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Photobiomodulation promotes neural regeneration when compared to simvastatin treatment in a sciatic nerve crush model

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Abstract

To determine whether the effects of photobiomodulation (PBM) were associated with the use of Simvastatin in the functional recovery from sciatic nerve in mice submitted to crush injury. Fifty Swiss mice (approximately 3 months old; average weight 40 g) were randomly divided into six groups: naive, sham, control, PBM (660 nm, 10 J/cm2; 30 mW; 0.6 J per day for 28 days; 0.06 cm2; 16.8 J total and 20 s), Simvastatin (20 mg/kg), and PBM/Simv (association of the two protocols). The sciatic functional index (SFI), thermal heat hyperalgesia, mechanical hyperalgesia, and thermographic evaluation were used as analyses. The evaluations were performed preoperatively and 7, 14, 21, and 28 days after the initial injury analyzed by two-way analysis of variance (ANOVA) for mixed models followed by the Bonferroni post-test. All groups except sham and naive presented an SFI compatible with severe peripheral nerve injury on the 7th day of evaluation. The PBM group presented better results in the SFI analysis (p < 0.001) on the 21st postoperative day compared to the control group. This benefit was maintained when compared to the Simvastatin (p < 0.001) and PBM/Simv groups (p < 0.01). The results of the thermal and mechanical hyperalgesia and thermography analyses were not significant (p > 0.05). The obtained results showed that PBM alone was more effective compared to Simvastatin alone or PBM combined with Simvastatin for sciatic nerve injury in mice.

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Funding

This study was financed in part by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior-Brasil (CAPES)-Finance Code 001 and had support from the Exercise Biology Laboratory (LABIOEX), from UFSC by Professor Aderbal Silva Aguiar Junior and Laboratory of Autoimmunity and Immunopharmacology (LAIF), from UFSC by Professor Rafael C. Dutra.

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Authors and Affiliations

  1. Postgraduate Program in Rehabilitation Sciences, Federal University of Santa Catarina/UFSC, Araranguá, Brazil

    Luana Gabriel de Souza, Ketlyn Germann Hendler, Alexandre Márcio Marcolino, Heloyse Uliam Kuriki, Ramon Bauer Cardoso & Rafael Inácio Barbosa

  2. Postgraduate Program in Functional Rehabilitation and Performance, University of São Paulo USP, Ribeirão Preto, Brazil

    Marisa de Cássia Registro Fonseca

  3. Laboratory of Assessment and Rehabilitation of Locomotor System, Federal University of Santa Catarina (LARAL/UFSC), Rua Pedro João Pereira, 150, Araranguá, Santa Catarina, 88905-120, Brazil

    Rafael Inácio Barbosa

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  1. Luana Gabriel de Souza
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  2. Ketlyn Germann Hendler
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  3. Alexandre Márcio Marcolino
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  4. Heloyse Uliam Kuriki
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  6. Marisa de Cássia Registro Fonseca
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  7. Rafael Inácio Barbosa
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Correspondence to Rafael Inácio Barbosa.

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All procedures were approved by the Animal Ethics Committee (CEUA/UFSC-protocol 8142240317).

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de Souza, L.G., Hendler, K.G., Marcolino, A.M. et al. Photobiomodulation promotes neural regeneration when compared to simvastatin treatment in a sciatic nerve crush model. Lasers Med Sci 36, 1591–1597 (2021). https://doi.org/10.1007/s10103-020-03176-y

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