Effects of photobiomodulation on experimental models of peripheral nerve injury

Abstract

Phototherapy has demonstrated positive effects in the treatment of peripheral nerve injury, but there is a need to investigate the dosimetric parameters. Thus, the aim of the present study was to conduct a literature review on the effects of photobiomodulation with the use of low-level laser therapy (LLLT) on the treatment of peripheral nerve injury in experimental models. The databases of PubMed/MEDLINE, SCOPUS, and SPIE Digital Library were searched for articles on the use of LLLT in experimental models of peripheral nerve injury published in English between January 2007 and March 2016. The laser parameter variability was wavelength (632.8 to 980 nm), power (10 to 190 mW), and total energy (0.15 to 90 J) in pulsed or continuous wave and single or multiple points. Eighteen original articles demonstrating the effects of LLLT on the acceleration of functional recovery, morphological aspects as well as the modulation of the expression inflammatory cytokines, and growth factors were selected. LLLT is a viable phototherapeutic modality for the treatment of peripheral nerve injury, demonstrating positive effects on the neuromuscular repair process using either red or infrared light. The majority of studies used a power of up to 50 mW and total energy of up to 15 J administered to multiple points. The determination of these parameters is important to the standardization of a LLLT protocol to enhance the regeneration process following a peripheral nerve injury.

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References

  1. 1.

    Kim JR, Oh SH, Kwon GB, Namgung U, Song KS, Jeon BH, Lee JH (2013) Acceleration of peripheral nerve regeneration through asymmetrically porous nerve guide conduit applied with biological/physical stimulation. Tissue Eng Part A 19:2674–2685

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  2. 2.

    Li R, Liu Z, Pan Y, Chen L, Zhang Z, Lu L (2014) Peripheral nerve injuries treatment: a systematic review. Cell Biochem Biophys 68:449–454

    CAS  Article  PubMed  Google Scholar 

  3. 3.

    Martínez de Albornos P, Delgado PJ, Forriol F, Maffulli N (2011) Non-surgical therapies for peripheral nerve injury. Br Med Bull 100:73–100

    Article  Google Scholar 

  4. 4.

    Raimondo S, Fornaro M, Di Scipio F, Ronchi G, Giacobini-Robecchi MG, Geuna S (2009) Chapter 5: Methods and protocols in peripheral nerve regeneration experimental research: part II—morphological techniques. Int Rev Neurobiol 87:81–103

    Article  PubMed  Google Scholar 

  5. 5.

    Xiao L, Tsutsui T (2013) Human dental mesenchymal stem cells and neural regeneration. Hum Cell 26:91–96

    CAS  Article  PubMed  Google Scholar 

  6. 6.

    Seddon HS (1975) Surgical disorders of the peripheral nerves, 2nd edn. Churchill Livingstone, Edinburgh

    Google Scholar 

  7. 7.

    Bobinski F, Ferreira TA, Córdova MM, Dombroski PA, da Cunha C, Santo CC, Poli A, Pires RG, Martins-Silva C, Sluka KA, Santos AR (2015) Role of brainstem serotonin in analgesia produced by low-intensity exercise on neuropathic pain after sciatic nerve injury in mice. Pain 156:2595–2606

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  8. 8.

    Dubový P, Brázda V, Klusáková I, Hradilová-Svíženská I (2013) Bilateral elevation of interleukin-6 protein and mRNA in both lumbar and cervical dorsal root ganglia following unilateral chronic compression injury of the sciatic nerve. J Neuroinflammation 10:55

    Article  PubMed  PubMed Central  Google Scholar 

  9. 9.

    Hsieh YL, Chou LW, Chang PL, Yang CC, Kao MJ, Hong CZ (2012) Low-level laser therapy alleviates neuropathic pain and promotes function recovery in rats with chronic constriction injury: possible involvements in hypoxia-inducible factor 1α (HIF-1α). J Comp Neurol 520:2903–2916

    CAS  Article  PubMed  Google Scholar 

  10. 10.

    Silva-Couto MA, Gigo-Benato D, Tim CR, Parizotto NA, Salvini TF, Russo TL (2012) Effects of low-level laser therapy after nerve reconstruction in rat denervated soleus muscle adaptation. Rev Bras Fisioter 16:320–327

    Article  PubMed  Google Scholar 

  11. 11.

    Shen CC, Yang YC, Huang TB, Chan SC, Liu BS (2013) Neural regeneration in a novel nerve conduit across a large gap of the transected sciatic nerve in rats with low-level laser phototherapy. J Biomed Mater Res A 101:2763–2777

    Article  PubMed  Google Scholar 

  12. 12.

    Ijkema-Paassen J, Jansen K, Gramsbergen A, Meek MF (2004) Transection of peripheral nerves, bridging strategies and effect evaluation. Biomaterials 25:1583–1592

    CAS  Article  PubMed  Google Scholar 

  13. 13.

