Lasers in Medical Science

, Volume 34, Issue 7, pp 1465–1472 | Cite as

Photobiomodulation therapy modulates epigenetic events and NF-κB expression in oral epithelial wound healing

  • Amanda de Farias Gabriel
  • Vivian Petersen Wagner
  • Cintia Correa
  • Liana Preto Webber
  • Emily Ferreira Salles Pilar
  • Marina Curra
  • Vinicius Coelho Carrard
  • Marco Antonio Trevizani Martins
  • Manoela Domingues MartinsEmail author
Original Article


The aim of this study was to evaluate the effect of photobiomodulation therapy (PBMT) on histone 3 acetylation (acH3) and NF-κB expression during oral ulcer healing. A total of 48 male Wistar rats were divided into control group (CG) and PBMT group (n = 24 each). Traumatic ulcers were created in the dorsum of the rats’ tongue with a punch tool. Irradiation with InGaAlP laser, 660 nm, 40 mW, 0.04 cm2 spot size, 4 J/cm2, 4 s, and 0.16 J per spot was performed once a day in close contact for 10 consecutive days. CG received only daily handling. Rats were euthanized on days 3, 5, and 10 (n = 8) and were monitored daily to assess wound status. Immunohistochemical analysis for acH3 and NF-κB detection was performed. One thousand epithelial cells were counted, and mean acH3- and NF-κB-positive cells were calculated and compared between the groups. PBMT accelerated the repair of oral ulcers. On day 3, PBMT showed significantly higher means for acH3- and NF-κB-positive cells than CG. On day 5, no difference was observed between the groups concerning both markers. On day 10, PBMT presented lower acH3 and NF-κB means than the control group. We concluded that PBMT stimulates keratinocyte migration in the early stage of oral wound healing and keratinocyte differentiation at the final stage by modulating histone acetylation and NF-κB expression.


Low-level laser therapy Diode laser Repair Histone acetylase enzyme Histones deacetylase enzyme 



The authors are grateful to Marta Justina Giotti Cioato and Flavia Rejane Giusti for technical support.

Funding information

This study was funded by the Postgraduate Research Group of Porto Alegre Clinics Hospital (GPPG/FIPE: 2014-0534), Brazilian National Council for Scientific and Technological Development (CNPq student scholarship), Children’s Cancer Institute (student scholarship), and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior-Brasil(CAPES)-finance code 001. Manoela Domingues Martins is a research fellow funded by the Brazilian National Council for Scientific and Technological Development (CNPq).

Compliance with ethical standards

The study was approved by the ethics committee of Porto Alegre Clinics Hospital (Brazil) under process number 14-0534. All applicable international, national, and/or institutional guidelines for the care and use of animals were followed. All procedures performed involving animals were in accordance with the ethical standards of the institution, which the study was conducted.

Conflict of interest

The authors declare that they have no conflict of interest.


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Copyright information

© Springer-Verlag London Ltd., part of Springer Nature 2019

Authors and Affiliations

  • Amanda de Farias Gabriel
    • 1
  • Vivian Petersen Wagner
    • 1
    • 2
  • Cintia Correa
    • 1
  • Liana Preto Webber
    • 1
    • 3
  • Emily Ferreira Salles Pilar
    • 4
  • Marina Curra
    • 5
  • Vinicius Coelho Carrard
    • 1
    • 6
  • Marco Antonio Trevizani Martins
    • 6
  • Manoela Domingues Martins
    • 1
    • 2
    • 4
    • 6
    Email author
  1. 1.Department of Oral Pathology, School of DentistryFederal University of Rio Grande do SulPorto AlegreBrazil
  2. 2.Department of Oral Diagnosis, Piracicaba Dental SchoolUniversity of CampinasPiracicabaBrazil
  3. 3.Laboratory of Epithelial Biology, Department of Periodontics and Oral MedicineUniversity of Michigan School of DentistryAnn ArborUSA
  4. 4.Experimental Pathology Unit, Clinics Hospital of Porto AlegreFederal University of Rio Grande do SulPorto AlegreBrazil
  5. 5.School of DentistryUniversity of Caxias do SulCaxias do SulBrazil
  6. 6.Department of Oral MedicinePorto Alegre Clinics Hospital (HCPA/UFRGS)Porto AlegreBrazil

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