Lasers in Medical Science

, Volume 33, Issue 7, pp 1441–1446 | Cite as

Effects of different treatments on chemical and morphological features of eroded dentin

  • Sandra Kiss Moura
  • Carolina Cury Lopes
  • Livia Tosi Trevelin
  • Adriana Bona Matos
  • Íssis Virgínia Luque Martinez
  • Marcelo Estevam
  • Sandra Kalil Bussadori
Original Article


To evaluate the treatment of eroded dentin (Sensodyne Repair & Protect™, Er:YAG laser and combinations). The occlusal surfaces of 25 third molars were sectioned 1.5 mm in thickness. After an erosion cycle (5 min in demineralizing solution + 3 h in remineralizing solution; six cycles a day for 8 days), the samples were divided into five groups (n = 5): (E) erosion − control; (ES) erosion + Sensodyne Repair & Protect (NovaMin); (EL) erosion + Er:YAG laser (40 mJ, 10 Hz, 0.4 W, 50 μs, 3.1 J/cm2, 63 W/cm2); (ELS) erosion + Er:YAG laser + Sensodyne; and (ESL) erosion + Sensodyne + Er:YAG laser. Following storage in ultrapure water (37 °C/14 days), the Ca/P ratio was evaluated by EDXRF and the morphology surfaces examined in SEM. The percentage of exposed dentin tubules was calculated. One-way analysis of variance and Tukey’s test at 5% were used to treat the data. The Ca/P ratio was higher in E and ES groups. More exposed dentin tubules were found in E group and less exposed tubules were found in the ES group (p < 0.0001). When the toothpaste and laser were combined, the number of occluded dentin tubules was higher when laser was performed first (ELS). A positive effect was found when the laser and toothpaste were combined.


Dental erosion Laser Desensitizing agents 


Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethics approval

Human third molars used in this study following approval from the human research ethics committee (certificate number: 1.209.605).


