Lasers in Medical Science

, Volume 33, Issue 3, pp 609–617 | Cite as

In vivo study of dermal collagen of striae distensae by confocal Raman spectroscopy

  • Pam Wen Lung
  • Vamshi Krishna Tippavajhala
  • Thiago de Oliveira Mendes
  • Claudio A. Téllez-Soto
  • Desirée Cigaran Schuck
  • Carla Abdo Brohem
  • Marcio Lorencini
  • Airton Abrahão Martin
Original Article


This research work mainly deals with studying qualitatively the changes in the dermal collagen of two forms of striae distensae (SD) namely striae rubrae (SR) and striae albae (SA) when compared to normal skin (NS) using confocal Raman spectroscopy. The methodology includes an in vivo human skin study for the comparison of confocal Raman spectra of dermis region of SR, SA, and NS by supervised multivariate analysis using partial least squares discriminant analysis (PLS-DA) to determine qualitatively the changes in dermal collagen. These groups are further analyzed for the extent of hydration of dermal collagen by studying the changes in the water content bound to it. PLS-DA score plot showed good separation of the confocal Raman spectra of dermis region into SR, SA, and NS data groups. Further analysis using loading plot and S-plot indicated the participation of various components of dermal collagen in the separation of these groups. Bound water content analysis showed that the extent of hydration of collagen is more in SD when compared to NS. Based on the results obtained, this study confirms the active involvement of dermal collagen in the formation of SD. It also emphasizes the need to study quantitatively the role of these various biochemical changes in the dermal collagen responsible for the variance between SR, SA, and NS.


Confocal Raman spectroscopy Human dermal collagen Striae distensae In vivo human skin study 



Authors thank FVE, Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) (307809/2013-7) and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) (project number 88881.068140/2014-01) for their financial support.

Funding information

This study is funded by Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) with the project numbers 307809/2013-7 and 88881.068140/2014-01, respectively.

Compliance with ethical standards

Ethical approval

Authors obtained prior approval from Research Ethics Committee with the protocol number 03796112.1.0000.0077 as the study design of this research work included human studies.

Informed consent

Informed consent was obtained from all individual participants included in the study.

Conflict of interest

The authors declare that they have no conflict of interest.


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Copyright information

© Springer-Verlag London Ltd., part of Springer Nature 2018

Authors and Affiliations

  • Pam Wen Lung
    • 1
  • Vamshi Krishna Tippavajhala
    • 2
  • Thiago de Oliveira Mendes
    • 3
  • Claudio A. Téllez-Soto
    • 3
  • Desirée Cigaran Schuck
    • 4
  • Carla Abdo Brohem
    • 4
  • Marcio Lorencini
    • 4
  • Airton Abrahão Martin
    • 3
    • 5
  1. 1.Institute of Research and Development (IP&D)Universidade do Vale do Paraiba (UNIVAP)São José dos CamposBrazil
  2. 2.Department of Pharmaceutics, Manipal College of Pharmaceutical SciencesManipal Academy of Higher EducationManipalIndia
  3. 3.Biomedical Engineering Innovation Center, Biomedical Vibrational Spectroscopy GroupUniversidade Brasil-UNBrItaqueraBrazil
  4. 4.Grupo O BoticárioSão José dos PinhaisBrazil
  5. 5.Departmento de FisicaUniversidade Federal do Piaui (UFPI)TeresinaBrazil

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