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Genetic detection of free cancer cells in the peritoneal cavity of the patient with gastric cancer: present status and future perspectives

Gastric Cancer volume 10, pages 197–204 (2007)Cite this article

Abstract

The purpose of this review is to examine the current status and future perspectives of the molecular analysis of peritoneal lavage fluid in patients with gastric cancer. During the past 10 years, the polymerase chain reaction (PCR) has been applied for the molecular detection of free cancer cells in the abdominal cavity of patients with gastric cancer, and its clinical significance in establishing the presence of peritoneal dissemination has been assessed by several groups especially in Japan. The majority of these studies have confirmed the predictive value of the molecular detection of peritoneal metastasis and recurrence using peritoneal lavage fluid. Based on these findings, since April 2006, the genetic diagnosis of body fluids has been included in the Japanese Government public health insurance program for patients with solid tumors. However, there are still many obstacles to overcome before the genetic diagnosis of micrometastasis can be considered a routine laboratory assay. Here we review the importance of the molecular detection of cancer cells in the abdominal cavity, and the molecular techniques used for such diagnosis; we also provide some clinical examples to illustrate the value of molecular diagnosis.

References

  1. JB Dupont JR Lee GR Burton I Cohn SuffixJr (1978) ArticleTitleAdenocarcinoma of the stomach: review of 1497 cases Cancer 41 941–7 Occurrence Handle638980 Occurrence Handle10.1002/1097-0142(197803)41:3<941::AID-CNCR2820410323>3.0.CO;2-M

    PubMed  Article  Google Scholar 

  2. E Yamada S Miyaishi H Nakazato K Kato T Kito H Takagi et al. (1980) ArticleTitleThe surgical treatment of cancer of the stomach Int Surg 65 387–99 Occurrence Handle6161095 Occurrence Handle1:STN:280:Bi6D1Mzhslw%3D

    PubMed  CAS  Google Scholar 

  3. E Bando Y Yonemura Y Takeshita K Taniguchi T Yasui Y Yoshimitsu et al. (1999) ArticleTitleIntraoperative lavage for cytological examination in 1297 patients with gastric carcinoma Am J Surg 178 256–62 Occurrence Handle10527450 Occurrence Handle10.1016/S0002-9610(99)00162-2 Occurrence Handle1:STN:280:DyaK1MvlsVGktg%3D%3D

    PubMed  Article  CAS  Google Scholar 

  4. JJ Bonenkamp I Songun J Hermans CJ van de Velde (1996) ArticleTitlePrognostic value of positive cytology findings from abdominal washings in patients with gastric cancer Br J Surg 83 672–4 Occurrence Handle8689216 Occurrence Handle10.1002/bjs.1800830526 Occurrence Handle1:STN:280:BymB1MnisFU%3D

    PubMed  Article  CAS  Google Scholar 

  5. S Koga N Kaibara Y Iitsuka H Kudo A Kimura H Hiraoka (1984) ArticleTitlePrognostic significance of intra-peritoneal free cancer cells in gastric cancer patients J Cancer Res Clin Oncol 108 236–8 Occurrence Handle6470030 Occurrence Handle10.1007/BF00402474 Occurrence Handle1:STN:280:BiuB1cbmsFU%3D

    PubMed  Article  CAS  Google Scholar 

  6. InstitutionalAuthorNameJapanese Gastric Cancer Association (1998) ArticleTitleJapanese classification of gastric carcinoma – 2nd English edition Gastric Cancer 1 10–24 Occurrence Handle11957040 Occurrence Handle10.1007/PL00011681

    PubMed  Article  Google Scholar 

  7. S Noura H Yamamoto T Ohnishi N Masuda T Matsumoto O Takayama et al. (2002) ArticleTitleComparative detection of lymph node micrometastases of stage II colorectal cancer by reverse transcriptase polymerase chain reaction and immunohistochemistry J Clin Oncol 20 4232–41 Occurrence Handle12377967 Occurrence Handle10.1200/JCO.2002.10.023 Occurrence Handle1:CAS:528:DC%2BD38XnsFyqsb0%3D

    PubMed  Article  CAS  Google Scholar 

  8. S Yoshioka Y Fujiwara Y Sugita Y Okada M Yano S Tamura et al. (2002) ArticleTitleReal-time rapid reverse transcriptase-polymerase chain reaction for intraoperative diagnosis of lymph node micrometastasis: clinical application for cervical lymph node dissection in esophageal cancers Surgery 132 34–40 Occurrence Handle12110793 Occurrence Handle10.1067/msy.2002.125306