    Pachioni CAS, Mazzer N, Barbieri CH, Fazan VP, Padovani CR, Moro CA, da Silva CA (2006) Rats’ ischiatic nerve injury caused by smashing: a vascularization study. Acta Ortop Bras 14:203–207

    Article  Google Scholar 

  14. 14.

    Rochkind S, Geuna S, Shainberg A (2009) Chapter 25: Phototherapy in peripheral nerve injury: effects on muscle preservation and nerve regeneration. Int Rev Neurobiol 87:445–464

    Article  PubMed  Google Scholar 

  15. 15.

    Passarella S, Karu T (2014) Absorption of monochromatic and narrow band radiation in the visible and near IR by both mitochondrial and non-mitochondrial photoacceptors results in photobiomodulation. J Photochem Photobiol B 140:344–358

    CAS  Article  PubMed  Google Scholar 

  16. 16.

    Karu TI (2008) Mitochondrial signaling in mammalian cells activated by red and near-IR radiation. Photochem Photobiol 84:1091–1099

    CAS  Article  PubMed  Google Scholar 

  17. 17.

    Barbosa RI, Marcolino AM, de Jesus Guirro RR, Mazzer N, Barbieri CH, de Cássia Registro Fonseca M (2010) Comparative effects of wavelengths of low-power laser in regeneration of sciatic nerve in rats following crushing lesion. Lasers Med Sci 25:423–430

    Article  PubMed  Google Scholar 

  18. 18.

    Mohammed IFR, Al-Mustawfi N, Kaka LN (2007) Promotion of regenerative processes in injured peripheral nerve induced by low-level laser therapy. Photomed Laser Surg 25:107–111

    Article  PubMed  Google Scholar 

  19. 19.

    Shen CC, Yang YC, Liu BS (2011) Large-area irradiated low-level laser effect in a biodegradable nerve guide conduit on neural regeneration of peripheral nerve injury in rats. Injury 42:803–813

    Article  PubMed  Google Scholar 

  20. 20.

    Akgul T, Gulsoy M, Gulcur HO (2014) Effects of early and delayed laser application on nerve regeneration. Lasers Med Sci 29:351–357

    Article  PubMed  Google Scholar 

  21. 21.

    Câmara CN, Brito MV, Silveira EL, Silva DS, Simões VR, Pontes RW (2011) Histological analysis of low-intensity laser therapy effects in peripheral nerve regeneration in Wistar rats. Acta Cir Bras 26:12–18

    Article  PubMed  Google Scholar 

  22. 22.

    Gomes LEA, Dalmarco EM, André ES (2012) The brain-derived neurotrophic factor, nerve growth factor, neurotrophin-3, and induced nitric oxide synthase expression after low-level laser therapy in an axonotmesis experimental model. Photomed Laser Surg 11:642–647

    Article  Google Scholar 

  23. 23.

    Alcântara CC, Gigo-Benato D, Salvini TF, Oliveira AL, Anders JJ, Russo TL (2013) Effect of low-level laser therapy (LLLT) on acute neural recovery and inflammation-related gene expression after crush injury in rat sciatic nerve. Lasers Surg Med 45:246–252

    Article  PubMed  Google Scholar 

  24. 24.

    Anders JJ, Moges H, Wu X, Erbele ID, Alberico SL, Saidu EK, Smith JT, Pryor BA (2014) In vitro and in vivo optimization of infrared laser treatment for injured peripheral nerves. Lasers Surg Med 46:34–45

    Article  PubMed  Google Scholar 

  25. 25.

    Moges H, Wu X, McCoy J, Vasconcelos OM, Bryant H, Grunberg NE, Andes JJ (2011) Effect of 810 nm light on nerve regeneration after autograft repair of severely injured rat median nerve. Lasers Surg Med 43:901–906

    Article  PubMed  Google Scholar 

  26. 26.

    Pan HC, Yang DY, Chiu YT, Lai SZ, Wang YC, Chang MH, Cheng FC (2006) Enhanced regeneration in injured sciatic nerve by human amniotic mesenchymal stem cell. J Clin Neurosci 13:570–575

    Article  PubMed  Google Scholar 

  27. 27.

    Askari N, Yaghoobi MM, Shamsara M, Esmaeili-Mahani S (2015) Tetracycline-regulated expression of OLIG2 gene in human dental pulp stem cells lead to mouse sciatic nerve regeneration upon transplantation. Neurosci 305:197–208

    CAS  Article  Google Scholar 

  28. 28.

    Wang CZ, Chen YJ, Wang YH, Yeh ML, Huang MH, Ho ML, Liang JI, Chen CH (2014) Low-level laser irradiation improves functional recovery and nerve regeneration in sciatic nerve crush rat injury model. PLoS One 9:e103348

    Article  PubMed  PubMed Central  Google Scholar 

  29. 29.

