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High-frequency near-infrared diode laser irradiation suppresses IL-1β-induced inflammatory cytokine expression and NF-κB signaling pathways in human primary chondrocytes

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Abstract

Osteoarthritis (OA) and rheumatoid arthritis (RA) are common inflammation-associated cartilage degenerative diseases. Recent studies have shown that low-level diode laser treatment can reduce inflammatory cytokine expressions in cartilage. We recently reported that high-frequency low-level diode laser irradiation attenuates matrix metalloproteinases (MMPs) expression in human primary chondrocytes. However, the molecular mechanism underlying the effect of high-frequency low-level diode laser on chondrocytes remains unclear. Therefore, we aimed to elucidate the effect of high-frequency low-level diode laser irradiation on inflammatory cytokine expression in human primary chondrocytes. Normal human articular chondrocytes were treated with recombinant interleukin-1 beta (IL-1β) for 30 min or 24 h and irradiated with a high-frequency NIR diode laser at 8 J/cm2. The expression of IL-1β, interleukin-6, and tumor necrosis factor-alpha was assessed using western blot analysis. To evaluate the nuclear factor-kappa B (NF-κB) signaling pathway, the phosphorylation, translocation, and DNA-binding activity of NF-κB were detected using western blot analysis, immunofluorescence analysis, electrophoretic mobility shift assay, and enzyme-linked immunosorbent assay analysis. High-frequency low-level diode laser irradiation decreased inflammatory cytokine expression in IL-1β-treated chondrocytes. Moreover, high-frequency low-level diode laser irradiation decreased the phosphorylation, nuclear translocation, and DNA-binding activity of NF-κB in the IL-1β-treated state. However, irradiation alone did not affect NF-κB activity. Thus, high-frequency low-level diode laser irradiation at 8 J/cm2 can reduce inflammatory cytokine expressions in normal human articular chondrocytes through NF-κB regulation. These findings indicate that high-frequency low-level diode laser irradiation may reduce the expression of inflammatory cytokines in OA and RA.

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Acknowledgements

This work was carried out at the Research Center for Molecular Medicine, Hiroshima University, and was funded by grants from the JSPS KAKENHI (grant numbers: 20K18759, 20K10226, 19K10385, 18K17256, and 18K17257).

Funding

This work was supported by grants from the JSPS KAKENHI (grant numbers: 20K18759, 20K10226, 19K10385, 18K17256, and 18K17257).

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Authors and Affiliations

  1. Department of Orthodontics and Craniofacial Developmental Biology, Hiroshima University Graduate School of Biomedical and Health Sciences, 1-2-3 Kasumi, Minami-ku, Hiroshima, 734-8553, Japan

    Shuzo Sakata, Ryo Kunimatsu, Yuji Tsuka, Ayaka Nakatani, Hidemi Gunji, Makoto Yanoshita, Isamu Kado, Shota Ito, Nurul Aisyah Rizky Putranti, Rendra Chriestedy Prasetya, Naoto Hirose & Kotaro Tanimoto

  2. Department of Biomedical Faculty of Dentistry, University of Jember, Jember, Indonesia

    Shuzo Sakata, Ryo Kunimatsu, Yuji Tsuka, Ayaka Nakatani, Hidemi Gunji, Makoto Yanoshita, Isamu Kado, Shota Ito, Nurul Aisyah Rizky Putranti, Rendra Chriestedy Prasetya, Naoto Hirose & Kotaro Tanimoto

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  1. Shuzo Sakata
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  2. Ryo Kunimatsu
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Correspondence to Ryo Kunimatsu.

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Sakata, S., Kunimatsu, R., Tsuka, Y. et al. High-frequency near-infrared diode laser irradiation suppresses IL-1β-induced inflammatory cytokine expression and NF-κB signaling pathways in human primary chondrocytes. Lasers Med Sci 37, 1193–1201 (2022). https://doi.org/10.1007/s10103-021-03371-5

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  • DOI: https://doi.org/10.1007/s10103-021-03371-5

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