    PubMed  Article  Google Scholar 

  9. Y Okada Y Fujiwara H Yamamoto Y Sugita T Yasuda Y Doki et al. (2001) ArticleTitleGenetic detection of lymph node micrometastases in patients with gastric carcinoma by multiple-marker reverse transcriptase-polymerase chain reaction assay Cancer 92 2056–64 Occurrence Handle11596020 Occurrence Handle10.1002/1097-0142(20011015)92:8<2056::AID-CNCR1545>3.0.CO;2-L Occurrence Handle1:CAS:528:DC%2BD3MXot12rt7c%3D

    PubMed  Article  CAS  Google Scholar 

  10. Y Sugita Y Fujiwara H Taniguchi T Mori T Ishii H Niwa et al. (2003) ArticleTitleQuantitative molecular diagnosis of peritoneal lavage fluid for prediction of peritoneal recurrence in gastric cancer Int J Oncol 23 1419–23 Occurrence Handle14532985 Occurrence Handle1:CAS:528:DC%2BD3sXptFCrs7g%3D

    PubMed  CAS  Google Scholar 

  11. Y Kodera H Nakanishi S Ito Y Yamamura M Fujiwara M Koike et al. (2005) ArticleTitlePrognostic significance of intraperitoneal cancer cells in gastric carcinoma: detection of cytokeratin 20 mRNA in peritoneal washes, in addition to detection of carcinoembryonic antigen Gastric Cancer 8 142–8 Occurrence Handle16086116 Occurrence Handle10.1007/s10120-005-0318-7 Occurrence Handle1:CAS:528:DC%2BD2MXntVeht7o%3D

    PubMed  Article  CAS  Google Scholar 

  12. H Nakanishi Y Kodera A Torii T Hirai Y Yamamura T Kato et al. (1997) ArticleTitleDetection of carcinoembryonic antigen-expressing free tumor cells in peritoneal washes from patients with gastric carcinoma by polymerase chain reaction Jpn J Cancer Res 88 687–92 Occurrence Handle9310142 Occurrence Handle1:CAS:528:DyaK2sXltlSrtLc%3D

    PubMed  CAS  Google Scholar 

  13. H Nakanishi Y Kodera Y Yamamura S Ito T Kato T Ezaki et al. (2000) ArticleTitleRapid quantitative detection of carcinoembryonic antigen-expressing free tumor cells in the peritoneal cavity of gastric-cancer patients with real-time RT-PCR on the LightCycler Int J Cancer 89 411–7 Occurrence Handle11008202 Occurrence Handle10.1002/1097-0215(20000920)89:5<411::AID-IJC3>3.0.CO;2-5 Occurrence Handle1:CAS:528:DC%2BD3cXnsFWmsrg%3D

    PubMed  Article  CAS  Google Scholar 

  14. Y Yonemura T Fujimura I Ninomiya BS Kim E Bandou T Sawa et al. (2001) ArticleTitlePrediction of peritoneal micrometastasis by peritoneal lavaged cytology and reverse transcriptase-polymerase chain reaction for matrix metalloproteinase-7 mRNA Clin Cancer Res 7 1647–53 Occurrence Handle11410502 Occurrence Handle1:CAS:528:DC%2BD3MXltV2hsrY%3D

    PubMed  CAS  Google Scholar 

  15. P Schmidt M Thiele C Rudroff A Vaz M Schilli K Friedrich et al. (2001) ArticleTitleDetection of tumor cells in peritoneal lavages from patients with gastrointestinal cancer by multiplex reverse transcriptase PCR Hepatogastroenterology 48 1675–9 Occurrence Handle11813599 Occurrence Handle1:CAS:528:DC%2BD38XislChsA%3D%3D

    PubMed  CAS  Google Scholar 

  16. Y Kodera H Nakanishi Y Yamamura Y Shimizu A Torii T Hirai et al. (1998) ArticleTitlePrognostic value and clinical implications of disseminated cancer cells in the peritoneal cavity detected by reverse transcriptase-polymerase chain reaction and cytology Int J Cancer 79 429–33 Occurrence Handle9699538 Occurrence Handle10.1002/(SICI)1097-0215(19980821)79:4<429::AID-IJC20>3.0.CO;2-Z Occurrence Handle1:CAS:528:DyaK1cXltVens74%3D

