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Long-pulsed 1064-nm Nd: YAG laser ameliorates LL-37-induced rosacea-like skin lesions through promoting collagen remodeling in BALB/c mice

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Abstract

Long-pulsed 1064-nm neodymium: yttrium-aluminum-garnet laser (LPND) effectively treats rosacea, although the underlying mechanism is unclear, to evaluate the histological effects and molecular mechanism of LPND on LL-37-induced rosacea-like skin lesions in mice. Intradermal injection of LL-37 was performed into the dorsal skin of BALB/c mice (n = 30) twice a day for 2 days. Fifteen mice were treated with LPND. After 48 h, the excised skin sample was stained for histology and type I collagen; transforming growth factor (TGF)-β, matrix metalloproteinase-1 (MMP-1), tissue inhibitor of metalloproteinase (TIMP)-1, tumor necrosis factor (TNF)-α, and interleukin (IL)-1α mRNA levels were determined by real-time RT-PCR. Intradermal injection of LL-37 induced rosacea-like clinical features. LPND treatment significantly reduced erythema and increased dermal collagen production. Levels of Type I collagen, TGF-β, and MMP-1 mRNA were significantly higher in LPND-treated mice than in untreated mice. LPND may improve rosacea by ameliorating dermal connective tissue disorganization and elastosis through MMP-mediated dermal collagen remodeling.

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Acknowledgements

This work was supported by the Basic Science Research program through the National Research Foundation of Korea (NRF), which is funded by the Ministry of Education, Science and Technology and the Ministry of Science, ICT and Future Planning (2015R1C1A2A01055073 and 2016M3D1A1021387).

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Authors and Affiliations

  1. Department of Dermatology, Yeouido St. Mary’s Hospital, College of Medicine, The Catholic University of Korea, 10, 63-ro, Yeongdeungpo-gu, Seoul, 150713, South Korea

    Miri Kim, Jongsic Kim, Seo-won Jeong, Hyunmu Jo & Hyun Jeong Park

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  1. Miri Kim
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  2. Jongsic Kim
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  3. Seo-won Jeong
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  4. Hyunmu Jo
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Correspondence to Hyun Jeong Park.

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The authors declare that they have no conflict of interests.

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This work was performed under specific pathogen-free conditions and approved by the guidelines of the Animal Care Committee of the Catholic University of Korea.

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Kim, M., Kim, J., Jeong, Sw. et al. Long-pulsed 1064-nm Nd: YAG laser ameliorates LL-37-induced rosacea-like skin lesions through promoting collagen remodeling in BALB/c mice. Lasers Med Sci 33, 393–397 (2018). https://doi.org/10.1007/s10103-017-2410-8

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  • DOI: https://doi.org/10.1007/s10103-017-2410-8

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