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Photobiomodulation on critical bone defects of rat calvaria: a systematic review

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Abstract

Bone defects following trauma represent a high impact on the quality of life of millions of people around the world. The aim of this study was to review photobiomodulation (PBM) action in the treatment of bone critical defects in rat calvaria, related to evaluation of the current protocols applied. One hundred and forty-seven articles related to the subject were found by searching the main databases (Pubmed, Lilacs, Web of Science, and Scopus) considering the period of publication until the year 2017, and only 14 corresponded the inclusion criteria established for this systematic review. The main parameters of the PBM were expressed in Table 1. In addition, it was possible to observe the use of two different wavelengths (red and infrared), which are considered therapeutic. Most of the evaluated articles presented positive results that describe a greater amount of neoformed bone, an increase in collagen synthesis, and a contribution to microvascular reestablishment. However, two studies report no effect on the repair process when the PBM was used. In addition, we observed considerable variations between the values of power, fluence, and total energy, which make it difficult to compare the results presented between the selected studies. It was possible to conclude that the infrared laser was more effective in positively stimulating the bone repair process of critical defects. Furthermore, a discrepancy was found in the parameter values used, which made it difficult to choose the best protocol for the treatment of this type of lesion.

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References

  1. Rosa AP, De Sousa LG, Regalo SCH, Issa JPM, Barbosa APA, Pitol DL et al (2012) Effects of the combination of low-level laser irradiation and recombinant human bone morphogenetic protein-2 in bone repair. Lasers Med Sci 27(5):971–977. https://doi.org/10.1002/lsm.22198

    Article  PubMed  Google Scholar 

  2. Garcia VG, Da Conceição JM, Fernandes LA, De Almeida JM, Nagata MJH, Bosco AF et al (2013) Effects of LLLT in combination with bisphosphonate on bone healing in critical size defects: a histological and histometric study in rat calvaria. Lasers Med Sci 28(2):407–414. https://doi.org/10.1007/s10103-012-1068-5

    Article  PubMed  Google Scholar 

  3. Kazancioglu HO, Erirganli S, Aydin MS (2013) Effects of laser and ozone therapies on bone healing in the calvarial defects. J Craniofac Surg 24:2141–2146

    Article  Google Scholar 

  4. Nagata MJH, Santinoni CS, Pola NM, De Campos N, Messora MR, Bomfim SRM et al (2013) Bone marrow aspirate combined with low-level laser therapy: a new therapeutic approach to enhance bone healing. J Photochem Photobiol B 121:6–14. https://doi.org/10.1016/j.jphotobiol.2013.01.013

    Article  CAS  PubMed  Google Scholar 

  5. Cunha MJS, Esper LA, Sbrana MC, De Oliveira PGFP, Do Valle AL, De Almeida ALPF (2013) Effect of low-level laser on bone defects treated with bovine or autogenous bone grafts: in vivo study in rat calvaria. Biomedical Research International. https://doi.org/10.1155/2014/104230

    Google Scholar 

  6. de Almeida ALPF, Medeiros IL, Cunha MJS, Sbrana MC, de Oliveira PGFP, Esper LA (2014) The effect of low-level laser on bone healing in critical size defects treated with or without autogenous bone graft: an experimental study in rat calvaria. Clin Oral Implants Res 25(10):1131–1136. https://doi.org/10.1111/clr.12239

    Article  PubMed  Google Scholar 

  7. Oliveira PC, Meireles GCS, dos Santos NR, de Carvalho CM, de Souza APC, dos Santos JN et al (2008) The use of light photobiomodulation on the treatment of second-degree burns: a histological study of a rodent model. Photomed Laser Surg 26(4):289–299. https://doi.org/10.1089/pho.2007.2148

    Article  PubMed  Google Scholar 

  8. Garcia VG, Sahyon AS, Longo M, Fernandes LA, Gualberto Junior EC, Novaes VCN et al (2014) Effect of LLLT on autogenous bone grafts in the repair of critical size defects in the calvaria of immunosuppressed rats. J Cranio-Maxillofac Surg 42(7):1196–1202. https://doi.org/10.1016/j.jcms.2014.02.008

    Article  Google Scholar 

  9. Marques L, Holgado LA, Francischone LA, Ximenez JPB, Okamoto R, Kinoshita A (2015) New LLLT protocol to speed up the bone healing process—histometric and immunohistochemical analysis in rat calvarial bone defect. Lasers Med Sci 30(4):1225–1230. https://doi.org/10.1007/s10103-014-1580-x

    Article  PubMed  Google Scholar 

  10. Scalize PH, de Sousa LG, Regalo SCH, Semprini M, Pitol DL, da Silva GA et al (2015) Low-level laser therapy improves bone formation: stereology findings for osteoporosis in rat model. Lasers Med Sci 30(5):1599–1607. https://doi.org/10.1007/s10103-015-1773-y

    Article  PubMed  Google Scholar 

  11. Bosco AF, Faleiros PL, Carmona LR, Garcia VG, Theodoro LH, de Araujo NJ et al (2016) Effects of low-level laser therapy on bone healing of critical-size defects treated with bovine bone graft. J Photochem Photobiol B 163:303–310. https://doi.org/10.1016/j.jphotobiol.2016.08.040

    Article  CAS  PubMed  Google Scholar 

  12. Moreira GS, Henry P, Alves M, et al. (2018) Effect of low-level laser on the healing of bone defects filled with autogenous bone or bioactive glass: in vivo study. Int J Oral Maxillof Impants, 169–74. doi:https://doi.org/10.1002/lsm.22198