    PubMed  Article  CAS  Google Scholar 

  17. Y Kodera H Nakanishi Y Yamamura Y Kanemitsu Y Shimizu T Hirai et al. (2002) ArticleTitleQuantitative detection of disseminated free cancer in peritoneal washes with real-time reverse transcriptase polymerase chain reaction Ann Surg 235 499–506 Occurrence Handle11923605 Occurrence Handle10.1097/00000658-200204000-00007

    PubMed  Article  Google Scholar 

  18. J Cai M Ikeguchi S Tsujitani M Maeta J Liu N Kaibara (2001) ArticleTitleSignificant correlation between micrometastasis in the lymph nodes and reduced expression of E-cadherin in early gastric cancer Gastric Cancer 4 66–74 Occurrence Handle11706763 Occurrence Handle10.1007/PL00011726 Occurrence Handle1:STN:280:DC%2BD3MnltlKqsQ%3D%3D

    PubMed  Article  CAS  Google Scholar 

  19. E Lee Y Chae I Kim J Choi B Yeom AS Leong (2002) ArticleTitlePrognostic relevance of immunohistochemically detected lymph node micrometastasis in patients with gastric carcinoma Cancer 94 2867–73 Occurrence Handle12115374 Occurrence Handle10.1002/cncr.10562

    PubMed  Article  Google Scholar 

  20. T Fukagawa M Sasako GB Mann T Sano H Katai K Maruyama et al. (2001) ArticleTitleImmunohistochemically detected micrometastases of the lymph nodes in patients with gastric carcinoma Cancer 15 92 753–60 Occurrence Handle10.1002/1097-0142(20010815)92:4<753::AID-CNCR1379>3.0.CO;2-5 Occurrence Handle1:STN:280:DC%2BD3MvpvFahsA%3D%3D

    Article  CAS  Google Scholar 

  21. P Morgagni L Saragoni E Scarpi PS Zattini A Zaccaroni D Morgagni et al. (2003) ArticleTitleLymph node micrometastases in early gastric cancer and their impact on prognosis World J Surg 27 558–61 Occurrence Handle12715223 Occurrence Handle10.1007/s00268-003-6797-y

    PubMed  Article  Google Scholar 

  22. Y Maehara M Yamamoto S Oda H Baba T Kusumoto S Ohno et al. (1996) ArticleTitleCytokeratin-positive cells in bone marrow for identifying distant micrometastasis of gastric cancer Br J Cancer 73 83–7 Occurrence Handle8554989 Occurrence Handle1:STN:280:BymC2cnmtVI%3D

    PubMed  CAS  Google Scholar 

  23. R Macadam A Sarela J Wilson K MacLennan P Guillou (2003) ArticleTitleBone marrow micrometastases predict early post-operative recurrence following surgical resection of oesophageal and gastric carcinoma Eur J Surg Oncol 29 450–4 Occurrence Handle12798750 Occurrence Handle10.1016/S0748-7983(03)00029-5

    PubMed  Article  Google Scholar 

  24. XM Chen GY Chen ZR Wang FS Zhu XL Wang X Zhang (2004) ArticleTitleDetection of micrometastasis of gastric carcinoma in peripheral blood circulation World J Gastroenterol 10 804–8 Occurrence Handle15040021 Occurrence Handle1:CAS:528:DC%2BD2cXjtl2htbw%3D

    PubMed  CAS  Google Scholar 

  25. N Mori M Oka S Hazama N Iizuka K Yamamoto S Yoshino et al. (2000) ArticleTitleDetection of telomerase activity in peritoneal lavage fluid from patients with gastric cancer using immunomagnetic beads Br J Cancer 83 1026–32 Occurrence Handle10993650 Occurrence Handle10.1054/bjoc.2000.1408 Occurrence Handle1:CAS:528:DC%2BD3cXnsF2hs7g%3D

