology, Faculty of MedicineTottori UniversityTottoriJapan
  2. 2.Division of Surgical Oncology, Department of Surgery, Faculty of MedicineTottori UniversityTottoriJapan
  3. 3.Division of SurgeryYonago Medical Center HospitalTottoriJapan

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