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The effect of different treatment protocols with diode laser on regeneration in axonetmesis ınjuries of the ınferior alveolar nerve: an animal study

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The inferior alveolar nerve can be damaged during dental procedures, leading to symptoms, such as tingling, numbness, and reduced quality of life. Recovery depends on factors such as medications, surgery, and photobiomodulation therapy. Photobiomodulation therapy has shown the potential to improve nerve function and reduce regeneration time; however, there is no standard treatment protocol yet. This study aimed to examine the effect of diode lasers on nerve regeneration in patients with axonetmesis injuries. In this experiment on animals, Wistar rats’ damaged sensory systems were treated with lasers to restore them. Animals were randomly divided into six groups: a sham group, a control group, and four laser treatment groups(1st group: performed every day, 10 sessions; 2nd group: performed every 2 days, 10 sessions; 3rd group: performed every day, 20 sessions; and 4th group: performed every 2 days, 20 sessions). Sensory function was determined using the Semmes–Weinstein monofilament test, which was repeated after the surgical procedure. The results showed that the 20-session group had the best improvement, most closely resembling the group without sensory test damage. The histomorphometric results showed that the number of axons was significantly lower in the group that received 10 daily sessions and in the control group than in the undamaged nerve. Axon diameter was lower in all groups than in the sham group. In conclusion, the remarkable aspect of this study is that consecutive-day 20-session laser treatment showed better improvement than the over-the-day 20-session treatment protocol.

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Data availability

The datasets created and analyzed during the current study are available from the corresponding author upon request.


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This study was supported by the Nuh Naci Yazgan University Scientific Research Project Coordination Unit(2020-SA.DH-BP/15).

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Authors and Affiliations



K.O. contributed to study design, experimental procedures, data acquisition, analysis, and article writing. T. E. K. contributed to the design of the study, experimental phase, and manuscript revision. C. A. G. contributed to the interpretation of data and revised the manuscript. A.Y. and G.Ö.Ö contributed to the histological examination and interpretation of the tissues and obtained data.

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Correspondence to Kübra Öztürk.

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Ethical approval

All experimental protocols used in this study were approved by the Erciyes University Animal Experiments Local Ethics Committee, Kayseri, Turkey (approval no. 20/147).

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TYhe authors declare no competing interests

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Öztürk, K., Kuzu, T.E., Gürgan, C.A. et al. The effect of different treatment protocols with diode laser on regeneration in axonetmesis ınjuries of the ınferior alveolar nerve: an animal study. Lasers Med Sci 38, 169 (2023).

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