Lasers in Medical Science

, Volume 33, Issue 6, pp 1189–1195 | Cite as

Effects of 915 nm laser irradiation on human osteoblasts: a preliminary in vitro study

  • Giovanni Mergoni
  • Paolo Vescovi
  • Silvana Belletti
  • Jacopo Uggeri
  • Samir Nammour
  • Rita Gatti
Original Article


Photobiomodulation (PBM) is a non-invasive treatment that uses laser or led devices making its effects a response to light and not to heat. The possibility of accelerating dental implant osteointegration and orthodontic movements and the need to treat refractory bone lesions, such as bisphosphonate related osteonecrosis of the jaws, has led researchers to consider the effects of PBM on bone for dentistry purposes. The aim of our study was to investigate the effects of 915 nm light supplied with a GaAs diode laser on human osteoblasts in vitro. Osteoblasts were isolated from mandibular cortical bone of a young healthy donor. The irradiation parameters were as follows: doses = 5, 15 and 45 J/cm2; power densities = 0.12 and 1.25 W/cm2; and irradiation times = 41.7, 125 and 375 s. We performed one irradiation per day for 3 and 6 days to study proliferation and differentiation, respectively. Microscopic analysis showed a greater amount of bone nodules in samples treated with 5 J/cm2 and 0.12 W/cm2 compared to controls (56.00 ± 10.44 vs 19.67 ± 7.64, P = 0.0075). Cell growth and quantification of calcium deposition did not show any differences when comparing irradiated and non-irradiated samples. Photobiomodulation, with the parameters investigated in the present study, positively modulated the mineralization process in human osteoblasts, inducing the formation of a greater amount of bone nodules, but did not increase cell proliferation.


Photobiomodulation Diode laser Osteoblast Proliferation Differentiation 


Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki Declaration and its later amendments or comparable ethical standards.


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Copyright information

© Springer-Verlag London Ltd., part of Springer Nature 2018

Authors and Affiliations

  1. 1.Unit of Oral Pathology and Laser-assisted Oral Surgery; Department of Medicine and SurgeryUniversity of ParmaParmaItaly
  2. 2.Unit of Anatomy Histology and Embryology, Department of Medicine and SurgeryUniversity of ParmaParmaItaly
  3. 3.Unità Operativa di Patologia ClinicaOspedale di VaioFidenzaItaly
  4. 4.Department of Dental Sciences, Faculty of MedicineUniversity of LiègeLiegeBelgium

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