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Effect of low-level laser therapy and zoledronic acid on bone repair process

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Abstract

This study aimed to evaluate, through histomorphometric analysis, the bone repair process in the tibia of rats treated with zoledronic acid and submitted to 808-nm low-level laser therapy (LLLT) by using arsenide aluminum gallium laser. For this purpose, 20 rats were used and distributed according to treatment: group 1—saline administration; group 2—treated with LLLT; group 3—treated with zoledronic acid; and group 4—treated with zoledronic acid and LLLT. The zoledronic acid was administered at a dose of 0.035 mg/kg every 2 weeks for 8 weeks. Subsequently, bone defects of 2 mm were prepared in the tibias of all groups. The bone defects in groups 2 and 4 were irradiated with LLLT in the immediate post-operative period. After 14 and 28 days of application, the animals were submitted and euthanized for histomorphometric analysis. The results were submitted to statistical analysis (α = 5%), and the intragroup comparison was performed using the t test. On the other hand, for intergroup comparison, the ANOVA test was performed, and to the groups presenting statistically significant difference, the Student-Newman-Keuls test was used. In intergroup comparison, group 1 (mean ± SD= 45.2 ± 18.56%) showed a lower bone formation compared with groups 2 (64.13 ± 3.51%) (p = 0.358) and 4 (15.2 ± 78.22%) (p = 0.049), at the 14-day period. Group 3 (20.99 ± 7.42%) also presented a lower amount of neoformed bone tissue, with statistically significant difference when compared with groups 1 (p = 0.002), 2, and 4 (p ≤ 0,001). After 28 days, group 1 presented a lower amount of neoformed bone tissue compared with the other groups, with p = 0.020. Thus, it was concluded that LLLT associated with zoledronic acid is effective for stimulating bone formation in surgically created defects in rats, at the periods studied.

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Authors and Affiliations

  1. Oral Biology, Universidade do Sagrado Coração (USC), São Paulo, Brazil

    Vivian Chiacchio Buchignani & Evandro José Germano

  2. Endocrinology, Universidade Federal de São Paulo (Unifesp), São Paulo, Brazil

    Livia Marcela dos Santos

  3. Oral and Maxillofacial Surgery and Traumatology, Universidade do Sagrado Coração (USC), Bauru, SP, Brazil

    Jessica Lemos Gulinelli

  4. Oral Biology, Universidade de São Paulo (USP), Bauru, SP, Brazil

    Bella Luna Colombini Ishikiriama

  5. Biology, Universidade do Sagrado Coração (USC), São Paulo, Brazil

    Wilson Aparecido Orcini

  6. Department of Health Sciences, Universidade of Araraquara (UNIARA), Rua Carlos Gomes, 1338, Centro, Araraquara, SP, 14801-340, Brazil

    Luciana Monti Lima Rivera & Pâmela Leticia Santos

Authors
  1. Vivian Chiacchio Buchignani
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  2. Evandro José Germano
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  3. Livia Marcela dos Santos
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  4. Jessica Lemos Gulinelli
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  5. Bella Luna Colombini Ishikiriama
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  8. Pâmela Leticia Santos
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Contributions

Conceived and designed the experiments: PLS, LMS, JLG, BLCI, LMLR; performed the experiments: VCB, EJG, JLG, PLS; analyzed the data: WO, BLCI, LMLR, PLS; contributed reagents/materials/analysis tools: WO, VCB, EJG, LMS; wrote the paper: PLS, LMS, JLG.

Corresponding author

Correspondence to Pâmela Leticia Santos.

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The authors declare that they have no conflict of interest.

Ethical approval

All applicable international, national, and/or institutional guidelines for the care and use of animals were followed. All procedures performed in studies involving animals were in accordance with the ethical standards of the institution or practice at which the studies were conducted (attached supplementary materials—protocol number 006/13).

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The authors declare that it was not necessary to have informed consent since the research was not conducted in humans.

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Buchignani, V.C., Germano, E.J., dos Santos, L.M. et al. Effect of low-level laser therapy and zoledronic acid on bone repair process. Lasers Med Sci 34, 1081–1088 (2019). https://doi.org/10.1007/s10103-019-02810-8

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  • DOI: https://doi.org/10.1007/s10103-019-02810-8

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