    Masoumipoor M, Jameie SB, Janzadeh A, Nasirinezhad F, Soleimani M, Kerdary M (2014) Effects of 660- and 980-nm low-level laser therapy on neuropathic pain relief following chronic constriction injury in rat sciatic nerve. Lasers Med Sci 29:1593–1598

    CAS  Article  PubMed  Google Scholar 

  30. 30.

    Chen YJ, Wang YH, Wang CZ, Ho ML, Kuo PL, Huang MH, Chen CH (2014) Effect of low level laser therapy on chronic compression of the dorsal root ganglion. PLoS One 9:e89894

    Article  PubMed  PubMed Central  Google Scholar 

  31. 31.

    Tos P, Ronchi G, Papalia I, Sallen V, Legagneus J, Geuna S, Giacobini-Robecchi MG (2009) Chapter 4: Methods and protocols in peripheral nerve regeneration experimental research: part I—experimental models. Int Rev Neurobiol 87:47–79

    Article  PubMed  Google Scholar 

  32. 32.

    Rochkind S, Geuna S, Shainberg A (2013) Phototherapy and nerve injury: focus on muscle response. Int Rev Neurobiol 109:99–109

    Article  PubMed  Google Scholar 

  33. 33.

    Belchior ACG, dos Reis FA, Nicolau RA, Silva IS, Pereira DM, de Carvalho PT (2009) Influence of laser (660 nm) on functional recovery of the sciatic nerve in rats following crushing lesion. Lasers Med Sci 24:893–899

    Article  PubMed  Google Scholar 

  34. 34.

    Takhtfooladi MA, Jahanbakhsh F, Takhtfooladi HA, Yousefi K, Allahverdi A (2015) Effect of low-level laser therapy (685 nm, 3 J/cm2) on functional recovery of the sciatic nerve in rats following crushing lesion. Lasers Med Sci 30:1047–1052

    Article  PubMed  Google Scholar 

  35. 35.

    Sousa FF, Andraus RA, Barbieri CH, Mazzer N (2009) Influence of laser radiation in nerve regeneration in different treatments sites. Acta Ortop Bras 17:331–335

    Article  Google Scholar 

  36. 36.

    Medalha CC, Di Gangi GC, Barbosa CB, Fernandes M, Aguiar O, Faloppa F, Leite VM, Rennó AC (2012) Low-level laser therapy improves repair following complete resection of the sciatic nerve in rats. Lasers Med Sci 27:629–635

    Article  PubMed  Google Scholar 

  37. 37.

    Geuna S, Raimondo S, Ronchi G, Di Scipio F, Tos P, Fornaro M (2009) Chapter 3: Histology of the peripheral nerve and chances occurring during nerve regeneration. Int Rev Neurobiol 87:27–46

    Article  PubMed  Google Scholar 

  38. 38.

    Dias FJ, Issa JP, Iyomassa MM, Coutinho-Netto J, Calzzani RA, Iyomassa DM, Sousa LG, Almeida SR, Cury DP, Watanabe IS (2013) Application of a low-level laser therapy and the purified protein from natural latex (Hevea brasiliensis) in the controlled crush injury of the sciatic nerve of rats: a morphological, quantitative, and ultrastructural study. Biomed Res Int 2013:597863

    Article  PubMed  PubMed Central  Google Scholar 

  39. 39.

    Dias FJ, Issa JP, Coutinho-Netto J, Fazan VP, Sousa LG, Iyomassa MM, Papa CP, Watanabe IS (2015) Morphometric and high resolution scanning electron microscopy analysis of low-level laser therapy and latex protein (Hevea brasiliensis) administration following a crush injury of the sciatic nerve in rats. J Neurol Sci 349:129–137

    CAS  Article  PubMed  Google Scholar 

  40. 40.

    dos Reis FA, Belchior AC, de Carvalho PT, da Silva BA, Pereira DM, Silva IS, Nicolau RA (2009) Effect of laser therapy (660 nm) on recovery of the sciatic nerve in rats after injury through neurotmesis followed by epineural anastomosis. Lasers Med Sci 24:741–747

    Article  PubMed  Google Scholar 

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Correspondence to R. A. Mesquita-Ferrari.

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This study received approval from the Ethics Committee on Animal Experimentation of Universidade Nove de Julho under process 2/2016.

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The authors declare that they have no conflict of interest.

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Andreo, L., Soldera, C.B., Ribeiro, B.G. et al. Effects of photobiomodulation on experimental models of peripheral nerve injury. Lasers Med Sci 32, 2155–2165 (2017). https://doi.org/10.1007/s10103-017-2359-7

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Keywords

  • Nerve regeneration
  • Low-level laser therapy
  • Sciatic nerve
  • Photobiomodulation