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Combined effects of photobiomodulation and curcumin on mast cells and wound strength in wound healing of streptozotocin-induced diabetes in rats

Lasers in Medical Science volume 36, pages 375–386 (2021)Cite this article

Abstract

We investigated the probable involvement of mast cell degranulation and their numbers in the remodeling step of wound healing in a diabetic ischemic skin wound model treated with photobiomodulation plus curcumin. A total of 108 adult male Wistar rats were randomized into one healthy control and five diabetic groups. Type I diabetes was inflicted in 90 of the 108 rats. After 1 month, an excisional wound was generated in each of the 108 rats. There were one healthy group (group 1) and five diabetic groups as follows: group 2 was the untreated diabetic control group and group 3 rats were treated with sesame oil. Rats in group 4 were treated with photobiomodulation (890 nm, 890 ± 10 nm, 80 Hz, 0.2 J/cm2) and those in group 5 received curcumin dissolved in sesame oil. Group 6 rats were treated with photobiomodulation and curcumin. We conducted stereological and tensiometric tests on days 4, 7, and 15 after treatment. The results indicated that photobiomodulation significantly improved wound strength in the diabetic rats and significantly decreased the total numbers of mast cells. The diabetic control group had significantly reduced tensiometric properties of the healing wounds and a significant increase in the total numbers of mast cells. Photobiomodulation significantly improved the healing process in diabetic animals and significantly decreased the total number of mast cells. The increased numbers of mast cells in the diabetic control group negatively affected tensiometric properties of the ischemic skin wound.

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Affiliations

  1. Department of Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran

    Hasan Soleimani, Abdollah Amini, Mohammad-Amin Abdollahifar, Mohsen Norouzian & Mohammad Bayat

  2. Department of Anatomical Sciences, Zanjan University of Medical Sciences, Zanjan, Iran

    Reza Kouhkheil

  3. Department of Anatomy, Faculty of Medicine, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran

    Atarodsadat Mostafavinia

  4. Department of Statistics, University of Qom, Qom, Iran

    Seyed Kamran Ghoreishi

  5. Illinois Institute of Technology, Chicago, IL, USA

    Sahar Bayat

  6. Price Institute of Surgical Research, University of Louisville, Louisville, KY, USA

    Sufan Chien & Mohammad Bayat

  7. Noveratech LLC of Louisville, Louisville, KY, USA

    Sufan Chien & Mohammad Bayat

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  1. Hasan Soleimani
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  2. Abdollah Amini
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  3. Mohammad-Amin Abdollahifar
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  5. Reza Kouhkheil
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Soleimani, H., Amini, A., Abdollahifar, MA. et al. Combined effects of photobiomodulation and curcumin on mast cells and wound strength in wound healing of streptozotocin-induced diabetes in rats. Lasers Med Sci 36, 375–386 (2021). https://doi.org/10.1007/s10103-020-03053-8

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Keywords

  • Diabetes mellitus
  • Photobiomodulation
  • Curcumin
  • Mast cell
  • Histology
  • Tensiometric properties
  • Rat