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Photobiomodulation therapy improves both inflammatory and fibrotic parameters in experimental model of lung fibrosis in mice

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Abstract

Lung fibrosis (LF) is a chronic and progressive lung disease characterized by pulmonary parenchyma progressive lesion, inflammatory infiltration, and interstitial fibrosis. It is developed by excessive collagen deposition and other cellular matrix components, resulting in severe changes in the alveolar architecture. Considering the absence of effective treatment, the aim of this study was to investigate the effect of photobiomodulation therapy (PBMT) on the development of PF. For this purpose, we used C57BL6 mice subjected to induction of LF by bleomycin administration (1.5 U/kg) by orotracheal route and, after 14 days of the induction, mice were treated with PBMT applied to the thorax 1×/day for 8 days (wavelength 660 ± 20 nm, power 100 mW, radiant exposure 5 J/cm2, irradiance 33.3 mW/cm2, spot size 2.8cm2, total energy 15 J, time of irradiation: 150 s) and inflammatory and fibrotic parameters were evaluated with or without PBMT. Our results showed that PBMT significantly reduced the number of inflammatory cells in the alveolar space, collagen production, interstitial thickening, and static and dynamic pulmonary elastance. In addition, we observed reduced levels of IL-6 e CXCL1/KC released by pneumocytes in culture as well as reduced level of CXCL1/KC released by fibroblasts in culture. We can conclude that the PBMT improves both inflammatory and fibrotic parameters showing a promising therapy which is economical and has no side effects.

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Acknowledgments

We thank Alessandro Melo De Ana by LED device.

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Authors and Affiliations

  1. Post Graduate Program in Biophotonics Applied to Health Sciences, University Nove de Julho (UNINOVE), Rua Vergueiro, 239/245, São Paulo, SP, CEP 01504-000, Brazil

    Robson Alexandre Brochetti, Mayara Peres Leal, Raíssa Rodrigues, Anna Carolina Ratto Tempestini Horliana, Ana Paula Ligeiro de Oliveira & Adriana Lino-dos-Santos-Franco

  2. Post Graduate Program in Science of Rehabilitation, University Nove de Julho (UNINOVE), São Paulo, Brazil

    Renata Kelly da Palma & Luis Vicente Franco de Oliveira

  3. Department of Basic Science in Health, Faculty of Medical Sciences, Federal University of Cuiabá, Cuiabá, Brazil

    Amílcar Sabino Damazo

  4. Laboratory of Pulmonary and Exercise Immunology (LABPEI) and Brazilian Institute of Teaching and Research in Pulmonary and Exercise Immunology (IBEPIPE), São Paulo, Brazil

    Rodolfo Paula Vieira

Authors
  1. Robson Alexandre Brochetti
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  2. Mayara Peres Leal
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  3. Raíssa Rodrigues
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  4. Renata Kelly da Palma
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  5. Luis Vicente Franco de Oliveira
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  6. Anna Carolina Ratto Tempestini Horliana
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  7. Amílcar Sabino Damazo
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  8. Ana Paula Ligeiro de Oliveira
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  9. Rodolfo Paula Vieira
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  10. Adriana Lino-dos-Santos-Franco
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Corresponding author

Correspondence to Adriana Lino-dos-Santos-Franco.

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Competing interests

The authors declare that they have no conflicts of interest.

Funding

This study was sponsored by Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP). Adriana Lino-dos-Santos-Franco is a research fellow from FAPESP (2015/00830-9).

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Brochetti, R.A., Leal, M.P., Rodrigues, R. et al. Photobiomodulation therapy improves both inflammatory and fibrotic parameters in experimental model of lung fibrosis in mice. Lasers Med Sci 32, 1825–1834 (2017). https://doi.org/10.1007/s10103-017-2281-z

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  • DOI: https://doi.org/10.1007/s10103-017-2281-z

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