    PubMed  Article  CAS  Google Scholar 

  26. C Sakakura M Takemura A Hagiwara K Shimomura K Miyagawa S Nakashima et al. (2004) ArticleTitleOverexpression of dopa decarboxylase in peritoneal dissemination of gastric cancer and its potential as a novel marker for the detection of peritoneal micrometastases with real-time RT-PCR Br J Cancer 90 665–71 Occurrence Handle14760382 Occurrence Handle10.1038/sj.bjc.6601544 Occurrence Handle1:CAS:528:DC%2BD2cXoslKjsw%3D%3D

    PubMed  Article  CAS  Google Scholar 

  27. K Shimomura C Sakakura M Takemura T Takagi K Fukuda S Kin et al. (2004) ArticleTitleCombination of L-3-phosphoserine phosphatase and CEA using real-time RT-PCR improves accuracy in detection of peritoneal micrometastasis of gastric cancer Anticancer Res 24 1113–20 Occurrence Handle15154633 Occurrence Handle1:CAS:528:DC%2BD2cXkslynsLo%3D

    PubMed  CAS  Google Scholar 

  28. K Mori T Suzuki H Uozaki H Nakanishi T Ueda Y Matsuno et al. (2007) ArticleTitleDetection of minimal gastric cancer cells in peritoneal washings by focused microarray analysis with multiple markers: clinical implications Ann Surg Oncol 14 1694–702 Occurrence Handle17294072 Occurrence Handle10.1245/s10434-006-9321-4

    PubMed  Article  Google Scholar 

  29. M Benevolo (1998) ArticleTitleDiagnostic and prognostic value of peritoneal immunocytology in gastric cancer J Clin Oncol 16 3406–11 Occurrence Handle9779720 Occurrence Handle1:STN:280:DyaK1cvltFSjsg%3D%3D

    PubMed  CAS  Google Scholar 

  30. P Vogel J Ruschoff S Kummel H Zirngibl F Hofstadter W Hohenberger et al. (1999) ArticleTitleImmunocytology improves prognostic impact of peritoneal tumour cell detection compared to conventional cytology in gastric cancer Eur J Surg Oncol 25 515–9 Occurrence Handle10527600 Occurrence Handle10.1053/ejso.1999.0688 Occurrence Handle1:STN:280:DyaK1MvlvFejtw%3D%3D

    PubMed  Article  CAS  Google Scholar 

  31. H Nekarda C Gess M Stark JD Mueller U Fink U Schenck et al. (1999) ArticleTitleImmunocytochemically detected free peritoneal tumour cells (FPTC) are a strong prognostic factor in gastric carcinoma Br J Cancer 79 611–9 Occurrence Handle10027338 Occurrence Handle10.1038/sj.bjc.6690096 Occurrence Handle1:STN:280:DyaK1M7kvFGrtg%3D%3D

    PubMed  Article  CAS  Google Scholar 

  32. N Abe T Watanabe H Toda H Machida K Suzuki T Masaki et al. (2001) ArticleTitlePrognostic significance of carcinoembryonic antigen levels in peritoneal washes in patients with gastric cancer Am J Surg 181 356–61 Occurrence Handle11438272 Occurrence Handle10.1016/S0002-9610(01)00585-2 Occurrence Handle1:STN:280:DC%2BD3MzotV2jtg%3D%3D

    PubMed  Article  CAS  Google Scholar 

  33. M Nishiyama I Takashima T Tanaka K Yoshida T Toge N Nagata et al. (1995) ArticleTitleCarcinoembryonic antigen levels in the peritoneal cavity: useful guide to peritoneal recurrence and prognosis for gastric cancer World J Surg 19 133–7 Occurrence Handle7740800 Occurrence Handle10.1007/BF00316997 Occurrence Handle1:STN:280:ByqB2MbjsFc%3D

    PubMed  Article  CAS  Google Scholar 

  34. M Yamamoto H Baba Y Kakeji K Endo Y Ikeda Y Toh et al. (2004) ArticleTitlePrognostic significance of tumor markers in peritoneal lavage in advanced gastric cancer Oncology 67 19–26 Occurrence Handle15459491 Occurrence Handle10.1159/000080281 Occurrence Handle1:CAS:528:DC%2BD2cXotVGgs7c%3D

    PubMed  Article  CAS  Google Scholar 

  35. JY Wang SR Lin CY Lu CC Chen DC Wu CY Chai et al. (2005) ArticleTitleGastric cancer cell detection in peritoneal lavage: RT-PCR for carcinoembryonic antigen transcripts versus the combined cytology with peritoneal carcinoembryonic antigen levels Cancer Lett 223 129–35 Occurrence Handle15890245 Occurrence Handle10.1016/j.canlet.2004.09.031 Occurrence Handle1:CAS:528:DC%2BD2MXktVOmsrY%3D

