Comparative effect of photobiomodulation associated with dexamethasone after sciatic nerve injury model
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To analyze the effect of photobiomodulation and dexamethasone on nerve regeneration after a sciatic nerve crushing model. Twenty-six Swiss mice were divided into the following groups: naive; sham; injured, low-level laser therapy (LLLT) (660 nm, 10 J/cm2, 0.6 J, 16.8 J total energy emitted during the 28 days of radiation, 20 s, for 28 days); dexamethasone (Dex) (local injection of 2 mg/kg for 10 consecutive days); and LLLT group associated with Dex (LLLT/Dex), with the same parameters of the other groups. For nerve injury, a portable adjustable pinch was used. The animals were evaluated using the Sciatic Functional Index (SFI) and Sciatic Static Index (SSI). The results obtained were evaluated with Image J™ and Kinovea™. Data and images were obtained at baseline and after 7, 14, 21, and 28 days after surgery. The evaluation of hyperalgesia, using Hargreaves, and behavior through the open field was also performed. In functional and static analysis, all groups presented significant differences when compared to the injured group. In the analysis of the SSI results, the group treated with both LLLT and dexamethasone was more effective in improving the values of this parameter, and in the SFI, the laser-treated group obtained better results. In the evaluation through the open field and the Hargreaves, there was no difference. The application of LLLT and dexamethasone was effective in nerve regeneration according to the results and was more effective when LLLT was associated with dexamethasone than in LLLT alone for the SSI.
KeywordsSciatic nerve Nerve regeneration Crush injury Low-level laser therapy Dexamethasone
The authors would like to thank the CNPQ (National Research and Technology Development Council, Brazil), Rafael Cypriano Dutra (Laboratory of Autoimmunity and Immunopharmacology of Federal University of Santa Catarina, Brazil), and Nilton Mazzer (University of São Paulo, Brazil) for the support of this research.
Role of funding source
This project have had financial support from the Laboratory of Autoimmunity and Immunopharmacology of Federal University of Santa Catarina and State of São Paulo Research Foundation (Fundação de Amparo à Pesquisa do Estado de São Paulo—FAPESP), Brazil.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
All procedures were approved by the Animal Research Ethics Committee of Federal University of Santa Catarina (protocol no. PP00956).
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