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Effect of 650-nm low-level laser irradiation on c-Jun, c-Fos, ICAM-1, and CCL2 expression in experimental periodontitis

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A Correction to this article was published on 10 December 2019

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Abstract

This study was designed to investigate the effect of 650-nm low-level laser irradiation (LLLI) as an adjunctive treatment of experimental periodontitis. To investigate possible LLLI-mediated anti-inflammatory effects, we utilized an experimental periodontitis (EP) rat model and analyzed c-Jun, c-Fos, ICAM-1, and CCL2 gene expressions on PB leukocytes and in the gingival tissue. Total RNA was isolated from the gingivae and peripheral blood (PB) leukocytes of normal, EP, scaling, and root planing (SRP)-treated EP and LLLI + SRP-treated EP rats, and gene expressions were analyzed by real-time PCR. The productions of c-Jun, c-Fos, ICAM-1, and CCL2 in gingivae were analyzed immunohistochemically. Tartrate-resistant acid phosphatase (TRAP) staining was used to determine osteoclast activity in alveolar bone. The c-Jun and ICAM-1 messenger RNA (mRNA) levels were significantly decreased in the EP rat gingival tissue treated by SRP + LLLI than by SRP, the c-Jun, ICAM-1, and c-Fos mRNA levels on PB leukocytes reduced after LLLI treatment but did not show any significant differences in both groups. There was no significant difference in CCL2 mRNA levels on PB leukocytes and in gingivae between the SRP + LLLI and the SRP groups. The c-Fos mRNA levels in gingivae did not show significant difference in both groups. Immunohistochemistry showed that the CCL2, ICAM-1, c-Jun, and c-Fos productions were significantly reduced in rats of the SRP + LLLI group compared with the only SRP group. LLLI significantly decreased the number of osteoclasts as demonstrated by TRAP staining. The 650-nm LLLI might be a useful treatment modality for periodontitis.

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  • 10 December 2019

    After publication of our article [1] we realized that we had not acknowledged that some of the text overlaps with a previous publication [2]. We apologize to readers for this error.

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Acknowledgements

All authors were involved in drafting the article or revising it critically for important intellectual content, and all authors approved the final version to be published. Dr. Wang had full access to all of the data in the study and takes responsibility for the integrity of the data and the accuracy of the data analysis. We would like to acknowledge Jinbao Liu, Zhihe Dai, Yuzhu Mu, and Xiaomin Xu for their excellent technical assistances.

Funding

This study was supported by grants from the Natural Science Foundation of Dental School of Tianjin Medical University (No. 2014YKY03).

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Authors and Affiliations

  1. School of Dentistry, Hospital of Stomatology, Tianjin Medical University, 12 Qi Xiang Tai Street, Heping District, Tianjin, 300070, China

    Lin Zhang, Wenlei Chen, Gang Bao, Li Xiao, Pengfei Gao & Yonglan Wang

  2. Institute of Biomedical Engineering, Academy of Medical Science and Peking Union Medical College, Tianjin, China

    Yingxin Li & Xiaoxi Dong

  3. Department of Histology and Embryology, School of Basic Medical Sciences, Tianjin Medical University, Tianjin, China

    Wei Hong & Xiaohui Han

  4. Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Tianjin Medical University, Tianjin, China

    Haidong Li

  5. School of Public Health, Tianjin Medical University, Tianjin, China

    Zhuang Cui

Authors
  1. Lin Zhang
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  2. Wenlei Chen
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  4. Wei Hong
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  10. Li Xiao
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  12. Yonglan Wang
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Contributions

Study conception and design: Yonglan Wang.

Acquisition of data: Lin Zhang, Wenlei Chen, Haidong Li, Xiaoxi Dong, Xiaohui Han, Gang Bao, Li Xiao, Pengfei Gao.

Analysis and interpretation of data: Yonglan Wang, Lin Zhang, Yingxin Li, Wei Hong, Haidong Li.

Manuscript preparation: Lin Zhang.

Statistical analysis: Zhuang Cui, Lin Zhang, Haidong Li.

Corresponding author

Correspondence to Yonglan Wang.

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Zhang, L., Chen, W., Li, Y. et al. Effect of 650-nm low-level laser irradiation on c-Jun, c-Fos, ICAM-1, and CCL2 expression in experimental periodontitis. Lasers Med Sci 35, 31–40 (2020). https://doi.org/10.1007/s10103-018-2662-y

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  • DOI: https://doi.org/10.1007/s10103-018-2662-y

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