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Human adipose-derived stem cell spheroid treated with photobiomodulation irradiation accelerates tissue regeneration in mouse model of skin flap ischemia

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Abstract

Skin flap grafting is a form of transplantation widely used in plastic surgery. However, ischemia/reperfusion injury is the main factor which reduces the survival rate of flaps following grafting. We investigated whether photobiomodulation (PBM) precondition prior to human adipose-derived stromal cell (hASC) spheroid (PBM-spheroid) transplantation improved skin tissue functional recovery by the stimulation of angiogenesis and tissue regeneration in skin flap of mice. The LED had an emission wavelength peaked at 660 ± 20 nm (6 J/cm2, 10 mW/cm2). The expression of angiogenic growth factors in PBM-spheroid hASCs was much greater than that of not-PBM-treated spheroid or monolayer-cultured hASCs. From immunochemical staining analysis, the hASCs of PBM-spheroid were CD31+, KDR+, and CD34+, whereas monolayer-cultured hASCs were negative for these markers. To evaluate the therapeutic effect of hASC PBM-spheroid in vivo, PBS, monolayer-cultured hASCs, and not-PBM-spheroid were transplanted into a skin flap model. The animals were observed for 14 days. The PBM-spheroid hASCs transplanted into the skin flap ischemia differentiated into endothelial cells and remained differentiated. Transplantation of PBM-spheroid hASCs into the skin flap ischemia significantly elevated the density of vascular formations through angiogenic factors released by the skin flap ischemia and enhanced tissue regeneration at the lesion site. Consistent with these results, the transplantation of PBM-spheroid hASCs significantly improved functional recovery compared with PBS, monolayer-cultured hASCs, and not-PBM-spheroid treatment. These findings suggest that transplantation of PBM-spheroid hASCs may be an effective stem cell therapy for the treatment of skin flap ischemia.

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Authors and Affiliations

  1. Beckman Laser Institute Korea, Dankook University, 119 Dandae-ro, Cheonan, Chungnam, 330-714, South Korea

    In-Su Park, Phil-Sang Chung & Jin Chul Ahn

  2. Department of Otolaryngology-Head and Neck Surgery, College of Medicine, Dankook University, 119 Dandae-ro, Cheonan, Chungnam, 330-714, South Korea

    Phil-Sang Chung

  3. Department of Biomedical Science, Dankook University, Cheonan, Chungnam, 330-714, South Korea

    Jin Chul Ahn

  4. Biomedical Translational Research Institute, Dankook University, Cheonan, Chungnam, 330-714, South Korea

    Jin Chul Ahn

  5. University of California, Irvine, CA, USA

    Anais Leproux

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  1. In-Su Park
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  2. Phil-Sang Chung
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  4. Anais Leproux
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Correspondence to Phil-Sang Chung.

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Research funder: Research Support Foundation of Ministry of Science, ICT, and Future Planning grant, Basic Science Research Program; Grant number: 2012K1A4A3053142, NRF-2014R1A1A1038199, 2015R1D1A1A02062326, HI14C2161.

The authors declare that they have no conflict of interest with the Research Foundation.

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Park, IS., Chung, PS., Ahn, J.C. et al. Human adipose-derived stem cell spheroid treated with photobiomodulation irradiation accelerates tissue regeneration in mouse model of skin flap ischemia. Lasers Med Sci 32, 1737–1746 (2017). https://doi.org/10.1007/s10103-017-2239-1

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  • DOI: https://doi.org/10.1007/s10103-017-2239-1

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