Lasers in Medical Science

, Volume 34, Issue 6, pp 1081–1088 | Cite as

Effect of low-level laser therapy and zoledronic acid on bone repair process

  • Vivian Chiacchio Buchignani
  • Evandro José Germano
  • Livia Marcela dos Santos
  • Jessica Lemos Gulinelli
  • Bella Luna Colombini Ishikiriama
  • Wilson Aparecido Orcini
  • Luciana Monti Lima Rivera
  • Pâmela Leticia SantosEmail author
Original Article


This study aimed to evaluate, through histomorphometric analysis, the bone repair process in the tibia of rats treated with zoledronic acid and submitted to 808-nm low-level laser therapy (LLLT) by using arsenide aluminum gallium laser. For this purpose, 20 rats were used and distributed according to treatment: group 1—saline administration; group 2—treated with LLLT; group 3—treated with zoledronic acid; and group 4—treated with zoledronic acid and LLLT. The zoledronic acid was administered at a dose of 0.035 mg/kg every 2 weeks for 8 weeks. Subsequently, bone defects of 2 mm were prepared in the tibias of all groups. The bone defects in groups 2 and 4 were irradiated with LLLT in the immediate post-operative period. After 14 and 28 days of application, the animals were submitted and euthanized for histomorphometric analysis. The results were submitted to statistical analysis (α = 5%), and the intragroup comparison was performed using the t test. On the other hand, for intergroup comparison, the ANOVA test was performed, and to the groups presenting statistically significant difference, the Student-Newman-Keuls test was used. In intergroup comparison, group 1 (mean ± SD= 45.2 ± 18.56%) showed a lower bone formation compared with groups 2 (64.13 ± 3.51%) (p = 0.358) and 4 (15.2 ± 78.22%) (p = 0.049), at the 14-day period. Group 3 (20.99 ± 7.42%) also presented a lower amount of neoformed bone tissue, with statistically significant difference when compared with groups 1 (p = 0.002), 2, and 4 (p ≤ 0,001). After 28 days, group 1 presented a lower amount of neoformed bone tissue compared with the other groups, with p = 0.020. Thus, it was concluded that LLLT associated with zoledronic acid is effective for stimulating bone formation in surgically created defects in rats, at the periods studied.


Bisphosphonate Bone regeneration Laser therapy Rats 


Author contributions

Conceived and designed the experiments: PLS, LMS, JLG, BLCI, LMLR; performed the experiments: VCB, EJG, JLG, PLS; analyzed the data: WO, BLCI, LMLR, PLS; contributed reagents/materials/analysis tools: WO, VCB, EJG, LMS; wrote the paper: PLS, LMS, JLG.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

All applicable international, national, and/or institutional guidelines for the care and use of animals were followed. All procedures performed in studies involving animals were in accordance with the ethical standards of the institution or practice at which the studies were conducted (attached supplementary materials—protocol number 006/13).

Informed consent

The authors declare that it was not necessary to have informed consent since the research was not conducted in humans.


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Copyright information

© Springer-Verlag London Ltd., part of Springer Nature 2019

Authors and Affiliations

  • Vivian Chiacchio Buchignani
    • 1
  • Evandro José Germano
    • 1
  • Livia Marcela dos Santos
    • 2
  • Jessica Lemos Gulinelli
    • 3
  • Bella Luna Colombini Ishikiriama
    • 4
  • Wilson Aparecido Orcini
    • 5
  • Luciana Monti Lima Rivera
    • 6
  • Pâmela Leticia Santos
    • 6
    Email author
  1. 1.Oral BiologyUniversidade do Sagrado Coração (USC)São PauloBrazil
  2. 2.EndocrinologyUniversidade Federal de São Paulo (Unifesp)São PauloBrazil
  3. 3.Oral and Maxillofacial Surgery and TraumatologyUniversidade do Sagrado Coração (USC)BauruBrazil
  4. 4.Oral BiologyUniversidade de São Paulo (USP)BauruBrazil
  5. 5.BiologyUniversidade do Sagrado Coração (USC)São PauloBrazil
  6. 6.Department of Health SciencesUniversidade of Araraquara (UNIARA)AraraquaraBrazil

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