    PubMed  Article  CAS  Google Scholar 

  36. D Horibe T Ochiai H Shimada T Tomonaga F Nomura M Gun et al. (2007) ArticleTitleRapid detection of metastasis of gastric cancer using reverse transcription loop-mediated isothermal amplification Int J Cancer 120 1063–9 Occurrence Handle17139607 Occurrence Handle10.1002/ijc.22397 Occurrence Handle1:CAS:528:DC%2BD2sXhsVWhs78%3D

    PubMed  Article  CAS  Google Scholar 

  37. T Ishii Y Fujiwara S Ohnaka T Hayashi H Taniguchi S Takiguchi et al. (2004) ArticleTitleRapid genetic diagnosis with the transcription-reverse transcription concerted reaction system for cancer micrometastasis Ann Surg Oncol 11 778–85 Occurrence Handle15289240 Occurrence Handle10.1245/ASO.2004.12.043

    PubMed  Article  Google Scholar 

  38. Y Ko M Klinz G Totzke I Gouni-Berthold A Sachinidis H Vetter (1998) ArticleTitleLimitations of the reverse transcription-polymerase chain reaction method for the detection of carcinoembryonic antigen-positive tumor cells in peripheral blood Clin Cancer Res 4 2141–6 Occurrence Handle9748132 Occurrence Handle1:STN:280:DyaK1cvhvV2hug%3D%3D

    PubMed  CAS  Google Scholar 

  39. Y Ko E Grunewald G Totzke M Klinz S Fronhoffs I Gouni-Berthold et al. (2000) ArticleTitleHigh percentage of false-positive results of cytokeratin 19 RT-PCR in blood: a model for the analysis of illegitimate gene expression Oncology 59 81–8 Occurrence Handle10895072 Occurrence Handle10.1159/000012126 Occurrence Handle1:CAS:528:DC%2BD3cXls1Gms7s%3D

    PubMed  Article  CAS  Google Scholar 

  40. JC Goeminne T Guillaume M Salmon JP Machiels V D'Hondt M Symann (1999) ArticleTitleUnreliability of carcinoembryonic antigen (CEA) reverse transcriptase-polymerase chain reaction (RT-PCR) in detecting contaminating breast cancer cells in peripheral blood stem cells due to induction of CEA by growth factors Bone Marrow Transplant 24 769–75 Occurrence Handle10516681 Occurrence Handle10.1038/sj.bmt.1701966 Occurrence Handle1:STN:280:DyaK1MvkslGgsQ%3D%3D

    PubMed  Article  CAS  Google Scholar 

  41. T Mori Y Fujiwara Y Sugita T Azama T Ishii K Taniguchi et al. (2004) ArticleTitleApplication of molecular diagnosis for detection of peritoneal micrometastasis and evaluation of preoperative chemotherapy in advanced gastric carcinoma Ann Surg Oncol 11 14–20 Occurrence Handle14699028 Occurrence Handle10.1007/BF02524340

    PubMed  Article  Google Scholar 

  42. K Yamamoto S Shimada M Hirota Y Yagi M Matsuda H Baba (2005) ArticleTitleEIPL (extensive intraoperative peritoneal lavage) therapy significantly reduces peritoneal recurrence after pancreatectomy in patients with pancreatic cancer Int J Oncol 27 1321–8 Occurrence Handle16211228

    PubMed  Google Scholar 

  43. S Fujimoto RD Shrestha M Kokubun M Ohta M Takahashi K Kobayashi et al. (1988) ArticleTitleIntraperitoneal hyperthermic perfusion combined with surgery effective for gastric cancer patients with peritoneal seeding Ann Surg 208 36–41 Occurrence Handle3133994 Occurrence Handle10.1097/00000658-198807000-00005 Occurrence Handle1:STN:280:BieB28fivFA%3D

