Skip to main content
SpringerLink
Log in

Rapid analysis of pharmaceutical drugs using LIBS coupled with multivariate analysis

Lasers in Medical Science Aims and scope Submit manuscript

Abstract

Type 2 diabetes drug tablets containing voglibose having dose strengths of 0.2 and 0.3 mg of various brands have been examined, using laser-induced breakdown spectroscopy (LIBS) technique. The statistical methods such as the principal component analysis (PCA) and the partial least square regression analysis (PLSR) have been employed on LIBS spectral data for classifying and developing the calibration models of drug samples. We have developed the ratio-based calibration model applying PLSR in which relative spectral intensity ratios H/C, H/N and O/N are used. Further, the developed model has been employed to predict the relative concentration of element in unknown drug samples. The experiment has been performed in air and argon atmosphere, respectively, and the obtained results have been compared. The present model provides rapid spectroscopic method for drug analysis with high statistical significance for online control and measurement process in a wide variety of pharmaceutical industrial applications.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price includes VAT (France)

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

References

  1. Newmana AW, Byrnb SR (2003) Solid-state analysis of the active pharmaceutical ingredient in drug. Drug Discovery Today 8(19):898–905. https://doi.org/10.1016/S1359-6446(03)02832-0

    Article  Google Scholar 

  2. St-Onge L, Kwong E, Sabsabi M, Vadas EB (2002) Quantitative analysis of pharmaceutical products by laser-induced breakdown spectroscopy. Spectrochimica Acta Part B 57:1131–1140. https://doi.org/10.1016/S0584-8547(02)00062-9

    Article  Google Scholar 

  3. Nancy L (2011) The use of atomic spectroscopy in the pharmaceutical industry for the determination of trace elements in pharmaceuticals. J Pharm Biomed Anal 55:653–661. https://doi.org/10.1016/j.jpba.2010.11.030

    Article  Google Scholar 

  4. Alcalà M et al (2013) Qualitative and quantitative pharmaceutical analysis with a novel hand-held miniature near infrared spectrometer. J Near Infrared Spectroscopy 21:445–457. https://doi.org/10.1255/jnirs.1084

    Article  Google Scholar 

  5. Miziolek AW, Palleschi V, Schechter I (2006) Laser induced breakdown spectroscopy: fundamental and application. Cambridge University Press, New York

    Book  Google Scholar 

  6. Rohit K, Alamelu D, Achrya R, Rai AK (2014) Determination of concentrations of chromium and other elements in soil and plant samples from leather tanning area, instrumental neutron activation analysis. J Radio Anal Nucl Chem 300:213–218. https://doi.org/10.1007/s10967-014-3006-4

    Article  Google Scholar 

  7. Cremers DA, Radziemski LJ (2006) Handbook of laser-induced breakdown spectroscopy. John Willey & sons Ltd, England ISBN: 978-0-470-09300-9

    Book  Google Scholar 

  8. Singh JP, Thakur SN (2007) Laser-induced breakdown spectroscopy. Elsevier, Amslerdam, UK ISBN: 978-0-444-51734-0

    Google Scholar 

  9. Rai AK, Rai VN, Yueh FY, Singh JP (2002) Laser-induced breakdown spectroscopy: a versatile technique for elemental analysis. Trends Appl Spectroscopy 4:165–214

    CAS  Google Scholar 

  10. Dabhi Ajay S, Bhatt Nikita R, Shah Mohit J (2013) Voglibose: an alpha glucosidase inhibitor. J Clin Diagn Res. https://doi.org/10.7860/JCDR/2013/6373.3838

  11. Myakalwara AK et al (2011) Laser-induced breakdown spectroscopy-based investigation and classification of pharmaceutical tablets using multivariate chemometric analysis. Talanta 87:53–59. https://doi.org/10.1016/j.talanta.2011.09.040

    Article  Google Scholar 

  12. Shikha A, Rohit K, Rai GK, Rai AK (2016) Study of archaeological coins of different dynasties using LIBS coupled with multivariate analysis. Opt Lasers Eng 79:29–38. https://doi.org/10.1016/j.optlaseng.2015.11.005

    Article  Google Scholar 

  13. Maurya GS et al (2016) Analysis of impurities on contaminated surface of the tokamak limiter using laser induced breakdown spectroscopy. Spectrochimica Acta PartB: Atomic Spectroscopy 126:17–22. https://doi.org/10.1016/j.sab.2016.10.010

    Article  CAS  Google Scholar 

  14. Archambault J-F et al (2005) The effects of physical parameters on laser-induced breakdown spectroscopy analysis of intact tablets. AAPS Pharm SciTech 6(2):E253–E261. https://doi.org/10.1208/pt060236

    Article  Google Scholar 

  15. Rai AK, Yueh FY, Sing JP (2003) Laser-induced breakdown spectroscopy of molten aluminium alloy. Applied Optics 42(12):2078–2084. https://doi.org/10.1364/AO.42.002078

    Article  CAS  PubMed  Google Scholar 

  16. Kuzuya M, Matsumoto H, Takechi H, Mikami O (1993) Effect of laser energy and atmosphere on the emission characteristics of laser-induced plasma. Appl Spectrosc 47:1659–1664

    Article  CAS  Google Scholar 

  17. Rai S, Rai AK, Thakur SN (2008) Identification of nitro-compounds with LIBS. Applied Physics B: Laser and Optics 91:645. https://doi.org/10.1007/s00340-008-3040-4

