Visualize and quantify the structural alteration of the rat spinal cord injury based on multiphoton microscopy
- 101 Downloads
The development of imaging technique to visualize and quantify the structural alteration of the spinal cord injury (SCI) may lead to better understanding and treatments of the injuries. In this work, multiphoton microscopy (MPM) based on two-photon excited fluorescence (TPEF) and second-harmonic generation (SHG) was tentatively applied to quantitatively visualize the cellular microstructures of SCI to demonstrate the feasibility and superiority of MPM in SCI imaging. High-contrast MPM images of normal and injured spinal cord tissue were obtained for comparison. Moreover, the changes of injured spinal cord were characterized by the quantitative analysis of the MPM images. These results showed that MPM combined with quantitative method has the ability to identify the characteristics of spinal cord injury including the changes in the contents of nerve fibers and extracellular matrix. With the advancement of MPM, we believe that this technique has great potential to provide the histological diagnosis for the monitoring and evaluation of SCI.
KeywordsMultiphoton microscopy (MPM) Two-photon excited fluorescence (TPEF) Second-harmonic generation (SHG) Spinal cord injury (SCI)
The work was supported by the Changjiang Scholars Program and the University Innovative Research Team (grant no. IRT_15R10), the National Natural Science Foundation of China (grant nos. 81671730), the Natural Science Foundation of Fujian Province (2015J01241), and a grant from the Education Bureau of Fujian Province (grant no. JA13060).
Compliance with ethical standards
Conflict of interest
The authors declare that they have no competing interests.
The experimental procedures with rats were conducted according to the Guide for Care and Use of Laboratory Animals and approved by the Animal Care and Use Committee in Fujian Medical University.
It does not apply, since the study was developed with Sprague-Dawley rats.
- 11.Uckermann O, Galli R, Beiermeister R, Sitoci-Ficici KH, Later R, Leipnitz E, Neuwirth A, Chavakis T, Koch E, Schackert G, Steiner G, Kirsch M (2015) Endogenous two-photon excited fluorescence provides label-free visualization of the inflammatory response in the rodent spinal cord. Biomed Res Int 2015:859084CrossRefGoogle Scholar
- 24.Johansen-Berg H, Behrens TE (2013) Diffusion MRI: from quantitative measurement to in vivo neuroanatomy. Academic press, San DiegoGoogle Scholar
- 30.Helmchen F, Denk W, Kerr JN (2013) Miniaturization of two-photon microscopy for imaging in freely moving animals. Cold Spring Harb Protoc 2013:904–913Google Scholar