    PubMed  Article  CAS  Google Scholar 

  44. R Hamazoe M Maeta N Kaibara (1994) ArticleTitleIntraperitoneal thermo-chemotherapy for prevention of peritoneal recurrence of gastric cancer. Final results of randomized controlled study Cancer 73 2048–52 Occurrence Handle8156509 Occurrence Handle10.1002/1097-0142(19940415)73:8<2048::AID-CNCR2820730806>3.0.CO;2-Q Occurrence Handle1:STN:280:ByuB3cjps1U%3D

    PubMed  Article  CAS  Google Scholar 

  45. DK Armstrong B Bundy L Wenzel HQ Huang R Baergen S Lele et al. (2006) ArticleTitleIntraperitoneal cisplatin and paclitaxel in ovarian cancer N Engl J Med 354 34–43 Occurrence Handle16394300 Occurrence Handle10.1056/NEJMoa052985 Occurrence Handle1:CAS:528:DC%2BD28XhvV2qsQ%3D%3D

    PubMed  Article  CAS  Google Scholar 

  46. S Sakuramoto M Sasako T Yamaguchi T Kinoshita M Fujii A Nashimoto et al. (2007) ArticleTitleAdjuvant chemotherapy for gastric cancer with S-1, an oral fluoropyrimidine N Engl J Med 357 1810–20 Occurrence Handle17978289 Occurrence Handle10.1056/NEJMoa072252 Occurrence Handle1:CAS:528:DC%2BD2sXht1Kit7bI

    PubMed  Article  CAS  Google Scholar 

  47. T Fujimura T Ohta H Kitagawa S Fushida GI Nishimura Y Yonemura et al. (1998) ArticleTitleTrypsinogen expression and early detection for peritoneal dissemination in gastric cancer J Surg Oncol 69 71–5 Occurrence Handle9808508 Occurrence Handle10.1002/(SICI)1096-9098(199810)69:2<71::AID-JSO5>3.0.CO;2-R Occurrence Handle1:STN:280:DyaK1M%2FitlKqtg%3D%3D

    PubMed  Article  CAS  Google Scholar 

  48. Y Yonemura Y Endou T Fujimura S Fushida E Bandou K Kinoshita et al. (2001) ArticleTitleDiagnostic value of preoperative RT-PCR-based screening method to detect carcinoembryonic antigen-expressing free cancer cells in the peritoneal cavity from patients with gastric cancer ANZ J Surg 71 521–8 Occurrence Handle11527261 Occurrence Handle10.1046/j.1440-1622.2001.02187.x Occurrence Handle1:STN:280:DC%2BD3MvosFWgtQ%3D%3D

    PubMed  Article  CAS  Google Scholar 

  49. K Oyama M Terashima A Takagane C Maesawa (2004) ArticleTitlePrognostic significance of peritoneal minimal residual disease in gastric cancer detected by reverse transcription-polymerase chain reaction Br J Surg 91 435–43 Occurrence Handle15048743 Occurrence Handle10.1002/bjs.4455 Occurrence Handle1:STN:280:DC%2BD2c7lsVWgtg%3D%3D

    PubMed  Article  CAS  Google Scholar 

  50. S Ito H Nakanishi Y Kodera Y Mochizuki M Tatematsu Y Yamamura (2005) ArticleTitleProspective validation of quantitative CEA mRNA detection in peritoneal washes in gastric carcinoma patients Br J Cancer 93 986–92 Occurrence Handle16205696 Occurrence Handle10.1038/sj.bjc.6602802 Occurrence Handle1:CAS:528:DC%2BD2MXhtFChsL3O

    PubMed  Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Gastroenterological Surgery, Graduate School of Medicine, Osaka University, 2-2 Yamadaoka (E-2), Suita, 565-0871, Japan

    Yoshiyuki Fujiwara, Yuichiro Doki, Hirokazu Taniguchi, Itsuro Sohma, Shuji Takiguchi, Hiroshi Miyata, Makoto Yamasaki & Morito Monden

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  1. Yoshiyuki Fujiwara
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  2. Yuichiro Doki
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  3. Hirokazu Taniguchi
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Fujiwara, Y., Doki, Y., Taniguchi, H. et al. Genetic detection of free cancer cells in the peritoneal cavity of the patient with gastric cancer: present status and future perspectives. Gastric Cancer 10, 197–204 (2007). https://doi.org/10.1007/s10120-007-0436-5

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Key words

  • Gastric cancer
  • Peritoneal dissemination
  • Micrometastasis
  • Molecular biology
  • RT-PCR
  • TRC