    Article  CAS  Google Scholar 

  18. NIST. National Institute of Standards and Technology USA, electronic database, http://physics.nist.gov/physRefData/ASD/lines_form.html

  19. Tognoni E, Palleschi V, Corsi M, Cristoforetti G (2002) Quantitative micro-analysis by laser-induced breakdown spectroscopy: a review of the experimental approaches. Spectrochim Acta B At Spectrosc 57(7):1115–1130. https://doi.org/10.1016/S0584-8547(02)00053-8

    Article  Google Scholar 

  20. Pavel Y, Doug B, Morrison Richard JS, Chadwick BL (2006) A semi-quantitative standard-less analysis method for laser-induced breakdown spectroscopy. Spectrochimica Acta Part B Atomic Spectroscopy 61:200–209. https://doi.org/10.1016/j.sab.2006.01.004

    Article  Google Scholar 

  21. Tran M, Sun Q, Smith BW, Winefordner JD (2001) Determination of F, Cl, and Br in solid organic compounds by laser-induced plasma spectroscopy. Appl Spectrosc 55:739–744

    Article  CAS  Google Scholar 

  22. López-Moreno C et al (2005) Quantitative analysis of low-alloy steel by microchip laser induced breakdown spectroscopy. J Anal At Spectrom 20:552–556. https://doi.org/10.1039/B419173K

    Article  Google Scholar 

  23. Anzano J, Bonilla B et al (2009) Rapid characterization of analgesic pills by LIBS. Med Chem Res. https://doi.org/10.1007/s00044-008-9157-5

  24. Arnab S et al (2015) Evaluation of the prediction precision capability of partial least squares regression approach for analysis of high alloy steel by laser induced breakdown spectroscopy. Spectrochim Acta B 108:8–14. https://doi.org/10.1016/j.sab.2015.04.002

    Article  Google Scholar 

  25. Chan GCY. George, Mao Chan X., Choi I., Sarkar Arnab, Lam O. P., Shuh D. K., Russo Richard E., (2013) Multiple emission line analysis for improved isotopic determination of uranium: a computer simulation study. Spectrochim Acta Part B 89 doi:https://doi.org/10.1016/j.sab.2013.09.001

  26. Jie F, Zhe W, Logan W, Li Z, Ni W (2011) A PLS model based on dominant factor for coal analysis using laser-induced breakdown spectroscopy. Anal Bioanal Chem 400:3261–3271. https://doi.org/10.1007/s00216-011-4865-y

    Article  Google Scholar 

  27. Jovanovic N, Gerich A, Bouchard A, Jiskoot W (2006) Near-infrared imaging for studying homogeneity of protein-sugar mixtures. Pharm Res 23:2002. https://doi.org/10.1007/s11095-006-9037-y

    Article  CAS  PubMed  Google Scholar 

  28. Kadam BR, Jaiswal YS, Shirkhedkar AA, Bari SB (2007) Impurity profile: significance in active pharmaceutical ingredient Eurasian. Eurasian J Anal Chem 2:1306–3057

    Google Scholar 

Download references

Acknowledgements

The authors are thankful to Prof. A N Dwivedi, Department of English, University of Allahabad, to Dr. A K Pathak, Department of Physics, Ewing Christian College, University of Allahabad and to Dr. Abhimanyu Kumar Singh and Dr. G S Maurya, Department of Physics, the University of Allahabad for their valuable suggestions and support. One of the authors, Pravin Kumar Tiwari, is thankful to the University Grant Commission, New Delhi, India, for providing the UGC DPhil fellowship.

Funding

We are not having any financial assistance for this study from any funding bodies.

Author information

Authors and Affiliations

  1. Laser Spectroscopy Research Laboratory, Department of Physics, University of Allahabad, Allahabad, 211002, India

    P. K. Tiwari, S. Awasthi, R. Kumar, R. K. Anand & A. K. Rai

  2. Department of Nephrology, N. 10/60-2, Opal Hospital, DLW Road, Kakarmatta, Varanasi, 221004, India

    P. K. Rai

Authors
  1. P. K. Tiwari
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. S. Awasthi
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. R. Kumar
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. R. K. Anand
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. P. K. Rai
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. A. K. Rai
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to A. K. Rai.

Ethics declarations

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

I would like to inform that the Departmental Programme Committee (DPC), Department of Physics, Faculty of Science, University of Allahabad, and the Research Degree Committee (RDC), University of Allahabad, have approved the topic Study of Pharmaceutical Sample using Spectroscopic techniques for DPhil research work. This is also approved by the Registrar, University of Allahabad. The manuscript Rapid analysis of pharmaceutical drugs using LIBS coupled with multivariate analysis is a part of the abovesaid DPhil degree work. This proves the ethical approval for our study is not required.

Informed consent

We, Pravin Kumar Tiwari, Shikha Awasthi, Rohit Kumar, Raj Kumar Anand, Pradeep Kumar Rai and Awadhesh Kumar Rai, authors of the article Rapid analysis of pharmaceutical drugs using LIBS coupled with multivariate analysis declare that no individual rights are infringed during this study. In our study, we did only compositional analysis of medicine in our laboratory; thus, the medicine (voglibose) has not been used for any clinical purpose.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Tiwari, P.K., Awasthi, S., Kumar, R. et al. Rapid analysis of pharmaceutical drugs using LIBS coupled with multivariate analysis. Lasers Med Sci 33, 263–270 (2018). https://doi.org/10.1007/s10103-017-2358-8

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10103-017-2358-8

Keywords

search

Navigation