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The application of SFDI and LSI system to evaluate the blood perfusion in skin flap mouse model

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Abstract

The aim of this study is to evaluate whether the blood perfusion to tissues for detecting ischemic necrosis can be quantitatively monitored by spatial frequency domain imaging (SFDI) and laser speckle imaging (LSI) in a skin flap mouse model. Skin flaps were made on Institute of Cancer Research (ICR) mice. Using SFDI and LSI, the following parameters were estimated: oxyhemoglobin (HbO2), deoxyhemoglobin (Hb), total hemoglobin (THb), tissue oxygen saturation (StO2), and speckle flow index (SFI). Histologically, epithelium thickness, collagen deposition, and blood vessel count of skin flap tissues were analyzed. Then, the correlation of SFDI and histological results was assessed by application of Spearman rank correlation method. As the result, the number of blood vessels and the percentage of collagen areas showed significant difference between the necrotic tissue group and the non-necrotic one. Especially, the necrotic tissue had a complete epithelial loss and loses its normal structure. We identified that SFDI/LSI parameters were significantly different between non-necrotic and necrotic tissue groups. Especially, all SFDI and LSI parameters measured on the 1st day after surgery showed significant difference between necrotic tissue and non-necrotic tissue. In addition, the number of blood vessel and percentage of collagen area were positively correlated with HbO2 and StO2 among SFDI/LSI parameters. Meanwhile, the number of blood vessel and percentage of collagen area showed the negative correlation with Hb. By applying SFDI and LSI simultaneously to the skin flap, we could quantitatively monitor the blood perfusion and the tissue condition which can help us to detect ischemic necrosis objectively in early stage.

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Funding

This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (NRF-2017R1D1A1B03035027) and Leading Foreign Research Institute Recruitment Program through the NRF funded by the Ministry of Science and ICT (MSIT) (NRF-2018K1A4A3A02060572).

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Authors and Affiliations

  1. Department of Otorhinolaryngology, Dankook University College of Medicine, Cheonan, 31116, Republic of Korea

    Lele Lyu, Jun-Sang Bae, Cheng Hua, Jie Hye Kim, Eun-Hee Kim & Ji-Hun Mo

  2. Beckman Laser Institute Korea, Dankook University College of Medicine, Cheonan, 31116, Republic of Korea

    Lele Lyu, Hyeongbeom Kim, Jun-Sang Bae, Cheng Hua, Jie Hye Kim, Eun-Hee Kim, Ji-Hun Mo & Ilyong Park

  3. Department of Biomedical Engineering, Dankook University College of Medicine, Cheonan, 31116, Republic of Korea

    Hyeongbeom Kim & Ilyong Park

  4. Laser Translational Clinical Trial Center, Dankook University Hospital, Cheonan, 31116, Republic of Korea

    Ji-Hun Mo

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  1. Lele Lyu
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Correspondence to Ji-Hun Mo or Ilyong Park.

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All the experiments were approved by Dankook University Institutional Animal Care & Use Committee and were performed in compliance with the regulations (approval no. DKU-18–038).

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Lele Lyu and Hyeongbeom Kim contributed equally to this work.

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Lyu, L., Kim, H., Bae, JS. et al. The application of SFDI and LSI system to evaluate the blood perfusion in skin flap mouse model. Lasers Med Sci 37, 1069–1079 (2022). https://doi.org/10.1007/s10103-021-03354-6

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  • DOI: https://doi.org/10.1007/s10103-021-03354-6

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