    Article  Google Scholar 

  13. Khadra M, Kasem N, Haanaes HR, Ellingsen JE, Lyngstadaas SP (2004) Enhancement of bone formation in rat calvarial bone defects using low-level laser therapy. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 97(6):693–700. https://doi.org/10.1016/j.tripleo.2003.11.008

    Article  PubMed  Google Scholar 

  14. Ã LC, Willie B (2007) The enhancement of bone regeneration by ultrasound, 93:384–98. doi:https://doi.org/10.1016/j.pbiomolbio.2006.07.021

    Article  Google Scholar 

  15. Fernandes KR, Ribeiro DA, Rodrigues NC, Tim C, Santos AA, Parizotto NA, et al. (2013) Effects of low-level laser therapy on the expression of osteogenic genes related in the initial stages of bone defects in rats. J Biomed Opt, 18(3). doi: https://doi.org/10.1117/1.JBO.18.3.038002

    Article  Google Scholar 

  16. Acar AH, Yolcu Ü, Altındiş S, Gül M, Alan H, Malkoç S (2016) Bone regeneration by low-level laser therapy and low-intensity pulsed ultrasound therapy in the rabbit calvarium. Arch Oral Biol 61:60–65. https://doi.org/10.1016/j.archoralbio.2015.10.011

    Article  PubMed  Google Scholar 

  17. Bossini PS, ACM R, Ribeiro DA, Fangel R, Ribeiro AC, Lahoz MA et al (2011) Low level laser therapy (830nm) improves bone repair in osteoporotic rats: similar outcomes at two different dosages. J Photochem Photobiol B. https://doi.org/10.1016/j.exger.2011.11.005

    Article  Google Scholar 

  18. Garavello-Freitas I, Baranauskas V, Joazeiro PP, Padovani CR, Dal Pai-Silva M, da Cruz MA (2003) Low-power laser irradiation improves histomorphometrical parameters and bone matrix organization during tibia wound healing in rats. Exp Gerontol 70(2):81–89

    CAS  Google Scholar 

  19. Liu WC, Chen S, Zheng L, Qin L. (2017) Angiogenesis assays for the evaluation of angiogenic properties of orthopaedic biomaterials—a general review. Advanced Healthc Mater, 1600434. doi: https://doi.org/10.1002/adhm.201600434

    Article  Google Scholar 

  20. Renno ACM, McDonnell PA, Parizotto NA, Laakso E-L (2007) The effects of laser irradiation on osteoblast and osteosarcoma cell proliferation and differentiation in vitro. Photomed Laser Surg 25(4):275–280. https://doi.org/10.1089/pho.2007.2055

    Article  CAS  PubMed  Google Scholar 

  21. Choi K, Kang B-J, Kim H, Lee S, Bae S, Kweon O-K et al (2013) Low-level laser therapy promotes the osteogenic potential of adipose-derived mesenchymal stem cells seeded on an acellular dermal matrix. J Biomed Mater Res B Appl Biomater 101:919–928. https://doi.org/10.1002/jbm.b.32897

    Article  CAS  PubMed  Google Scholar 

  22. Oliveira AM De, Castro-silva II, Vicentis G, Fernandes DO, Melo BR, Terezinha A, et al. (2014) Effectiveness and acceleration of bone repair in critical-sized rat calvarial defects using low-level laser therapy, 67(October 2013):61–7. doi: https://doi.org/10.1002/lsm.22198

    Article  Google Scholar 

  23. Hoffman T, Zancan R, De OL, Claudia A, Claudino M, Fernando A et al (2017) Effects of low-level laser therapy and platelet concentrate on bone repair : histological, histomorphometric, immunohistochemical, and radiographic study. Journal Craniomaxillofacial Surgery 45:1846–1853. https://doi.org/10.1016/j.jcms.2017.08.008

    Article  Google Scholar 

  24. Karu T (2010) Mitochondrial mechanisms of photobiomodulation in context of new data about multiple roles of ATP. Photomed Laser Surg 28(2):159–160. https://doi.org/10.1089/pho.2010.2789

    Article  CAS  PubMed  Google Scholar 

  25. Stein A, Benayahu D, Maltz L, Oron U (2005) Low-level laser irradiation promotes proliferation and differentiation of human osteoblasts in vitro. Photomed Laser Surg 23(2):161–166. https://doi.org/10.1089/pho.2005.23.161

    Article  CAS  PubMed  Google Scholar 

  26. Damante CA, De Micheli G, Miyagi SPH, Feist IS, Marques MM (2009) Effect of laser phototherapy on the release of fibroblast growth factors by human gingival fibroblasts. Lasers Med Sci 24(6):885–891. https://doi.org/10.1007/s10103-008-0582-y

    Article  PubMed  Google Scholar 

  27. da Silva RV, Camilli JA (2006) Repair of bone defects treated with autogenous bone graft and low-power laser. J Craniofac Surg 17(2):297–301

    Article  Google Scholar 

Download references

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This work is financially supported by the CAPES National Postdoctoral Program (PNPD/CAPES).

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Correspondence to Patricia Brassolatti.

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Brassolatti, P., de Andrade, A.L.M., Bossini, P.S. et al. Photobiomodulation on critical bone defects of rat calvaria: a systematic review. Lasers Med Sci 33, 1841–1848 (2018). https://doi.org/10.1007/s10103-018-2653-z

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  • DOI: https://doi.org/10.1007/s10103-018-2653-z

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