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High final energy of gallium arsenide laser increases MyoD gene expression during the intermediate phase of muscle regeneration after cryoinjury in rats

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Abstract

The aim of this study was to determine the effects of gallium arsenide (GaAs) laser on IGF-I, MyoD, MAFbx, and TNF-α gene expression during the intermediate phase of muscle regeneration after cryoinjury 21 Wistar rats were divided into three groups (n = 7 per group): untreated with no injury (control group), cryoinjury without GaAs (injured group), and cryoinjury with GaAs (GaAs-injured group). The cryoinjury was induced in the central region of the tibialis anterior muscle (TA). The region injured was irradiated once a day during 14 days using GaAs laser (904 nm; spot size 0.035 cm2, output power 50 mW; energy density 69 J cm−2; exposure time 4 s per point; final energy 4.8 J). Twenty-four hours after the last application, the right and left TA muscles were collected for histological (collagen content) and molecular (gene expression of IGF-I, MyoD, MAFbx, and TNF-α) analyses, respectively. Data were analyzed using one-way ANOVA at P < 0.05. There were no significant (P > 0.05) differences in collagen density and IGF-I gene expression in all experimental groups. There were similar (P < 0.05) decreases in MAFbx and TNF-α gene expression in the injured and GaAs-injured groups, compared to control group. The MyoD gene expression increased (P = 0.008) in the GaAs-injured group, but not in the injured group (P = 0.338), compared to control group. GaAs laser therapy had a positive effect on MyoD gene expression, but not IGF-I, MAFbx, and TNF-α, during intermediary phases (14 days post-injury) of muscle repair.

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Funding

We thank the Institutional Program of Scientific Initiation Scholarships (PIBIC) from the National Council for Scientific and Technological Development (CNPq) for financial support (Process No. 146045/2013–0).

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Authors and Affiliations

  1. Department of Physical Therapy, University of Western São Paulo (UNOESTE), Presidente Prudente, São Paulo, Brazil

    Caroline Pereira Santos, Ines Cristina Giometti, Thaoan Bruno Mariano, Carlos Eduardo Assumpção de Freitas, Gisele Alborghetti Nai, Selma Zambelli de Freitas & Francis Lopes Pacagnelli

  2. Center of Research in Health Science, North University of Paraná (UNOPAR), Avenue Paris, 675, Jardim Piza, Londrina, PR, 86041-120, Brazil

    Andreo Fernando Aguiar

  3. Department of Morphology, São Paulo State University (UNESP), Botucatu, São Paulo, Brazil

    Maeli Dal Pai-Silva

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  1. Caroline Pereira Santos
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Correspondence to Andreo Fernando Aguiar.

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All procedures were approved by the Ethics Research Committee of the University of Western São Paulo (UNOESTE), Presidente Prudente, São Paulo, Brazil (Protocol No. 713).

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Santos, C.P., Aguiar, A.F., Giometti, I.C. et al. High final energy of gallium arsenide laser increases MyoD gene expression during the intermediate phase of muscle regeneration after cryoinjury in rats. Lasers Med Sci 33, 843–850 (2018). https://doi.org/10.1007/s10103-018-2439-3

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