Papacarie Duo™ is clinically used and has proven effectiveness; however, it is necessary to improve its antimicrobial action. The combined treatment of Papacarie Duo™ with Urucum (Bixa Orellana) could create a potential tool for dental caries treatment; its extract obtained from the seeds’ pericarp contains a water-soluble primary pigment (cis-bixin) with smaller amounts of other carotenoids. The dicarboxylic acid salts of cis-norbixin and trans-norbixin occur in heated alkaline solutions. To analyze the absorption spectra and cytotoxicity (with human dermal fibroblasts) in different concentrations of Urucum, associated or not with Papacarie Duo™, we performed this in vitro study. The effects of pure Urucum, Papacarie Duo™, and PapaUrucum™ on the microstructure of collagen were also analyzed. The application of papain-based gel with Urucum did not present cytotoxicity, its exhibit UV absorption spectrum peak around 460 ± 20 nm. Also, it showed that the compound used did not alter the chemical structure of collagen. Consequently, this product could be used as a chemomechanical method to remove dentin caries as well as being a potential product for antimicrobial photodynamic therapy (aPDT) application.
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The authors gratefully to Nove de Julho University to the technological support.
Z. Santos S. Jr. was finanacially supported by CAPES-Brazil grant 99999.002158/2014-00. M.R. Hamblin was financially supported by US NIH grant R01AI050875.
Conflict of interest
The authors declare non-financial interests concerning the work described. M.R. Hamblin is also a present member of the Transdermal Cap, Inc. scientific advisory board.
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Silva, Z.S., França, C.M., Araújo Prates, R. et al. The effects of photodynamic therapy with blue light and papain-based gel associated with Urucum, on collagen and fibroblasts: a spectroscopic and cytotoxicity analysis. Lasers Med Sci 35, 767–775 (2020). https://doi.org/10.1007/s10103-019-02857-7
- Papain-based gel
- Fourier transform infrared spectroscopy