  1. 1.
    Moynihan PJ (2005) The role of diet and nutrition in the etiology and prevention of oral diseases. Bull World Health Organ 83:694–699PubMedPubMedCentralGoogle Scholar
  2. 2.
    Bartlett DW, Shah P (2006) A critical review of non-carious cervical (wear) lesions and the role of abfraction, erosion, and abrasion. J Dent Res 85:306–312CrossRefPubMedGoogle Scholar
  3. 3.
    Lopes AO, Aranha AC (2013) Comparative evaluation of the effects of Nd:YAG laser and a desensitizer agent on the treatment of dentin hypersensitivity: a clinical study. Photomed Laser Surg 31:132–138CrossRefPubMedPubMedCentralGoogle Scholar
  4. 4.
    Dantas EM, Dantas PMC, Nóbrega FGO, Vasconcellos RG, Aguiar Junior JN, Queiroz LMG (2013) Low-level laser therapy for treating cervical dentinal hypersensitivity—literature review. Odontol Clín Cient 12:7–11Google Scholar
  5. 5.
    Hashim NT, Gasmalla BG, Sabahelkheir AH, Awooda AM (2014) Effect of the clinical application of the diode laser (810 nm) in the treatment of dentine hypersensitivity. BMC Res Notes 7:31CrossRefPubMedPubMedCentralGoogle Scholar
  6. 6.
    Yu CH, Chang YC (2013) Clinical efficacy of the Er:YAG laser treatment on hypersensitive dentin. J Form Med Assoc 113:388–391CrossRefGoogle Scholar
  7. 7.
    Tunar OL, Gursoy H, ÇaKar G, Kuru B (2014) Evaluation of the effects of Er:YAG laser and desensitizing paste containing 8% arginine and calcium carbonate, and their combinations on human dentine tubules: a scanning electron microscopic analysis. Photomed Laser Surg 32:540–545CrossRefPubMedGoogle Scholar
  8. 8.
    Namour A, Nammour S, Peremans A, Heysselaer D, De Moor JG (2014) Treatment of dentinal hypersensitivity by means of Nd:YAP laser: a preliminary in vitro study. Sci World J 1:323304Google Scholar
  9. 9.
    Dilber E, Malkoc LA, Ozturk AN, Ozturk F (2013) Effect of various laser irradiations on the mineral content of dentin. Eur J Dent 7:74–80PubMedPubMedCentralGoogle Scholar
  10. 10.
    Belal MH, Yassin A (2014) A comparative evaluation of CO2 and erbium-doped yttrium aluminium garnet laser therapy in the management of dentin hypersensitivity and assessment of mineral content. J Periodontal Implant Sci 44:227–234CrossRefPubMedPubMedCentralGoogle Scholar
  11. 11.
    Han SY, Jung HI, Know HK, Kim BI (2013) Combined effects of Er:YAG laser and nano-carbonate apatite dentifrice on dentinal tubule occlusion: in vitro study. Photomed Laser Surg 31:342–348CrossRefPubMedGoogle Scholar
  12. 12.
    Wang Z, Sa Y, Sauro S, Chen H, Xing W (2010) Effect of desensitising toothpastes on dentinal tubule occlusion: a dentine permeability measurement and SEM in vitro study. J Dent 38:400–410CrossRefPubMedGoogle Scholar
  13. 13.
    Wefel JS (2009) NovaMin®: likely clinical success. Adv Dent Res 21:40–43CrossRefPubMedGoogle Scholar
  14. 14.
    Farmakis ET, Kozyrakis K, Khabbaz MG, Schoop U, Beer F, Morit A (2012) In vitro evaluation of dentin tubule occlusion by Denshield and neodymium-doped yttrium-aluminum-garnet laser irradiation. J Endod 38:662–666CrossRefPubMedGoogle Scholar
  15. 15.
    Trevelin LT, Marques MM, Aranha AC, Arana-Chavez VE, Matos AB (2015) Effect of super short pulse Er:YAG laser on human dentin: scanning electron microscopy analysis. Microsc Res Tech 78:472–478CrossRefPubMedGoogle Scholar
  16. 16.
    Zimmerli B, De Munck J, Lussi A, Lambrechts P (2012) Long-term bonding to eroded dentin requires superficial bur preparation. Clin Oral Invest 16:1451–1461CrossRefGoogle Scholar
  17. 17.
    Aranha AC, Pimenta LA, March GM (2009) Clinical evaluation of desensitizing treatments for cervical dentin hypersensitivity. Braz Oral Res 23:333–339CrossRefPubMedGoogle Scholar
  18. 18.
    Hibst R, Keller U (1989) Experimental studies of the application of the Er:YAG laser on dental hard substances. I. Measurement of the ablation rate. Lasers Surg Med 9:338–344CrossRefPubMedGoogle Scholar
  19. 19.
    Zhao-Zhang L, Code JE, Van de Merwe WP (1992) Er:YAG laser ablation of enamel and dentin of human teeth: determination of ablation rates at various fluences and pulse repetition rates. Lasers Surg Med 12:625–630CrossRefGoogle Scholar
  20. 20.
    Zapletalova Z, Perina JR, Novotny R, Chmelikhova H (2007) Suitable conditions for sealing of open dentinal tubules using a pulsed Nd:YAG laser. Photomed Laser Surg 25:495–499CrossRefPubMedGoogle Scholar
  21. 21.
    João-Souza SH, Scaramucci T, Hara AT, Aranha AC (2015) Effect of Nd:YAG laser irradiation and fluoride application in the progression of dentin erosion in vitro. Lasers Med Sci 30:2273–2279CrossRefPubMedGoogle Scholar
  22. 22.
    Jelínková H, Dostálová T, Dolezalová L, Krejsa O, Hamal K, Kubelka J, Procházka S (1997) Comparison of preparation speed of En YAG laser and conventional drilling machine. SPIE 2973:2–10Google Scholar
  23. 23.
    Badran Z, Boutigny H, Struillou X, Baroth S, Laboux O, Assem S (2011) Tooth desensitization with an Er:YAG laser: in vitro microscopical observation and a case report. Lasers Med Sci 26:139–142CrossRefPubMedGoogle Scholar
  24. 24.
    Milleman JR, Milleman KR, Clarck CE, Mongiello KA, Simonton TC, Proskin HM (2015) NUPRO Sensodyne prophylaxis paste with NovaMin for the treatment of dentin hypersensitivity: a 4-week clinical study. Am J Dent 25:262–268Google Scholar
  25. 25.
    Altundasar E, Ozcelik B, Cehreli ZC, Matsumoto K (2006) Ultramorphological and histochemical changes after Er,Cr:YSGG laser irradiation and two different irrigation regimes. J Endod 32:465–468CrossRefPubMedGoogle Scholar
  26. 26.
    Ari H, Erdemir A (2005) Effects of endodontic irrigation solutions on mineral content of root canal dentin using ICP-AES technique. J Endod 31:187–189CrossRefPubMedGoogle Scholar
  27. 27.
    Menezes RF, Harvey CM, Gerbi MEMM, Smith ZJ, Smith D, Ivaldi JC, Philips A, Chan JW, Wachsmann-Hogiu S (2017) Fs-laser ablation of teeth in temperature limited and provides information about the ablated components. J Biophotonics 10:1292–1304CrossRefPubMedGoogle Scholar
  28. 28.
    Chung SH, Mazur E (2017) Surgical applications of femtoseconds laser. J Biophotonics 2:557–572CrossRefGoogle Scholar

Copyright information

© Springer-Verlag London Ltd., part of Springer Nature 2018

Authors and Affiliations

  • Sandra Kiss Moura
    • 1
    • 2
  • Carolina Cury Lopes
    • 1
  • Livia Tosi Trevelin
    • 3
  • Adriana Bona Matos
    • 3
  • Íssis Virgínia Luque Martinez
    • 4
  • Marcelo Estevam
    • 5
  • Sandra Kalil Bussadori
    • 2
  1. 1.Department of DentistryUniversity North of Parana (UNOPAR)LondrinaBrazil
  2. 2.Department of Biophotonics Applied to Health SciencesNove de Julho University (UNINOVE)São PauloBrazil
  3. 3.Department of DentistryUniversity of São Paulo (FOUSP)São PauloBrazil
  4. 4.Dentistry Academic Unit, Faculty of MedicinePontificia Universidad Católica de ChileSantiagoChile
  5. 5.LPIS Health Research and Innovation LaboratoryFederal Institute of Parana (IFPR)LondrinaBrazil

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