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Laser-photobiomodulation on titanium implant bone healing in rat model: comparison between 660- and 808-nm wavelength

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Abstract

Laser-photobiomodulation (L-PBM) has been widely studied and its biomodulatory effects have been established on irradiated cells, increasing viability and proliferation and on damaged tissues. In addition, L-PBM may reduce and modulate the inflammatory process. The effect of 660-nm and 808-nm laser-photobiomodulation on bone repair around titanium dental implants placed in rat’s femur was evaluated by histomorphometry. Twenty-seven Wistar rats were divided into 3 groups of nine animals: group C — non-irradiated control; group R — λ=660nm irradiated; and group IR — λ=808nm irradiated. Each group was further divided in 3 subgroups of three animals each, according to histomorphometry analysis in 3 days, 7 days, and 14 days after irradiation. Histological H.E.-stained slides were photographed, and bone matrix measured in new-formed bone area. Bone matrix histomorphometry analysis indicates that at 7 days in the irradiated groups (R and IR), a bigger area matrix was observed in relation to control group (C) (p=0.04 and p=0.048 respectively). On the other hand, at 14 days, control group (C) presented a bigger area than infrared irradiated (IR) (p=0.001) and red irradiated group (R) also showed a bigger area than infrared irradiated group (IR) (p=0.019). Histological analysis indicates that irradiated groups (R and IR) exhibited a faster bone tissue matrix production than control group

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Acknowledgements

We would like to thank DentoflexTM — Brazil that gently provided all the implant material used in this experiment.

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Authors and Affiliations

  1. Periodontology Program of Guarulhos University, praça Teresa Cristina 01, Guarulhos, São Paulo, SP, Cep 07023-070, Brazil

    Guilherme Aparecido Monteiro Duque da Fonseca, Juliana da Silva Pereira & Leandro augusto Pinto

  2. School of Dentistry, Nove de Julho University, Rua Vergueiro 235, São Paulo, SP, Cep 01504-001, Brazil

    Marcos Fernando Xisto Braga Cavalcanti

  3. Experimental Surgery Department of Pernambuco Federal University, Av. Prof. Moraes Rego, 1235 - Cidade Universitária, Recife, PE, Cep 50670-901, Brazil

    José Daniel de Souza Maior

  4. Implantology Program of Guarulhos University, praça Teresa Cristina 01, Guarulhos, São Paulo, Cep 07023-070, Brazil

    Murilo Matias & Lucio Frigo

Authors
  1. Guilherme Aparecido Monteiro Duque da Fonseca
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  2. Marcos Fernando Xisto Braga Cavalcanti
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  6. Murilo Matias
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Correspondence to Guilherme Aparecido Monteiro Duque da Fonseca or Lucio Frigo.

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This work was approved by the ethics committee of Universidade Federal de Pernambuco, Brazil, number: 23076.026402/2011-17 and respects laws of using animals for scientific purposes, in accordance with the ethical standards of the Helsinki Declaration of 1975 and revised in 2000.

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da Fonseca, G.A.M.D., Cavalcanti, M.F.X.B., de Souza Maior, J.D. et al. Laser-photobiomodulation on titanium implant bone healing in rat model: comparison between 660- and 808-nm wavelength. Lasers Med Sci 37, 2179–2184 (2022). https://doi.org/10.1007/s10103-021-03481-0

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  • DOI: https://doi.org/10.1007/s10103-021-03481-0

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