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Modulation of heat shock protein expression and cytokine levels in MCF-7 cells through photodynamic therapy

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Abstract

In this study, we assess the impact of photodynamic therapy (PDT) using aluminum phthalocyanine tetrasulfonate (AlPcS4) on the viability and cellular stress responses of MCF-7 breast cancer cells. Specifically, we investigate changes in cell viability, cytokine production, and the expression of stress-related genes. Experimental groups included control cells, those treated with AlPcS4 only, light-emitting diode (LED) only, and combined PDT. To evaluate these effects on cell viability, cytokine production, and the expression of stress-related genes, techniques such as 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) assay, enzyme-linked immunosorbent assays (ELISA), and real-time quantitative PCR (RT‒qPCR) were employed. Our findings reveal how PDT with AlPcS4 modulates mitochondrial activity and cytokine responses, shedding light on the cellular pathways essential for cell survival and stress adaptation. This work enhances our understanding of PDT's therapeutic potential and mechanisms in treating breast cancer.

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Funding

Fundação de Amparo a Pesquisa do Estado de São Paulo (FAPESP), grant number 2016/17984–1; 2021/09418–4; FINEP grant number 01.18.0053.00.

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Authors and Affiliations

  1. Universidade Do Vale Do Paraíba, Av Shishima Hifumi 2911, Urbanova, São José dos Campos, SP, 12244-000, Brazil

    Mariela Inês Batista dos Santos, Bruno Henrique Godoi & Cristina Pacheco-Soares

  2. Universidade Estadual Paulista Júlio de Mesquita Filho, Av. Eng. Francisco José Longo, 777 Jardim São Dimas, São José dos Campos, SP, 12245-000, Brazil

    Newton Soares Da Silva, Luciane Dias Oliveira & Lucas de Paula Ramos

  3. São José Dos Campos, Brazil

    Newton Soares Da Silva, Luciane Dias Oliveira & Lucas de Paula Ramos

  4. Universidade de São Paulo- R. da Reitoria, 374 Cidade Universitária, Butantã, São Paulo, SP, 05508-220, Brazil

    Ricardo Cesar Cintra

  5. University Claude Bernard Lyon, Laboratory Health Systemic Process - P2S, UR4129, Faculty of Medicine Laennec, rue Guillaume Paradin, 69008, Lyon, France

    Lucas de Paula Ramos

Authors
  1. Mariela Inês Batista dos Santos
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  2. Bruno Henrique Godoi
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  4. Luciane Dias Oliveira
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  7. Cristina Pacheco-Soares
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Correspondence to Cristina Pacheco-Soares.

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dos Santos, M.I.B., Godoi, B.H., Da Silva, N.S. et al. Modulation of heat shock protein expression and cytokine levels in MCF-7 cells through photodynamic therapy. Lasers Med Sci 39, 135 (2024). https://doi.org/10.1007/s10103-024-04092-1

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