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Photobiomodulation therapy compensate the impairments of diabetic bone marrow mesenchymal stem cells

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Abstract

Pathophysiologic conditions associated with diabetes mellitus affect mesenchymal stem cells (MSCs), and this phenomenon may lead to some diabetic secondary complications. The present study was conducted to evaluate the impact of photobiomodulation (PBM) on rat diabetic MSC (DMSC) behavior in vitro. For the purpose of PBM, we used helium-neon laser with a wavelength of 632.8 nm at three different energy densities (0.5, 1, 2 J/cm2) and radiation periodicity of once, twice, and thrice. The survival, proliferation, and apoptosis in the normal MSCs (NMSCs), DMSCs, and diabetic MSCs, which were laser irradiated (DMSCs+L), were assessed using MTT assay, Ki67 immunofluorescence staining, and TUNEL assay, respectively. Our results demonstrated that DMSCs have significantly lower survival (P < 0.05) and proliferation rates (P < 0.001), and dramatically higher population doubling time (PDT, P < 0.001) and apoptosis rates (P < 0.001) as compared to NMSCs. Moreover, PBM with energy density of 1 J/cm2 and the periodicity of 1 or 2 times could improve diabetic MSC capabilities in the term of survival, proliferation, and apoptosis. Considering these findings, it is suggested that PBM could improve the ability of diabetic MSCs in vitro prior to transplantation or may rise their capabilities in their native niche in vivo.

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Acknowledgments

The present article is based on the thesis by Fatemeh Zare at the School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran (Registration No. M64).

Funding

This study was supported by Regenerative Medicine and Stem Cell Research Network, Shahid Beheshti University of Medical Sciences, Tehran, Iran (Grant No. 7803).

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Authors and Affiliations

  1. Department of Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, 1985717443, Iran

    Fatemeh Zare, Mohammad Bayat & Abbas Aliaghaei

  2. Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran

    Abbas Piryaei

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  1. Fatemeh Zare
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  2. Mohammad Bayat
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  3. Abbas Aliaghaei
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Correspondence to Abbas Piryaei.

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Zare, F., Bayat, M., Aliaghaei, A. et al. Photobiomodulation therapy compensate the impairments of diabetic bone marrow mesenchymal stem cells. Lasers Med Sci 35, 547–556 (2020). https://doi.org/10.1007/s10103-019-02844-y

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  • DOI: https://doi.org/10.1007/s10103-019-02844-y

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